Matches in SemOpenAlex for { <https://semopenalex.org/work/W2147278052> ?p ?o ?g. }
- W2147278052 endingPage "5740" @default.
- W2147278052 startingPage "5733" @default.
- W2147278052 abstract "ABSTRACT Cytotoxic necrotizing factor type 1 (CNF1) from Escherichia coli activates the small GTP-binding proteins of the Rho family (Rho, Rac, and Cdc42) by catalyzing their deamidation at a specific glutamine residue. Since RhoA, Rac, and Cdc42 play a pivotal role in cell migration during the early phase of wound repair, we investigated whether CNF1 was able to interfere with wound healing in intestinal epithelial monolayers (T84 cells). After mechanical injury, we found that CNF1 blocks epithelial wound repair within 48 h. This effect was characterized by cell elongation and filopodium formation on the leading edge, in association with permanent phosphorylation of the focal adhesion kinase (FAK) via Rho activation. Moreover, inhibition of Rho kinase with Y-27632 decreased CNF1-mediated permanent FAK phosphorylation, leading to complete restitution of wound repair within 24 h. In addition, we found that CNF1 induced upregulation of mitogen-activated protein kinases (MAPK) activation. Moreover, activation of Rac and MAPK by CNF1 increased matrix metalloproteinase 9 expression in wounded T84 monolayers. Taken together, these results provide evidence that CNF1 strongly impairs intestinal epithelial wound healing." @default.
- W2147278052 created "2016-06-24" @default.
- W2147278052 creator A5009645818 @default.
- W2147278052 creator A5011103654 @default.
- W2147278052 creator A5019109478 @default.
- W2147278052 creator A5019765162 @default.
- W2147278052 creator A5028395175 @default.
- W2147278052 creator A5038889836 @default.
- W2147278052 creator A5075027068 @default.
- W2147278052 creator A5089171072 @default.
- W2147278052 date "2004-10-01" @default.
- W2147278052 modified "2023-10-18" @default.
- W2147278052 title "<i>Escherichia coli</i> Cytotoxic Necrotizing Factor 1 Inhibits Intestinal Epithelial Wound Healing In Vitro after Mechanical Injury" @default.
- W2147278052 cites W129191972 @default.
- W2147278052 cites W1495826882 @default.
- W2147278052 cites W1606615500 @default.
- W2147278052 cites W1623264536 @default.
- W2147278052 cites W1647404354 @default.
- W2147278052 cites W1822966053 @default.
- W2147278052 cites W1958495494 @default.
- W2147278052 cites W1960735136 @default.
- W2147278052 cites W1968826282 @default.
- W2147278052 cites W1974245054 @default.
- W2147278052 cites W1977127210 @default.
- W2147278052 cites W1990580084 @default.
- W2147278052 cites W1995757732 @default.
- W2147278052 cites W2001232517 @default.
- W2147278052 cites W2002827724 @default.
- W2147278052 cites W2007660746 @default.
- W2147278052 cites W2021683914 @default.
- W2147278052 cites W2021944416 @default.
- W2147278052 cites W2025437109 @default.
- W2147278052 cites W2027152233 @default.
- W2147278052 cites W2027810531 @default.
- W2147278052 cites W2028691987 @default.
- W2147278052 cites W2036145275 @default.
- W2147278052 cites W2039418057 @default.
- W2147278052 cites W2044237810 @default.
- W2147278052 cites W2049218641 @default.
- W2147278052 cites W2050087493 @default.
- W2147278052 cites W2054210804 @default.
- W2147278052 cites W2062023732 @default.
- W2147278052 cites W2063538689 @default.
- W2147278052 cites W2090011997 @default.
- W2147278052 cites W2093089107 @default.
- W2147278052 cites W2093226717 @default.
- W2147278052 cites W2093629370 @default.
- W2147278052 cites W2095268506 @default.
- W2147278052 cites W2096791952 @default.
- W2147278052 cites W2096915545 @default.
- W2147278052 cites W2105791301 @default.
- W2147278052 cites W2109952947 @default.
- W2147278052 cites W2116798763 @default.
- W2147278052 cites W2118471853 @default.
- W2147278052 cites W2118633267 @default.
- W2147278052 cites W2119435197 @default.
- W2147278052 cites W2122381944 @default.
- W2147278052 cites W2128049766 @default.
- W2147278052 cites W2130838656 @default.
- W2147278052 cites W2142042013 @default.
- W2147278052 cites W2152184242 @default.
- W2147278052 cites W2154936502 @default.
- W2147278052 cites W2162049443 @default.
- W2147278052 cites W2181898446 @default.
- W2147278052 cites W2183019695 @default.
- W2147278052 cites W2322155361 @default.
- W2147278052 cites W2325727928 @default.
- W2147278052 doi "https://doi.org/10.1128/iai.72.10.5733-5740.2004" @default.
- W2147278052 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/517525" @default.
- W2147278052 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15385472" @default.
- W2147278052 hasPublicationYear "2004" @default.
- W2147278052 type Work @default.
- W2147278052 sameAs 2147278052 @default.
- W2147278052 citedByCount "11" @default.
- W2147278052 countsByYear W21472780522020 @default.
- W2147278052 countsByYear W21472780522021 @default.
- W2147278052 countsByYear W21472780522022 @default.
- W2147278052 crossrefType "journal-article" @default.
- W2147278052 hasAuthorship W2147278052A5009645818 @default.
- W2147278052 hasAuthorship W2147278052A5011103654 @default.
- W2147278052 hasAuthorship W2147278052A5019109478 @default.
- W2147278052 hasAuthorship W2147278052A5019765162 @default.
- W2147278052 hasAuthorship W2147278052A5028395175 @default.
- W2147278052 hasAuthorship W2147278052A5038889836 @default.
- W2147278052 hasAuthorship W2147278052A5075027068 @default.
- W2147278052 hasAuthorship W2147278052A5089171072 @default.
- W2147278052 hasBestOaLocation W21472780521 @default.
- W2147278052 hasConcept C11960822 @default.
- W2147278052 hasConcept C151166631 @default.
- W2147278052 hasConcept C184235292 @default.
- W2147278052 hasConcept C203014093 @default.
- W2147278052 hasConcept C2777093181 @default.
- W2147278052 hasConcept C2780040266 @default.
- W2147278052 hasConcept C2780269544 @default.
- W2147278052 hasConcept C57074206 @default.
- W2147278052 hasConcept C62478195 @default.
- W2147278052 hasConcept C86803240 @default.
- W2147278052 hasConcept C95444343 @default.