FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146704705> ?p ?o ?g. }

• W2146704705 endingPage "4339" @default.
• W2146704705 startingPage "4331" @default.
• W2146704705 abstract "Most species of the diazotrophic Proteobacteria Azoarcus spp. occur in association with grass roots, while A. tolulyticus and A. evansii are soil bacteria not associated with a plant host. To facilitate species identification and strain comparison, we developed a protocol for PCR-generated genomic fingerprints, using an automated sequencer for fragment analysis. Commonly used primers targeted to REP (repetitive extragenic palindromic) and ERIC (enterobacterial repetitive intergenic consensus) sequence elements failed to amplify fragments from the two species tested. In contrast, the BOX-PCR assay (targeted to repetitive intergenic sequence elements of Streptococcus) yielded species-specific genomic fingerprints with some strain-specific differences. PCR profiles of an additional PCR assay using primers targeted to tRNA genes (tDNA-PCR, for tRNA(IIe)) were more discriminative, allowing differentiation at species-specific (for two species) or infraspecies-specific level. Our protocol of several consecutive PCR assays consisted of 16S ribosomal DNA (rDNA)-targeted, genus-specific PCR followed by BOX- and tDNA-PCR; it enabled us to assign new diazotrophic isolates originating from fungal resting stages (sclerotia) to known species of Azoarcus. The assignment was confirmed by phylogenetic analysis of 16S rDNA sequences. Additionally, the phylogenetic distances and the lack of monophyly suggested emendment of the genus Azoarcus: the unnamed species Azoarcus groups C and D and a new group (E) of Azoarcus, which was detected in association with fungi, are likely to have the taxonomic rank of three different genera. According to its small subunit rRNA, the sclerotium-forming basidiomycete was related to the Ustilagomycetes, facultatively biotrophic parasites of plants. Since they occurred in a field which was under cultivation with rice and wheat, these fungi might serve as a niche for survival for Azoarcus in the soil and as a source for reinfection of plants." @default.
• W2146704705 created "2016-06-24" @default.
• W2146704705 creator A5003061848 @default.
• W2146704705 creator A5010344664 @default.
• W2146704705 creator A5067237611 @default.
• W2146704705 date "1997-11-01" @default.
• W2146704705 modified "2023-09-23" @default.
• W2146704705 title "Identification of N2-fixing plant- and fungus-associated Azoarcus species by PCR-based genomic fingerprints" @default.
• W2146704705 cites W1481831465 @default.
• W2146704705 cites W1502658707 @default.
• W2146704705 cites W1511757854 @default.
• W2146704705 cites W1521703296 @default.
• W2146704705 cites W1525734744 @default.
• W2146704705 cites W1543289866 @default.
• W2146704705 cites W1548706496 @default.
• W2146704705 cites W1557830917 @default.
• W2146704705 cites W1566089326 @default.
• W2146704705 cites W1581642869 @default.
• W2146704705 cites W159335903 @default.
• W2146704705 cites W1594529235 @default.
• W2146704705 cites W1755654232 @default.
• W2146704705 cites W1772054043 @default.
• W2146704705 cites W1800445109 @default.
• W2146704705 cites W1853243092 @default.
• W2146704705 cites W1854571056 @default.
• W2146704705 cites W1869310903 @default.
• W2146704705 cites W1886645152 @default.
• W2146704705 cites W1953897354 @default.
• W2146704705 cites W1996107392 @default.
• W2146704705 cites W2002463127 @default.
• W2146704705 cites W2008669354 @default.
• W2146704705 cites W2016000389 @default.
• W2146704705 cites W2027246443 @default.
• W2146704705 cites W2030062456 @default.
• W2146704705 cites W2045770689 @default.
• W2146704705 cites W2049640286 @default.
• W2146704705 cites W2051839868 @default.
• W2146704705 cites W2056485579 @default.
• W2146704705 cites W2082317013 @default.
• W2146704705 cites W2082525069 @default.
• W2146704705 cites W2100228506 @default.
• W2146704705 cites W2102274379 @default.
• W2146704705 cites W2119782349 @default.
• W2146704705 cites W2120644786 @default.
• W2146704705 cites W2124704515 @default.
• W2146704705 cites W2129334025 @default.
• W2146704705 cites W2132598047 @default.
• W2146704705 cites W2139434859 @default.
• W2146704705 cites W2146018009 @default.
• W2146704705 cites W2146456021 @default.
• W2146704705 cites W2149767381 @default.
• W2146704705 cites W2152361337 @default.
• W2146704705 cites W2157239141 @default.
• W2146704705 cites W2167472164 @default.
• W2146704705 cites W2167673850 @default.
• W2146704705 cites W2170824069 @default.
• W2146704705 cites W2317907906 @default.
• W2146704705 doi "https://doi.org/10.1128/aem.63.11.4331-4339.1997" @default.
• W2146704705 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/168753" @default.
• W2146704705 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9361420" @default.
• W2146704705 hasPublicationYear "1997" @default.
• W2146704705 type Work @default.
• W2146704705 sameAs 2146704705 @default.
• W2146704705 citedByCount "127" @default.
• W2146704705 countsByYear W21467047052012 @default.
• W2146704705 countsByYear W21467047052013 @default.
• W2146704705 countsByYear W21467047052014 @default.
• W2146704705 countsByYear W21467047052015 @default.
• W2146704705 countsByYear W21467047052016 @default.
• W2146704705 countsByYear W21467047052017 @default.
• W2146704705 countsByYear W21467047052018 @default.
• W2146704705 countsByYear W21467047052019 @default.
• W2146704705 countsByYear W21467047052020 @default.
• W2146704705 countsByYear W21467047052023 @default.
• W2146704705 crossrefType "journal-article" @default.
• W2146704705 hasAuthorship W2146704705A5003061848 @default.
• W2146704705 hasAuthorship W2146704705A5010344664 @default.
• W2146704705 hasAuthorship W2146704705A5067237611 @default.
• W2146704705 hasBestOaLocation W21467047051 @default.
• W2146704705 hasConcept C104317684 @default.
• W2146704705 hasConcept C141231307 @default.
• W2146704705 hasConcept C17757408 @default.
• W2146704705 hasConcept C193252679 @default.
• W2146704705 hasConcept C195139083 @default.
• W2146704705 hasConcept C207812311 @default.
• W2146704705 hasConcept C42062724 @default.
• W2146704705 hasConcept C54355233 @default.
• W2146704705 hasConcept C67905577 @default.
• W2146704705 hasConcept C86803240 @default.
• W2146704705 hasConcept C90132467 @default.
• W2146704705 hasConceptScore W2146704705C104317684 @default.
• W2146704705 hasConceptScore W2146704705C141231307 @default.
• W2146704705 hasConceptScore W2146704705C17757408 @default.
• W2146704705 hasConceptScore W2146704705C193252679 @default.
• W2146704705 hasConceptScore W2146704705C195139083 @default.
• W2146704705 hasConceptScore W2146704705C207812311 @default.
• W2146704705 hasConceptScore W2146704705C42062724 @default.
• W2146704705 hasConceptScore W2146704705C54355233 @default.

• next