FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146886475> ?p ?o ?g. }

• W2146886475 endingPage "867" @default.
• W2146886475 startingPage "862" @default.
• W2146886475 abstract "SARS main protease is essential for life cycle of SARS coronavirus and may be a key target for developing anti-SARS drugs. Recently, the enzyme expressed in Escherichia coli was characterized using a HPLC assay to monitor the formation of products from 11 peptide substrates covering the cleavage sites found in the SARS viral genome. This protease easily dissociated into inactive monomer and the deduced Kd of the dimer was 100 microM. In order to detect enzyme activity, the assay needed to be performed at micromolar enzyme concentration. This makes finding the tight inhibitor (nanomolar range IC50) impossible. In this study, we prepared a peptide with fluorescence quenching pair (Dabcyl and Edans) at both ends of a peptide substrate and used this fluorogenic peptide substrate to characterize SARS main protease and screen inhibitors. The fluorogenic peptide gave extremely sensitive signal upon cleavage catalyzed by the protease. Using this substrate, the protease exhibits a significantly higher activity (kcat = 1.9 s(-1) and Km = 17 microM) compared to the previously reported parameters. Under our assay condition, the enzyme stays as an active dimer without dissociating into monomer and reveals a small Kd value (15 nM). This enzyme in conjunction with fluorogenic peptide substrate provides us a suitable tool for identifying potent inhibitors of SARS protease." @default.
• W2146886475 created "2016-06-24" @default.
• W2146886475 creator A5014668342 @default.
• W2146886475 creator A5019463541 @default.
• W2146886475 creator A5054555262 @default.
• W2146886475 creator A5058021944 @default.
• W2146886475 date "2004-06-01" @default.
• W2146886475 modified "2023-10-06" @default.
• W2146886475 title "Characterization of SARS main protease and inhibitor assay using a fluorogenic substrate" @default.
• W2146886475 cites W1966238900 @default.
• W2146886475 cites W1967669339 @default.
• W2146886475 cites W1976741900 @default.
• W2146886475 cites W1993829583 @default.
• W2146886475 cites W2014038821 @default.
• W2146886475 cites W2024832489 @default.
• W2146886475 cites W2025170735 @default.
• W2146886475 cites W2027484375 @default.
• W2146886475 cites W2028228093 @default.
• W2146886475 cites W2034740482 @default.
• W2146886475 cites W2057262668 @default.
• W2146886475 cites W2092093169 @default.
• W2146886475 cites W2104548316 @default.
• W2146886475 cites W2108018232 @default.
• W2146886475 cites W2116420909 @default.
• W2146886475 cites W2116586125 @default.
• W2146886475 cites W2126918460 @default.
• W2146886475 cites W2132260239 @default.
• W2146886475 cites W2135634425 @default.
• W2146886475 cites W2158812536 @default.
• W2146886475 cites W2169198329 @default.
• W2146886475 doi "https://doi.org/10.1016/j.bbrc.2004.04.098" @default.
• W2146886475 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7134607" @default.
• W2146886475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15147951" @default.
• W2146886475 hasPublicationYear "2004" @default.
• W2146886475 type Work @default.
• W2146886475 sameAs 2146886475 @default.
• W2146886475 citedByCount "147" @default.
• W2146886475 countsByYear W21468864752012 @default.
• W2146886475 countsByYear W21468864752013 @default.
• W2146886475 countsByYear W21468864752014 @default.
• W2146886475 countsByYear W21468864752015 @default.
• W2146886475 countsByYear W21468864752016 @default.
• W2146886475 countsByYear W21468864752017 @default.
• W2146886475 countsByYear W21468864752018 @default.
• W2146886475 countsByYear W21468864752019 @default.
• W2146886475 countsByYear W21468864752020 @default.
• W2146886475 countsByYear W21468864752021 @default.
• W2146886475 countsByYear W21468864752022 @default.
• W2146886475 countsByYear W21468864752023 @default.
• W2146886475 crossrefType "journal-article" @default.
• W2146886475 hasAuthorship W2146886475A5014668342 @default.
• W2146886475 hasAuthorship W2146886475A5019463541 @default.
• W2146886475 hasAuthorship W2146886475A5054555262 @default.
• W2146886475 hasAuthorship W2146886475A5058021944 @default.
• W2146886475 hasBestOaLocation W21468864751 @default.
• W2146886475 hasConcept C151730666 @default.
• W2146886475 hasConcept C175156509 @default.
• W2146886475 hasConcept C178790620 @default.
• W2146886475 hasConcept C181199279 @default.
• W2146886475 hasConcept C185592680 @default.
• W2146886475 hasConcept C18903297 @default.
• W2146886475 hasConcept C2776714187 @default.
• W2146886475 hasConcept C2777289219 @default.
• W2146886475 hasConcept C2779281246 @default.
• W2146886475 hasConcept C2779546866 @default.
• W2146886475 hasConcept C41183919 @default.
• W2146886475 hasConcept C43369102 @default.
• W2146886475 hasConcept C55493867 @default.
• W2146886475 hasConcept C56856141 @default.
• W2146886475 hasConcept C86803240 @default.
• W2146886475 hasConceptScore W2146886475C151730666 @default.
• W2146886475 hasConceptScore W2146886475C175156509 @default.
• W2146886475 hasConceptScore W2146886475C178790620 @default.
• W2146886475 hasConceptScore W2146886475C181199279 @default.
• W2146886475 hasConceptScore W2146886475C185592680 @default.
• W2146886475 hasConceptScore W2146886475C18903297 @default.
• W2146886475 hasConceptScore W2146886475C2776714187 @default.
• W2146886475 hasConceptScore W2146886475C2777289219 @default.
• W2146886475 hasConceptScore W2146886475C2779281246 @default.
• W2146886475 hasConceptScore W2146886475C2779546866 @default.
• W2146886475 hasConceptScore W2146886475C41183919 @default.
• W2146886475 hasConceptScore W2146886475C43369102 @default.
• W2146886475 hasConceptScore W2146886475C55493867 @default.
• W2146886475 hasConceptScore W2146886475C56856141 @default.
• W2146886475 hasConceptScore W2146886475C86803240 @default.
• W2146886475 hasIssue "4" @default.
• W2146886475 hasLocation W21468864751 @default.
• W2146886475 hasLocation W21468864752 @default.
• W2146886475 hasLocation W21468864753 @default.
• W2146886475 hasLocation W21468864754 @default.
• W2146886475 hasOpenAccess W2146886475 @default.
• W2146886475 hasPrimaryLocation W21468864751 @default.
• W2146886475 hasRelatedWork W1987375010 @default.
• W2146886475 hasRelatedWork W1998328106 @default.
• W2146886475 hasRelatedWork W2007717937 @default.
• W2146886475 hasRelatedWork W2017949075 @default.
• W2146886475 hasRelatedWork W2047434710 @default.
• W2146886475 hasRelatedWork W2109223608 @default.

• next