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• W2147524887 abstract "Recombinant Escherichia coli whole cells harboring Bacillus licheniformis L-arabinose isomerase (BLAI) were harvested to prepare alginate-immobilized biocatalysts. The operational conditions for immobilization were optimized according to relative activity and the cell leakage of the immobilized cell. The optimal conditions are as follows: alginate concentration, Ca(2+) concentration, cell mass loading, and curing time were 2% (w/v), 0.1 M, 50 g l(-1), and 4 hours, respectively. After immobilization, cross-linking with 0.1% glutaraldehyde significantly reduced cell leakage. The immobilized whole cells harboring BLAI were very stable with 89% residual activity remaining after 33 days of incubation at 50 degrees C and were much more stable than the free enzyme and cells. The results showed that immobilizing whole cells harboring BLAI is suitable for use as a biocatalyst in the production of L-ribulose, largely due to its high stability and low cost." @default.
• W2147524887 created "2016-06-24" @default.
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• W2147524887 date "2009-12-14" @default.
• W2147524887 modified "2023-10-18" @default.
• W2147524887 title "ENHANCED STABILITY OFBACILLUS LICHENIFORMISL-ARABINOSE ISOMERASE BY IMMOBILIZATION WITH ALGINATE" @default.
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• W2147524887 doi "https://doi.org/10.1080/10826060903400567" @default.
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