Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146916435> ?p ?o ?g. }
- W2146916435 endingPage "8074" @default.
- W2146916435 startingPage "8069" @default.
- W2146916435 abstract "Rolling circle amplification (RCA) has been widely used as an isothermal DNA amplification technique for diagnostic and bioanalytical applications. Because RCA involves repeated copying of the same circular DNA template by a DNA polymerase thousands of times, we hypothesized there exist DNA sequences that can function as optimal templates and produce more DNA amplicons within an allocated time. Herein we describe an in vitro selection effort conducted to search from a random sequence DNA pool for such templates for phi29 DNA polymerase, a frequently used polymerase for RCA. Diverse DNA molecules were isolated and they were characterized by richness in adenosine (A) and cytidine (C) nucleotides. The top ranked sequences exhibit superior RCA efficiency and the use of these templates for RCA results in significantly improved detection sensitivity. AC-rich sequences are expected to find useful applications for setting up effective RCA assays for biological sensing." @default.
- W2146916435 created "2016-06-24" @default.
- W2146916435 creator A5000460824 @default.
- W2146916435 creator A5006174346 @default.
- W2146916435 creator A5024055779 @default.
- W2146916435 creator A5046211673 @default.
- W2146916435 creator A5058689861 @default.
- W2146916435 creator A5070686308 @default.
- W2146916435 creator A5081367802 @default.
- W2146916435 date "2015-04-15" @default.
- W2146916435 modified "2023-10-07" @default.
- W2146916435 title "Optimal DNA Templates for Rolling Circle Amplification Revealed by In Vitro Selection" @default.
- W2146916435 cites W1520883967 @default.
- W2146916435 cites W1575228817 @default.
- W2146916435 cites W1964679272 @default.
- W2146916435 cites W1964858690 @default.
- W2146916435 cites W1974238889 @default.
- W2146916435 cites W1977709085 @default.
- W2146916435 cites W1978408159 @default.
- W2146916435 cites W1987565198 @default.
- W2146916435 cites W1989478468 @default.
- W2146916435 cites W1991872434 @default.
- W2146916435 cites W1995531133 @default.
- W2146916435 cites W2000499172 @default.
- W2146916435 cites W2004377350 @default.
- W2146916435 cites W2007826043 @default.
- W2146916435 cites W2008846361 @default.
- W2146916435 cites W2012442036 @default.
- W2146916435 cites W2014381990 @default.
- W2146916435 cites W2027001273 @default.
- W2146916435 cites W2030551376 @default.
- W2146916435 cites W2038311101 @default.
- W2146916435 cites W2041555260 @default.
- W2146916435 cites W2044006090 @default.
- W2146916435 cites W2047245967 @default.
- W2146916435 cites W2048398084 @default.
- W2146916435 cites W2050387685 @default.
- W2146916435 cites W2051373351 @default.
- W2146916435 cites W2053760406 @default.
- W2146916435 cites W2069688986 @default.
- W2146916435 cites W2072461007 @default.
- W2146916435 cites W2072609629 @default.
- W2146916435 cites W2073387419 @default.
- W2146916435 cites W2073775872 @default.
- W2146916435 cites W2074374189 @default.
- W2146916435 cites W2078670754 @default.
- W2146916435 cites W2079321730 @default.
- W2146916435 cites W2081325029 @default.
- W2146916435 cites W2090470897 @default.
- W2146916435 cites W2090867369 @default.
- W2146916435 cites W2093863716 @default.
- W2146916435 cites W2096300978 @default.
- W2146916435 cites W2096910687 @default.
- W2146916435 cites W2104092717 @default.
- W2146916435 cites W2104525153 @default.
- W2146916435 cites W2105915844 @default.
- W2146916435 cites W2110927099 @default.
- W2146916435 cites W2111046885 @default.
- W2146916435 cites W2115600226 @default.
- W2146916435 cites W2120172812 @default.
- W2146916435 cites W2125565427 @default.
- W2146916435 cites W2126361766 @default.
- W2146916435 cites W2126655283 @default.
- W2146916435 cites W2131629478 @default.
- W2146916435 cites W2132787939 @default.
- W2146916435 cites W2134409042 @default.
- W2146916435 cites W2142706826 @default.
- W2146916435 cites W2145109339 @default.
- W2146916435 cites W2151667123 @default.
- W2146916435 cites W2151982403 @default.
- W2146916435 cites W2161953634 @default.
- W2146916435 cites W2162461857 @default.
- W2146916435 cites W2168937674 @default.
- W2146916435 cites W2314348083 @default.
- W2146916435 cites W2315522702 @default.
- W2146916435 cites W2320281801 @default.
- W2146916435 cites W2324905789 @default.
- W2146916435 cites W2332019778 @default.
- W2146916435 cites W4232155583 @default.
- W2146916435 cites W4232939329 @default.
- W2146916435 cites W4240583130 @default.
- W2146916435 cites W4247249653 @default.
- W2146916435 cites W4248892641 @default.
- W2146916435 cites W4251347853 @default.
- W2146916435 cites W4376453500 @default.
- W2146916435 doi "https://doi.org/10.1002/chem.201500994" @default.
- W2146916435 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25877998" @default.
- W2146916435 hasPublicationYear "2015" @default.
- W2146916435 type Work @default.
- W2146916435 sameAs 2146916435 @default.
- W2146916435 citedByCount "26" @default.
- W2146916435 countsByYear W21469164352015 @default.
- W2146916435 countsByYear W21469164352016 @default.
- W2146916435 countsByYear W21469164352017 @default.
- W2146916435 countsByYear W21469164352018 @default.
- W2146916435 countsByYear W21469164352020 @default.
- W2146916435 countsByYear W21469164352021 @default.
- W2146916435 countsByYear W21469164352022 @default.