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• W2147553026 startingPage "1093" @default.
• W2147553026 abstract "Both insulin and IGF–1 receptors are present in intestinal mucosal cells, although their role in this tissue is unclear. We have characterized these receptors in a human adenocarcinoma cell line, Caco-2, and examined their role in the regulation of glucose transport and absorption in these cells. The Caco-2 cells demonstrated specific insulin and IGF-1 receptors. They also bound cytochalasin B, suggesting the presence of a glucose transporter-like protein. When grown on membranes, the Caco-2 cells formed columnar, bipolar cells with tight junctions. The monolayer selectively transported D-glucose, and methyl-D-glucose, with complete exclusion of L-glucose, D-mannitol and inulin. The absorption of glucose across the monolayer occurred via a Na+/glucose cotransporter, as indicated by a change in short circuit current after addition of glucose to the apical membrane. When examined under several conditions, neither insulin nor IGF-1 had an affect on the transport of glucose across the Caco-2 monolayer, nor the production of lactate by the cells. It is concluded that the insulin and IGF-1 receptors of Caco-2 cells do not regulate glucose transport." @default.
• W2147553026 created "2016-06-24" @default.
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• W2147553026 date "1993-01-01" @default.
• W2147553026 modified "2023-10-15" @default.
• W2147553026 title "Insulin and IGF-1 Receptors in A Human Intestinal Adenocarcinoma Cell Line (Caco-2): Regulation of Na⁺Glucose Transport Across the Brush Border" @default.
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• W2147553026 doi "https://doi.org/10.3109/10799899309063266" @default.
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