FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2147130249> ?p ?o ?g. }

• W2147130249 endingPage "L1020" @default.
• W2147130249 startingPage "L1012" @default.
• W2147130249 abstract "We previously reported that hypoxia attenuates cGMP-dependent protein kinase (PKG)-mediated relaxation in pulmonary vessels (Am J Physiol Lung Cell Mol Physiol 279: L611-L618, 2003). To determine whether hypoxia-induced reactive oxygen and nitrogen species (ROS and RNS, respectively) may be involved in the downregulation of PKG-mediated relaxation, ovine fetal intrapulmonary veins were exposed to 4 h of normoxia or hypoxia, with or without scavengers of ROS [N-acetylcysteine (NAC)] or peroxynitrite (quercetin and Trolox) and preconstricted with endothelin-1. Hypoxia decreased the relaxation response to 8-bromo-cGMP, PKG protein expression, and kinase activity and increased tyrosine nitration in PKG. However, ROS and RNS scavengers prevented these changes. To determine whether increased PKG nitration diminishes PKG activity, pulmonary vein smooth muscle cells (PVSMC) were exposed to shorter-term (30 min) hypoxia, which increased PKG nitration and decreased PKG activity but did not alter PKG protein expression. Increased dihydro-2,7-dichlorofluorescein diacetate (DCFH(2)-DA) fluorescence in PVSMC after 4 h or 30 min of hypoxia was not observed in the presence of NAC, quercetin, or Trolox, suggesting increased ROS and RNS production. Increased PKG nitration and the associated decrease in PKG activity in PVSMC after 30 min of hypoxia were also reversed on reoxygenation. The consequences of PKG nitration were assessed by exposure of purified PKG-Ialpha to peroxynitrite, which caused increased 3-nitrotyrosine immunoreactivity and inhibition of kinase activity. Our data suggest that, after 30 min of hypoxia, reversible covalent modification of PKG by hypoxia-induced reactive species may be an important mechanism by which the relaxation response to cGMP is regulated. However, after 4 h of hypoxia, PKG nitration and decreased PKG expression are involved." @default.
• W2147130249 created "2016-06-24" @default.
• W2147130249 creator A5006657438 @default.
• W2147130249 creator A5024988369 @default.
• W2147130249 creator A5067972776 @default.
• W2147130249 creator A5072263132 @default.
• W2147130249 creator A5072994758 @default.
• W2147130249 creator A5079194052 @default.
• W2147130249 date "2007-10-01" @default.
• W2147130249 modified "2023-10-15" @default.
• W2147130249 title "Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins" @default.
• W2147130249 cites W1250641235 @default.
• W2147130249 cites W133579554 @default.
• W2147130249 cites W1505538037 @default.
• W2147130249 cites W1523151859 @default.
• W2147130249 cites W1543601456 @default.
• W2147130249 cites W1582753616 @default.
• W2147130249 cites W1601111461 @default.
• W2147130249 cites W1821918060 @default.
• W2147130249 cites W1850731934 @default.
• W2147130249 cites W1964990390 @default.
• W2147130249 cites W1969489400 @default.
• W2147130249 cites W1976652573 @default.
• W2147130249 cites W1980528903 @default.
• W2147130249 cites W1981502718 @default.
• W2147130249 cites W1983372621 @default.
• W2147130249 cites W1986703419 @default.
• W2147130249 cites W1987690324 @default.
• W2147130249 cites W1990510961 @default.
• W2147130249 cites W1997469875 @default.
• W2147130249 cites W2000075945 @default.
• W2147130249 cites W2007354513 @default.
• W2147130249 cites W2017733030 @default.
• W2147130249 cites W2018101787 @default.
• W2147130249 cites W2019738475 @default.
• W2147130249 cites W2020276494 @default.
• W2147130249 cites W2020600203 @default.
• W2147130249 cites W2027439180 @default.
• W2147130249 cites W2028725139 @default.
• W2147130249 cites W2034832260 @default.
• W2147130249 cites W2042853579 @default.
• W2147130249 cites W2046798893 @default.
• W2147130249 cites W2050917247 @default.
• W2147130249 cites W2051809818 @default.
• W2147130249 cites W2052327619 @default.
• W2147130249 cites W2060243949 @default.
• W2147130249 cites W2089461760 @default.
• W2147130249 cites W2093382413 @default.
• W2147130249 cites W2094213529 @default.
• W2147130249 cites W2103221376 @default.
• W2147130249 cites W2104789917 @default.
• W2147130249 cites W2114011224 @default.
• W2147130249 cites W2125141239 @default.
• W2147130249 cites W2127162829 @default.
• W2147130249 cites W2128022125 @default.
• W2147130249 cites W2129307432 @default.
• W2147130249 cites W2130400938 @default.
• W2147130249 cites W2131947494 @default.
• W2147130249 cites W2132704929 @default.
• W2147130249 cites W2140857653 @default.
• W2147130249 cites W2146691809 @default.
• W2147130249 cites W2158802782 @default.
• W2147130249 cites W2163917074 @default.
• W2147130249 cites W2165178433 @default.
• W2147130249 cites W2189297449 @default.
• W2147130249 cites W2281664524 @default.
• W2147130249 cites W2335875126 @default.
• W2147130249 cites W2406750524 @default.
• W2147130249 cites W2413327079 @default.
• W2147130249 cites W2425180512 @default.
• W2147130249 cites W4241495685 @default.
• W2147130249 doi "https://doi.org/10.1152/ajplung.00061.2007" @default.
• W2147130249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17616649" @default.
• W2147130249 hasPublicationYear "2007" @default.
• W2147130249 type Work @default.
• W2147130249 sameAs 2147130249 @default.
• W2147130249 citedByCount "33" @default.
• W2147130249 countsByYear W21471302492012 @default.
• W2147130249 countsByYear W21471302492013 @default.
• W2147130249 countsByYear W21471302492014 @default.
• W2147130249 countsByYear W21471302492015 @default.
• W2147130249 countsByYear W21471302492016 @default.
• W2147130249 countsByYear W21471302492017 @default.
• W2147130249 countsByYear W21471302492018 @default.
• W2147130249 countsByYear W21471302492019 @default.
• W2147130249 countsByYear W21471302492023 @default.
• W2147130249 crossrefType "journal-article" @default.
• W2147130249 hasAuthorship W2147130249A5006657438 @default.
• W2147130249 hasAuthorship W2147130249A5024988369 @default.
• W2147130249 hasAuthorship W2147130249A5067972776 @default.
• W2147130249 hasAuthorship W2147130249A5072263132 @default.
• W2147130249 hasAuthorship W2147130249A5072994758 @default.
• W2147130249 hasAuthorship W2147130249A5079194052 @default.
• W2147130249 hasConcept C126322002 @default.
• W2147130249 hasConcept C134018914 @default.
• W2147130249 hasConcept C173803235 @default.
• W2147130249 hasConcept C178790620 @default.
• W2147130249 hasConcept C181199279 @default.

• next