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• W2146635663 abstract "Abstract In the in vitro system, in which nuclei from liver of control rats are incubated with surrogate cytoplasm containing cytosol from liver of rats which received by stomach tube 4 mg of 3′-methyl-4-dimethylaminoazobenzene (3′-MeDAB) 40 hr earlier, some of the azocarcinogen is transferred from the cytosol into the nuclei. This transfer does not require the addition of an energy source into the incubation mixture but is temperature dependent and requires the presence of Mg 2+ . The transported 3′-MeDAB is bound to macromolecules present in the cytosol. The azocarcinogen is transferred most efficiently by a fraction of cytosol proteins which are retained on carboxymethylcellulose. In the same in vitro system, the release of labeled RNA from nuclei of liver of animals which were prelabeled in vivo for 30 min with [ 3 H]orotic acid is temperature dependent and requires the addition of adenosine 5′-triphosphate into the surrogate cytoplasm. The labeled RNA is released in the form of 30 to 35S ribonucleoprotein particles with a mean buoyant density of 1.47 g/cu cm. No release of labeled ribosomal or preribosomal ribonucleoprotein particles was detected. The incubation of nuclei from normal rat liver with surrogate cytoplasm containing cytosol from liver of animals treated with 3′-MeDAB 40 hr earlier does not change the temperature or energy dependence of the release of RNA from the nuclei. Neither is there a change in the size of the released ribonucleoprotein particles nor in the rate of the RNA released into the surrogate cytoplasm during the first 20 min of incubation of isolated nuclei in vitro . But in comparison to the incubation of nuclei from normal rat liver with homologous cytosol where the release of RNA from the nuclei essentially ceased after 30 min of incubation, in the presence of cytosol from 3′-MeDAB-treated rats, the release continued during the whole tested 60-min incubation period without losing its requirements for the addition of an external energy source. The immediate effect of the azocarcinogen present in the cytosol on the processing and transport of nuclear RNA was demonstrated by its induction of the release from the nuclei of normal rat liver into the surrogate cytoplasm of nucleotide sequences of small-molecular-weight chromosomal Fraction 3 RNA. Sequences of this RNA are in the liver of control animals restricted to the nucleus and are also not released in vitro if liver nuclei from normal rats are incubated with homologous cytosol. The release of sequences of chromosomal Fraction 3 RNA from nuclei in vitro in the presence of cytosol from animals treated with 3′-MeDAB resembles the effect observed in vivo in liver of animals fed 3′-MeDAB. The presence of the sequences of the metabolically active chromosomal Fraction 3 RNA in the released ribonucleoprotein particles agrees with the conclusion reached from in vivo experiments (Patel, N. T., Folse, D. S., and Holoubek, V. Cancer Res., 39: 4460–4465, 1979) that the azocarcinogen also interferes with the processing of nuclear RNA. The results of the experiments in vitro infer that the cytosol contains nondialyzable component that regulates the nuclear processing and transport of messenger RNA. This component is either inactivated by the azocarcinogen or transfers this carcinogen into the cell nucleus with the resulting distortion of RNA processing in the cell nucleus." @default.
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• W2146635663 date "1981-03-01" @default.
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• W2146635663 title "In vitro incorporation of 3'-methyl-4-dimethylaminoazobenzene into liver nuclei and release of RNA from the nuclei." @default.
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