FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146596247> ?p ?o ?g. }

• W2146596247 endingPage "e81" @default.
• W2146596247 startingPage "e81" @default.
• W2146596247 abstract "The use of oligonucleotide-assisted cleavage and ligation (ONCL), a novel approach to the capture of gene repertoires, in the construction of a phage-display immune antibody library is described. ONCL begins with rapid amplification of cDNA ends to amplify all members equally. A single, specific cut near 5′ and/or 3′ end of each gene fragment (in single stranded form) is facilitated by hybridization with an appropriate oligonucleotide adapter. Directional cloning of targeted DNA is accomplished by ligation of a partially duplex DNA molecule (containing suitable restriction sites) and amplification with primers in constant regions. To demonstrate utility and reliability of ONCL, a human antibody repertoire was cloned from IgG mRNA extracted from human B-lymphocytes engrafted in Trimera mice. These mice were transplanted with peripheral blood lymphocytes from Candida albicans infected individuals and subsequently immunized with C.albicans glyceraldehyde-3-phosphate dehydrogenase (GAPDH). DNA sequencing showed that ONCL resulted in efficient capture of gene repertoires. Indeed, full representation of all V H families/segments was observed showing that ONCL did not introduce cloning biases for or against any V H family. We validated the efficiency of ONCL by creating a functional Fab phage-display library with a size of 3.3 × 10 10 and by selecting five unique Fabs against GAPDH antigen." @default.
• W2146596247 created "2016-06-24" @default.
• W2146596247 creator A5083248117 @default.
• W2146596247 date "2005-05-16" @default.
• W2146596247 modified "2023-10-10" @default.
• W2146596247 title "Oligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display library" @default.
• W2146596247 cites W1489538658 @default.
• W2146596247 cites W1531785323 @default.
• W2146596247 cites W1549258100 @default.
• W2146596247 cites W1601908524 @default.
• W2146596247 cites W1606431180 @default.
• W2146596247 cites W1975512139 @default.
• W2146596247 cites W1980352910 @default.
• W2146596247 cites W1990299097 @default.
• W2146596247 cites W1996391238 @default.
• W2146596247 cites W1997830689 @default.
• W2146596247 cites W2006174330 @default.
• W2146596247 cites W2012631683 @default.
• W2146596247 cites W2021673452 @default.
• W2146596247 cites W2025827008 @default.
• W2146596247 cites W2050717506 @default.
• W2146596247 cites W2052332203 @default.
• W2146596247 cites W2053288839 @default.
• W2146596247 cites W2056374924 @default.
• W2146596247 cites W2066683596 @default.
• W2146596247 cites W2067977668 @default.
• W2146596247 cites W2071378581 @default.
• W2146596247 cites W2083284221 @default.
• W2146596247 cites W2085881249 @default.
• W2146596247 cites W2090057847 @default.
• W2146596247 cites W2091572749 @default.
• W2146596247 cites W2093380070 @default.
• W2146596247 cites W2105364445 @default.
• W2146596247 cites W2105480051 @default.
• W2146596247 cites W2107312700 @default.
• W2146596247 cites W2108388593 @default.
• W2146596247 cites W2118369672 @default.
• W2146596247 cites W2120161758 @default.
• W2146596247 cites W2121732949 @default.
• W2146596247 cites W2134202212 @default.
• W2146596247 cites W2148312059 @default.
• W2146596247 cites W2175687390 @default.
• W2146596247 cites W2407636941 @default.
• W2146596247 cites W2749679400 @default.
• W2146596247 doi "https://doi.org/10.1093/nar/gni080" @default.
• W2146596247 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1131936" @default.
• W2146596247 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15905471" @default.
• W2146596247 hasPublicationYear "2005" @default.
• W2146596247 type Work @default.
• W2146596247 sameAs 2146596247 @default.
• W2146596247 citedByCount "8" @default.
• W2146596247 countsByYear W21465962472012 @default.
• W2146596247 crossrefType "journal-article" @default.
• W2146596247 hasAuthorship W2146596247A5083248117 @default.
• W2146596247 hasBestOaLocation W21465962471 @default.
• W2146596247 hasConcept C10079403 @default.
• W2146596247 hasConcept C104317684 @default.
• W2146596247 hasConcept C119599485 @default.
• W2146596247 hasConcept C121050878 @default.
• W2146596247 hasConcept C127413603 @default.
• W2146596247 hasConcept C128319531 @default.
• W2146596247 hasConcept C129312508 @default.
• W2146596247 hasConcept C13575015 @default.
• W2146596247 hasConcept C153911025 @default.
• W2146596247 hasConcept C159654299 @default.
• W2146596247 hasConcept C167625842 @default.
• W2146596247 hasConcept C177284502 @default.
• W2146596247 hasConcept C186268636 @default.
• W2146596247 hasConcept C187882448 @default.
• W2146596247 hasConcept C19924922 @default.
• W2146596247 hasConcept C199360897 @default.
• W2146596247 hasConcept C2776441376 @default.
• W2146596247 hasConcept C41008148 @default.
• W2146596247 hasConcept C54355233 @default.
• W2146596247 hasConcept C547475151 @default.
• W2146596247 hasConcept C86803240 @default.
• W2146596247 hasConceptScore W2146596247C10079403 @default.
• W2146596247 hasConceptScore W2146596247C104317684 @default.
• W2146596247 hasConceptScore W2146596247C119599485 @default.
• W2146596247 hasConceptScore W2146596247C121050878 @default.
• W2146596247 hasConceptScore W2146596247C127413603 @default.
• W2146596247 hasConceptScore W2146596247C128319531 @default.
• W2146596247 hasConceptScore W2146596247C129312508 @default.
• W2146596247 hasConceptScore W2146596247C13575015 @default.
• W2146596247 hasConceptScore W2146596247C153911025 @default.
• W2146596247 hasConceptScore W2146596247C159654299 @default.
• W2146596247 hasConceptScore W2146596247C167625842 @default.
• W2146596247 hasConceptScore W2146596247C177284502 @default.
• W2146596247 hasConceptScore W2146596247C186268636 @default.
• W2146596247 hasConceptScore W2146596247C187882448 @default.
• W2146596247 hasConceptScore W2146596247C19924922 @default.
• W2146596247 hasConceptScore W2146596247C199360897 @default.
• W2146596247 hasConceptScore W2146596247C2776441376 @default.
• W2146596247 hasConceptScore W2146596247C41008148 @default.
• W2146596247 hasConceptScore W2146596247C54355233 @default.
• W2146596247 hasConceptScore W2146596247C547475151 @default.
• W2146596247 hasConceptScore W2146596247C86803240 @default.
• W2146596247 hasIssue "9" @default.

• next