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• W2147375303 abstract "Bacillus anthracis begins its infectious cycle as a metabolically dormant cell type, the endospore. Upon entry into a host, endospores rapidly differentiate into vegetative bacilli through the process of germination, thus initiating anthrax. Elucidation of the signals that trigger germination and the receptors that recognize them is critical to understanding the pathogenesis of B. anthracis. Individual mutants deficient in each of the seven putative germinant receptor-encoding loci were constructed via temperature-dependent, plasmid insertion mutagenesis and used to correlate these receptors with known germinant molecules. These analyses showed that the GerK and GerL receptors are jointly required for the alanine germination pathway and also are individually required for recognition of either proline and methionine (GerK) or serine and valine (GerL) as cogerminants in combination with inosine. The germinant specificity of GerS was refined from a previous study in a nonisogenic background since it was required only for germination in response to aromatic amino acid cogerminants. The gerA and gerY loci were found to be dispensable for recognition of all known germinant molecules. In addition, we show that the promoter of each putative germinant receptor operon, except that of the gerA locus, is active during sporulation. A current model of B. anthracis endospore germination is presented." @default.
• W2147375303 created "2016-06-24" @default.
• W2147375303 creator A5025023534 @default.
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• W2147375303 date "2005-12-01" @default.
• W2147375303 modified "2023-10-01" @default.
• W2147375303 title "Characterization of <i>Bacillus anthracis</i> Germinant Receptors In Vitro" @default.
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• W2147375303 doi "https://doi.org/10.1128/jb.187.23.8055-8062.2005" @default.
• W2147375303 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1291278" @default.
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