Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146819180> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2146819180 endingPage "45" @default.
- W2146819180 startingPage "37" @default.
- W2146819180 abstract "Germinal vesicle (GV)-stage horse oocytes with diffuse chromatin are meiotically incompetent and degenerate in culture, whereas horse oocytes having condensed chromatin within the GV are meiotically competent. Degeneration of incompetent oocytes in culture may be related to premature GV breakdown, which could possibly be prevented by inhibition of m-phase protein activity. We examined the effects of 6-dimethylaminopurine (6-DMAP), butyrolactone and roscovitine on GV-stage horse oocytes. Culture in the presence of 2 mM 6-DMAP for 24 h suppressed meiosis (2% MI or MII compared with 38% for untreated oocytes). The proportion of GV-stage oocytes having condensed chromatin was not different between 6-DMAP culture and directly fixed controls; however, the proportion of oocytes with diffuse chromatin was significantly lower, and more oocytes with diffuse chromatin had atypical chromatin than did controls (p < 0.01). Culture with butyrolactone at 100 mM suppressed meiosis (5% MI + II). Again, this treatment maintained GV-stage oocytes having condensed chromatin, but the proportion of oocytes with diffuse chromatin was significantly reduced compared with directly fixed controls (p < 0.05). Culture with roscovitine at 25 μM was also effective in maintaining GV-stage oocytes having condensed chromatin; however, culture with 100 μM roscovitine did not suppress meiosis or maintain oocytes in the GV stage. These results indicate that meiosis in GV-stage horse oocytes having condensed chromatin may be suppressed by inhibitors of m-phase protein activity; however, oocytes originally having diffuse chromatin appear to degenerate in culture even in the presence of these inhibitors." @default.
- W2146819180 created "2016-06-24" @default.
- W2146819180 creator A5022310140 @default.
- W2146819180 creator A5049175374 @default.
- W2146819180 creator A5066297499 @default.
- W2146819180 creator A5072273602 @default.
- W2146819180 creator A5073427041 @default.
- W2146819180 creator A5090253858 @default.
- W2146819180 date "2002-02-01" @default.
- W2146819180 modified "2023-09-30" @default.
- W2146819180 title "Suppression of meiosis by inhibitors of m-phase proteins in horse oocytes with low meiotic competence" @default.
- W2146819180 doi "https://doi.org/10.1017/s096719940200206x" @default.
- W2146819180 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11964090" @default.
- W2146819180 hasPublicationYear "2002" @default.
- W2146819180 type Work @default.
- W2146819180 sameAs 2146819180 @default.
- W2146819180 citedByCount "12" @default.
- W2146819180 countsByYear W21468191802015 @default.
- W2146819180 countsByYear W21468191802020 @default.
- W2146819180 countsByYear W21468191802022 @default.
- W2146819180 crossrefType "journal-article" @default.
- W2146819180 hasAuthorship W2146819180A5022310140 @default.
- W2146819180 hasAuthorship W2146819180A5049175374 @default.
- W2146819180 hasAuthorship W2146819180A5066297499 @default.
- W2146819180 hasAuthorship W2146819180A5072273602 @default.
- W2146819180 hasAuthorship W2146819180A5073427041 @default.
- W2146819180 hasAuthorship W2146819180A5090253858 @default.
- W2146819180 hasConcept C104317684 @default.
- W2146819180 hasConcept C120504264 @default.
- W2146819180 hasConcept C148196450 @default.
- W2146819180 hasConcept C153911025 @default.
- W2146819180 hasConcept C16685009 @default.
- W2146819180 hasConcept C185592680 @default.
- W2146819180 hasConcept C196843134 @default.
- W2146819180 hasConcept C2776690073 @default.
- W2146819180 hasConcept C29537977 @default.
- W2146819180 hasConcept C2994119143 @default.
- W2146819180 hasConcept C54355233 @default.
- W2146819180 hasConcept C552990157 @default.
- W2146819180 hasConcept C6902291 @default.
- W2146819180 hasConcept C71924100 @default.
- W2146819180 hasConcept C83640560 @default.
- W2146819180 hasConcept C86803240 @default.
- W2146819180 hasConcept C95444343 @default.
- W2146819180 hasConceptScore W2146819180C104317684 @default.
- W2146819180 hasConceptScore W2146819180C120504264 @default.
- W2146819180 hasConceptScore W2146819180C148196450 @default.
- W2146819180 hasConceptScore W2146819180C153911025 @default.
- W2146819180 hasConceptScore W2146819180C16685009 @default.
- W2146819180 hasConceptScore W2146819180C185592680 @default.
- W2146819180 hasConceptScore W2146819180C196843134 @default.
- W2146819180 hasConceptScore W2146819180C2776690073 @default.
- W2146819180 hasConceptScore W2146819180C29537977 @default.
- W2146819180 hasConceptScore W2146819180C2994119143 @default.
- W2146819180 hasConceptScore W2146819180C54355233 @default.
- W2146819180 hasConceptScore W2146819180C552990157 @default.
- W2146819180 hasConceptScore W2146819180C6902291 @default.
- W2146819180 hasConceptScore W2146819180C71924100 @default.
- W2146819180 hasConceptScore W2146819180C83640560 @default.
- W2146819180 hasConceptScore W2146819180C86803240 @default.
- W2146819180 hasConceptScore W2146819180C95444343 @default.
- W2146819180 hasIssue "1" @default.
- W2146819180 hasLocation W21468191801 @default.
- W2146819180 hasLocation W21468191802 @default.
- W2146819180 hasOpenAccess W2146819180 @default.
- W2146819180 hasPrimaryLocation W21468191801 @default.
- W2146819180 hasRelatedWork W1986278964 @default.
- W2146819180 hasRelatedWork W2010358446 @default.
- W2146819180 hasRelatedWork W2059552522 @default.
- W2146819180 hasRelatedWork W2061309896 @default.
- W2146819180 hasRelatedWork W2070542124 @default.
- W2146819180 hasRelatedWork W2152752168 @default.
- W2146819180 hasRelatedWork W2232132697 @default.
- W2146819180 hasRelatedWork W2987082249 @default.
- W2146819180 hasRelatedWork W3083532612 @default.
- W2146819180 hasRelatedWork W4207042721 @default.
- W2146819180 hasVolume "10" @default.
- W2146819180 isParatext "false" @default.
- W2146819180 isRetracted "false" @default.
- W2146819180 magId "2146819180" @default.
- W2146819180 workType "article" @default.