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• W2147558603 endingPage "1771" @default.
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• W2147558603 abstract "Abstract. Abnormal O -glycosylation of IgA1 may contribute to pathogenic mechanisms in IgA nephropathy (IgAN). Observations of altered lectin binding to IgA1 in IgAN suggest that the O -glycan chains may be undergalactosylated, but precise structural definition of the defect has proved technically difficult, and it remains unconfirmed. This is the first study using fluorophore-assisted carbohydrate electrophoresis (FACE) to analyze IgA1 O -glycans in IgAN and controls. IgA1 was purified from serum, and the intact O -glycans were released by hydrazinolysis at 60°C. After re- N -acetylation, the glycans were fluorophore-labeled and separated by polyacrylamide gel electrophoresis. Sequential exoglycosidase digestions of IgA1 allowed identification of the different O -glycan bands on FACE gels, and their relative frequencies in IgA1 samples were measured by ultraviolet densitometry. Lectin binding of the IgA1 samples was also measured. In some patients with IgAN, FACE analysis demonstrated a significant increase in the percentage of IgA1 O -glycan chains consisting of single N -acetyl galactosamine (GalNAc) units rather than the more usual galactosylated and sialylated forms. This finding was confirmed using both desialylated IgA1 and enzymatically released O -glycans. Good correlation was also found between O -glycan agalactosylation on FACE analysis and IgA1 lectin binding in IgAN, supporting the value of lectins as tools for detection of this abnormality. This is the first study in which all of the predicted O -glycan forms of IgA1 have been analyzed simultaneously, and demonstrates that in IgAN, the IgA1 O -glycan chains are truncated, with increased terminal GalNAc. This abnormality has the potential to significantly affect IgA1 behavior and handling with pathogenic consequences in IgAN." @default.
• W2147558603 created "2016-06-24" @default.
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• W2147558603 date "1999-08-01" @default.
• W2147558603 modified "2023-10-17" @default.
• W2147558603 title "Analysis of IgA1 O-Glycans in IgA Nephropathy by Fluorophore-Assisted Carbohydrate Electrophoresis" @default.
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• W2147558603 doi "https://doi.org/10.1681/asn.v1081763" @default.
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