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• W2146998517 endingPage "373" @default.
• W2146998517 startingPage "335" @default.
• W2146998517 abstract "One of the most problematic groups of the USEPA and EU priority pollutants are the halogenated organic compounds. These substances have a wide range of industrial applications, such as solvents and cleaners. Inadequate disposal techniques and accidental spillages have led to their detection in soil, groundwater, and river sediments. Persistence of these compounds in the environment has resulted from low levels of biodegradation due to chemical structural features that preclude or retard biological attack. Research has indicated the idea that treatment systems based on methanotrophic co-metabolic transformation may be a cost-effective and efficient alternative to physical methods because of the potential for high transformation rates, the possibility of complete compound degradation without the formation of toxic metabolites, applicability to a broad spectrum of compounds, and the use of a widely available and inexpensive growth substrate. A substantial amount of work concerning methanotrophic cometabolic transformations has been carried out using the soluble form of methane monooxygenase (sMMO) from the obligate methanotroph Methylosinus trichosporium OB3b. This NADH-dependent monooxygenase is derepressed when cells are grown under copper stress. sMMO has a wider specificity than the particulate form. sMMO has been shown to degrade trichloroethylene (TCE) at a rate of at least one order of magnitude faster than obtained with other mixed and pure cultures, suggesting it has a wider application to bioremediation. Furthermore, sMMO catalyzes an unusually wide range of oxidation reactions, including the hydroxylation of alkanes, epoxidation of alkenes, ethers, halogenated methanes, cyclic and aromatic compounds including compounds, that are resistant to degradation in the environment.However, the practical application of methantrophs and Methylosinus trichosporium OB3b to the treatment of chlorinated organics has met with mixed success. Although oxidation rates are rapid, compound oxidation with M. trichosporium OB3b is difficult. This fastidious organism grows relatively slowly, which limits the speed with which sMMO expressing biomass can be generated. Furthermore, product toxicity toward the cell, affecting the stability of the enzyme when transforming certain compounds has been observed, for example, by the products of 1,2,3 trichlorobenzene hydroxylation (2,3,4- and 3,4,5-trichlorophenol) and of TCE degradation (chloral hydrate). Because of this toxicity and the inability of sMMO to further oxidize its own hydroxylation products, the ability of methane monoxygenase to carry out the monooxygenation of a wide variety of substituted aromatics and polyaromatics cannot be fully exploited in M. trichosporium OB3b.Many of these problems could be overcome by the use of either a mixed downstream heterotrophic population of organisms that could accommodate the products of hydroxylation or to express sMMO in an organism that could metabolize the products of hydroxylation. The latter of these two approaches would have several advantages. The main benefit would be the removal of the need for methane, which is required to induce sMMO in M. trichosporium OB3b, and supply carbon and energy to the cells that continuously oxidise the target compound, but also acts as a competitive inhibitor of sMMO. Instead, the recombinant could utilize the products of sMMO-mediated hydroxylation as a carbon source." @default.
• W2146998517 created "2016-06-24" @default.
• W2146998517 creator A5023258913 @default.
• W2146998517 creator A5030698014 @default.
• W2146998517 creator A5044931603 @default.
• W2146998517 date "1998-01-01" @default.
• W2146998517 modified "2023-09-26" @default.
• W2146998517 title "Methanotrophs,<i>Methylosinus trichosporium</i>OB3b, sMMO, and Their Application to Bioremediation" @default.
• W2146998517 cites W1479683065 @default.
• W2146998517 cites W1484229853 @default.
• W2146998517 cites W1493511953 @default.
• W2146998517 cites W1493719049 @default.
• W2146998517 cites W1506106735 @default.
• W2146998517 cites W1510751198 @default.
• W2146998517 cites W1517943446 @default.
• W2146998517 cites W1518243624 @default.
• W2146998517 cites W1520975302 @default.
• W2146998517 cites W1531121676 @default.
• W2146998517 cites W1539429836 @default.
• W2146998517 cites W1544105047 @default.
• W2146998517 cites W1547072346 @default.
• W2146998517 cites W1550122207 @default.
• W2146998517 cites W1551814984 @default.
• W2146998517 cites W1567116460 @default.
• W2146998517 cites W1574473232 @default.
• W2146998517 cites W157582913 @default.
• W2146998517 cites W157793871 @default.
• W2146998517 cites W1587187748 @default.
• W2146998517 cites W1590302505 @default.
• W2146998517 cites W1590365266 @default.
• W2146998517 cites W1590883629 @default.
• W2146998517 cites W1602201841 @default.
• W2146998517 cites W1603280106 @default.
• W2146998517 cites W1607928695 @default.
• W2146998517 cites W1695135775 @default.
• W2146998517 cites W1822040134 @default.
• W2146998517 cites W182249958 @default.
• W2146998517 cites W1847764357 @default.
• W2146998517 cites W1909714633 @default.
• W2146998517 cites W1922688230 @default.
• W2146998517 cites W1964803960 @default.
• W2146998517 cites W1968366930 @default.
• W2146998517 cites W1972212413 @default.
• W2146998517 cites W1979632844 @default.
• W2146998517 cites W1984951489 @default.
• W2146998517 cites W1986035139 @default.
• W2146998517 cites W1989122605 @default.
• W2146998517 cites W1989433823 @default.
• W2146998517 cites W1995450972 @default.
• W2146998517 cites W1996154772 @default.
• W2146998517 cites W2003408264 @default.
• W2146998517 cites W2004962085 @default.
• W2146998517 cites W2006035277 @default.
• W2146998517 cites W2010160430 @default.
• W2146998517 cites W2011225358 @default.
• W2146998517 cites W2011607516 @default.
• W2146998517 cites W2016809780 @default.
• W2146998517 cites W2017035688 @default.
• W2146998517 cites W2018068798 @default.
• W2146998517 cites W2024010385 @default.
• W2146998517 cites W2025337090 @default.
• W2146998517 cites W2025769988 @default.
• W2146998517 cites W2029546445 @default.
• W2146998517 cites W2034045575 @default.
• W2146998517 cites W2038757997 @default.
• W2146998517 cites W2039698839 @default.
• W2146998517 cites W2039823965 @default.
• W2146998517 cites W2040372215 @default.
• W2146998517 cites W2040965892 @default.
• W2146998517 cites W2042703730 @default.
• W2146998517 cites W2050631703 @default.
• W2146998517 cites W2051245368 @default.
• W2146998517 cites W2056785088 @default.
• W2146998517 cites W2061415354 @default.
• W2146998517 cites W2062024634 @default.
• W2146998517 cites W2063904689 @default.
• W2146998517 cites W2065607402 @default.
• W2146998517 cites W2068344403 @default.
• W2146998517 cites W2068431139 @default.
• W2146998517 cites W2069143970 @default.
• W2146998517 cites W2073466318 @default.
• W2146998517 cites W2073725406 @default.
• W2146998517 cites W2075302555 @default.
• W2146998517 cites W2075621094 @default.
• W2146998517 cites W2075979069 @default.
• W2146998517 cites W2076506442 @default.
• W2146998517 cites W2080456846 @default.
• W2146998517 cites W2084770761 @default.
• W2146998517 cites W2087531418 @default.
• W2146998517 cites W2089850604 @default.
• W2146998517 cites W2091132856 @default.
• W2146998517 cites W2092962150 @default.
• W2146998517 cites W2093249405 @default.
• W2146998517 cites W2095402750 @default.
• W2146998517 cites W2099998333 @default.
• W2146998517 cites W2101963042 @default.
• W2146998517 cites W2104041344 @default.
• W2146998517 cites W2110980311 @default.

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