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- W2146646899 abstract "The insoluble protein-dye complex, Azocoll, can be used to measure proteolytic activity as a function of time. By using the aliquoted method discussed in this report, one 5.0 ml reaction mixture can give up to five points on a standard velocity curve. It has been shown that the ratio of solid to liquid in the reaction mixture can be kept constant to give accurate values when pipetting aliquots from a solid-liquid mixture. We have also shown that Azocoll is more sensitive to trypsin than previously reported and that the “dye bleeding” in control samples can be kept at a manageable level." @default.
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- W2146646899 date "1969-10-01" @default.
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- W2146646899 title "Use of azo-dye-bound collagen to measure reaction velocities of proteolytic enzymes" @default.
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- W2146646899 doi "https://doi.org/10.1016/0003-2697(69)90111-0" @default.
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