FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146608717> ?p ?o ?g. }

• W2146608717 endingPage "6885" @default.
• W2146608717 startingPage "6878" @default.
• W2146608717 abstract "Pseudomonas syringae pv. syringae 61 contains a 25-kb cluster of hrp genes that are required for elicitation of the hypersensitive response (HR) in tobacco. TnphoA mutagenesis of cosmid pHIR11, which contains the hrp cluster, revealed two genes encoding exported or inner-membrane-spanning proteins (H.-C. Huang, S. W. Hutcheson, and A. Collmer, Mol. Plant-Microbe Interact. 4:469-476, 1991). The gene in complementation group X, designated hrpH, was subcloned on a 3.1-kb SalI fragment into pCPP30, a broad-host-range, mobilizable vector. The subclone restored the ability of hrpH mutant P. syringae pv. syringae 61-2089 to elicit the HR in tobacco. DNA sequence analysis of the 3.1-kb SalI fragment revealed a single open reading frame encoding an 81,956-Da preprotein with a typical amino-terminal signal peptide and no likely inner-membrane-spanning hydrophobic regions. hrpH was expressed in the presence of [35S]methionine by using the T7 RNA polymerase-promoter system and vector pT7-3 in Escherichia coli and was shown to encode a protein with an apparent molecular weight of 83,000 on sodium dodecyl sulfate-polyacrylamide gels. The HrpH protein in E. coli was located in the membrane fraction and was absent from the periplasm and cytoplasm. The HrpH protein possessed similarity with several outer membrane proteins that are known to be involved in protein or phage secretion, including the Klebsiella oxytoca PulD protein, the Yersinia enterocolitica YscC protein, and the pIV protein of filamentous coliphages. All of these proteins possess a possible secretion motif, GG(X)12VP(L/F)LXXIPXIGXL(F/L), near the carboxyl terminus, and they lack a carboxyl-terminal phenylalanine, in contrast to other outer membrane proteins with no known secretion function. These results suggest that the P. syringae pv. syringae HrpH protein is involved in the secretion of a proteinaceous HR elicitor." @default.
• W2146608717 created "2016-06-24" @default.
• W2146608717 creator A5040115265 @default.
• W2146608717 creator A5069579045 @default.
• W2146608717 creator A5072753808 @default.
• W2146608717 creator A5086773186 @default.
• W2146608717 date "1992-11-01" @default.
• W2146608717 modified "2023-09-26" @default.
• W2146608717 title "The Pseudomonas syringae pv. syringae 61 hrpH product, an envelope protein required for elicitation of the hypersensitive response in plants" @default.
• W2146608717 cites W1557633902 @default.
• W2146608717 cites W1603365990 @default.
• W2146608717 cites W1730597761 @default.
• W2146608717 cites W1763493835 @default.
• W2146608717 cites W1838285010 @default.
• W2146608717 cites W1925709767 @default.
• W2146608717 cites W1939201264 @default.
• W2146608717 cites W1945068486 @default.
• W2146608717 cites W1964633546 @default.
• W2146608717 cites W1987631910 @default.
• W2146608717 cites W2005654501 @default.
• W2146608717 cites W2009310436 @default.
• W2146608717 cites W2013096292 @default.
• W2146608717 cites W2015292449 @default.
• W2146608717 cites W2017316239 @default.
• W2146608717 cites W2028622989 @default.
• W2146608717 cites W2030353309 @default.
• W2146608717 cites W2036298006 @default.
• W2146608717 cites W2042202066 @default.
• W2146608717 cites W2048101669 @default.
• W2146608717 cites W2055600356 @default.
• W2146608717 cites W2068518785 @default.
• W2146608717 cites W2113790807 @default.
• W2146608717 cites W2124514053 @default.
• W2146608717 cites W2131282088 @default.
• W2146608717 cites W2138879032 @default.
• W2146608717 cites W2140514518 @default.
• W2146608717 cites W2147169435 @default.
• W2146608717 cites W2157924633 @default.
• W2146608717 cites W2158797858 @default.
• W2146608717 cites W2168144296 @default.
• W2146608717 cites W2331153448 @default.
• W2146608717 cites W2462469424 @default.
• W2146608717 cites W3021558072 @default.
• W2146608717 doi "https://doi.org/10.1128/jb.174.21.6878-6885.1992" @default.
• W2146608717 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/207366" @default.
• W2146608717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1400238" @default.
• W2146608717 hasPublicationYear "1992" @default.
• W2146608717 type Work @default.
• W2146608717 sameAs 2146608717 @default.
• W2146608717 citedByCount "109" @default.
• W2146608717 countsByYear W21466087172012 @default.
• W2146608717 countsByYear W21466087172013 @default.
• W2146608717 countsByYear W21466087172014 @default.
• W2146608717 countsByYear W21466087172015 @default.
• W2146608717 countsByYear W21466087172016 @default.
• W2146608717 countsByYear W21466087172018 @default.
• W2146608717 countsByYear W21466087172019 @default.
• W2146608717 countsByYear W21466087172020 @default.
• W2146608717 countsByYear W21466087172021 @default.
• W2146608717 countsByYear W21466087172022 @default.
• W2146608717 countsByYear W21466087172023 @default.
• W2146608717 crossrefType "journal-article" @default.
• W2146608717 hasAuthorship W2146608717A5040115265 @default.
• W2146608717 hasAuthorship W2146608717A5069579045 @default.
• W2146608717 hasAuthorship W2146608717A5072753808 @default.
• W2146608717 hasAuthorship W2146608717A5086773186 @default.
• W2146608717 hasBestOaLocation W21466087171 @default.
• W2146608717 hasConcept C104317684 @default.
• W2146608717 hasConcept C10858879 @default.
• W2146608717 hasConcept C143065580 @default.
• W2146608717 hasConcept C146587185 @default.
• W2146608717 hasConcept C153911025 @default.
• W2146608717 hasConcept C167625842 @default.
• W2146608717 hasConcept C188082640 @default.
• W2146608717 hasConcept C201663137 @default.
• W2146608717 hasConcept C2780381237 @default.
• W2146608717 hasConcept C547475151 @default.
• W2146608717 hasConcept C55493867 @default.
• W2146608717 hasConcept C86803240 @default.
• W2146608717 hasConceptScore W2146608717C104317684 @default.
• W2146608717 hasConceptScore W2146608717C10858879 @default.
• W2146608717 hasConceptScore W2146608717C143065580 @default.
• W2146608717 hasConceptScore W2146608717C146587185 @default.
• W2146608717 hasConceptScore W2146608717C153911025 @default.
• W2146608717 hasConceptScore W2146608717C167625842 @default.
• W2146608717 hasConceptScore W2146608717C188082640 @default.
• W2146608717 hasConceptScore W2146608717C201663137 @default.
• W2146608717 hasConceptScore W2146608717C2780381237 @default.
• W2146608717 hasConceptScore W2146608717C547475151 @default.
• W2146608717 hasConceptScore W2146608717C55493867 @default.
• W2146608717 hasConceptScore W2146608717C86803240 @default.
• W2146608717 hasIssue "21" @default.
• W2146608717 hasLocation W21466087171 @default.
• W2146608717 hasLocation W21466087172 @default.
• W2146608717 hasLocation W21466087173 @default.
• W2146608717 hasLocation W21466087174 @default.
• W2146608717 hasOpenAccess W2146608717 @default.
• W2146608717 hasPrimaryLocation W21466087171 @default.

• next