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• W2146749523 abstract "Abstract Six different clones of 1‐year‐old loblolly pine ( Pinus taeda L.) seedlings grown under standardized conditions in a green house were used for sample preparation and further analysis. Three independent and complementary analytical techniques for metabolic profiling were applied in the present study: hydrophilic interaction chromatography (HILIC‐LC/ESI‐MS), reversed‐phase liquid chromatography (RP‐LC/ESI‐MS), and gas chromatography all coupled to mass spectrometry (GC/TOF‐MS). Unsupervised methods, such as principle component analysis (PCA) and clustering, and supervised methods, such as classification, were used for data mining. Genetic algorithms (GA), a multivariate approach, was probed for selection of the smallest subsets of potentially discriminative classifiers. From more than 2000 peaks found in total, small subsets were selected by GA as highly potential classifiers allowing discrimination among six investigated genotypes. Annotated GC/TOF‐MS data allowed the generation of a small subset of identified metabolites. LC/ESI‐MS data and small subsets require further annotation. The present study demonstrated that combination of comprehensive metabolic profiling and advanced data mining techniques provides a powerful metabolomic approach for biomarker discovery among small molecules. Utilizing GA for feature selection allowed the generation of small subsets of potent classifiers. Copyright © 2008 John Wiley & Sons, Ltd." @default.
• W2146749523 created "2016-06-24" @default.
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• W2146749523 date "2008-03-27" @default.
• W2146749523 modified "2023-10-11" @default.
• W2146749523 title "Probing genetic algorithms for feature selection in comprehensive metabolic profiling approach" @default.
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• W2146749523 doi "https://doi.org/10.1002/rcm.3507" @default.
• W2146749523 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18383216" @default.
• W2146749523 hasPublicationYear "2008" @default.
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