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- W2146969759 abstract "Abstract A rapid and effective lateral flow assay (LFA) for detection of avian influenza virus (AIV) was developed. For antigen capture, the assay used monoclonal antibody specific for a conserved nuclear protein (NP) epitope, immobilized on a cellulose acetate matrix, in conjunction with a second NP monoclonal antibody chemically linked to either coloured latex beads or colloidal gold particles contained in a sample pad for detection. Virus sample added to the sample pad flowed into the trapping antibody to form a visible band as well as a second, control band further along the acetate strip. The control band consisted of recombinant protein A/G, also immobilized on the matrix. A second LFA for detection of chicken antibody to AIV was developed where NP antigen was immobilized on the matrix with recombinant protein A/G immobilized as a control band. Latex beads or colloidal gold particles to which monoclonal anti‐chicken antibody was attached, were used as the indicator system." @default.
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- W2146969759 date "2007-09-01" @default.
- W2146969759 modified "2023-09-27" @default.
- W2146969759 title "Prototype Single Step Lateral Flow Technology for Detection of Avian Influenza Virus and Chicken Antibody to Avian Influenza Virus" @default.
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- W2146969759 doi "https://doi.org/10.1080/15321810701603443" @default.
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