Matches in SemOpenAlex for { <https://semopenalex.org/work/W2147634355> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2147634355 endingPage "185" @default.
- W2147634355 startingPage "173" @default.
- W2147634355 abstract "SUMMARY: When concentrated by precipitation with acid and salts, or by highspeed centrifugation, potato virus X tends to become insoluble though still remaining infective and serologically active. This greatly complicates purification and no method was found that could be relied upon to give good yields of virus with constant properties. Insolubility is correlated with the aggregation of virus particles to form long threads that become entangled, but it is probable that combination of the particles with some cell constituents is also concerned. Insoluble preparations dissolve slowly when incubated with pH 7·5 borate buffer, and rapidly in the presence of trypsin or chymotrypsin. Both of these enzymes hydrolyse virus X, chymotrypsin being the more effective, but different strains of the virus vary in their susceptibility. Ribonuclease readily hydrolyses the nucleic acid derived from virus X, but seems to have no enzymic action on the active virus. When mixed with the virus, the enzyme combines with it and reversibly inhibits infectivity. At pH 7 the addition of ribonuclease to soluble virus preparations causes loss of anisotropy of flow, a fall in precipitin titre, and the production of an insoluble complex. Incubation at pH 7·5 with borate buffer slowly dissolves the complex and restores the original properties of the virus; the rate of re-solution is increased by the presence of trypsin. Some preparations of the virus were partially decomposed by incubation with borate buffer, and sometimes the rate of decomposition was increased in the presence of ribonuclease." @default.
- W2147634355 created "2016-06-24" @default.
- W2147634355 creator A5065213097 @default.
- W2147634355 creator A5087136984 @default.
- W2147634355 date "1948-05-01" @default.
- W2147634355 modified "2023-09-25" @default.
- W2147634355 title "Variations in the Properties of Potato Virus X and their Effects on its Interactions with Ribonuclease and Proteolytic Enzymes" @default.
- W2147634355 cites W191192900 @default.
- W2147634355 cites W1980531366 @default.
- W2147634355 cites W2009001017 @default.
- W2147634355 cites W2048315728 @default.
- W2147634355 cites W2254425217 @default.
- W2147634355 cites W2257388444 @default.
- W2147634355 cites W2344000682 @default.
- W2147634355 cites W2397011887 @default.
- W2147634355 cites W747415324 @default.
- W2147634355 cites W867801306 @default.
- W2147634355 doi "https://doi.org/10.1099/00221287-2-2-173" @default.
- W2147634355 hasPublicationYear "1948" @default.
- W2147634355 type Work @default.
- W2147634355 sameAs 2147634355 @default.
- W2147634355 citedByCount "22" @default.
- W2147634355 crossrefType "journal-article" @default.
- W2147634355 hasAuthorship W2147634355A5065213097 @default.
- W2147634355 hasAuthorship W2147634355A5087136984 @default.
- W2147634355 hasBestOaLocation W21476343551 @default.
- W2147634355 hasConcept C104317684 @default.
- W2147634355 hasConcept C106358424 @default.
- W2147634355 hasConcept C10879258 @default.
- W2147634355 hasConcept C133229898 @default.
- W2147634355 hasConcept C147483822 @default.
- W2147634355 hasConcept C159047783 @default.
- W2147634355 hasConcept C181199279 @default.
- W2147634355 hasConcept C185592680 @default.
- W2147634355 hasConcept C2522874641 @default.
- W2147634355 hasConcept C25642318 @default.
- W2147634355 hasConcept C2778401398 @default.
- W2147634355 hasConcept C2778976237 @default.
- W2147634355 hasConcept C2780340462 @default.
- W2147634355 hasConcept C2780704907 @default.
- W2147634355 hasConcept C43617362 @default.
- W2147634355 hasConcept C54355233 @default.
- W2147634355 hasConcept C55493867 @default.
- W2147634355 hasConcept C67705224 @default.
- W2147634355 hasConcept C7623868 @default.
- W2147634355 hasConcept C86803240 @default.
- W2147634355 hasConcept C94412978 @default.
- W2147634355 hasConceptScore W2147634355C104317684 @default.
- W2147634355 hasConceptScore W2147634355C106358424 @default.
- W2147634355 hasConceptScore W2147634355C10879258 @default.
- W2147634355 hasConceptScore W2147634355C133229898 @default.
- W2147634355 hasConceptScore W2147634355C147483822 @default.
- W2147634355 hasConceptScore W2147634355C159047783 @default.
- W2147634355 hasConceptScore W2147634355C181199279 @default.
- W2147634355 hasConceptScore W2147634355C185592680 @default.
- W2147634355 hasConceptScore W2147634355C2522874641 @default.
- W2147634355 hasConceptScore W2147634355C25642318 @default.
- W2147634355 hasConceptScore W2147634355C2778401398 @default.
- W2147634355 hasConceptScore W2147634355C2778976237 @default.
- W2147634355 hasConceptScore W2147634355C2780340462 @default.
- W2147634355 hasConceptScore W2147634355C2780704907 @default.
- W2147634355 hasConceptScore W2147634355C43617362 @default.
- W2147634355 hasConceptScore W2147634355C54355233 @default.
- W2147634355 hasConceptScore W2147634355C55493867 @default.
- W2147634355 hasConceptScore W2147634355C67705224 @default.
- W2147634355 hasConceptScore W2147634355C7623868 @default.
- W2147634355 hasConceptScore W2147634355C86803240 @default.
- W2147634355 hasConceptScore W2147634355C94412978 @default.
- W2147634355 hasIssue "2" @default.
- W2147634355 hasLocation W21476343551 @default.
- W2147634355 hasOpenAccess W2147634355 @default.
- W2147634355 hasPrimaryLocation W21476343551 @default.
- W2147634355 hasRelatedWork W1924000961 @default.
- W2147634355 hasRelatedWork W1984310206 @default.
- W2147634355 hasRelatedWork W1994138835 @default.
- W2147634355 hasRelatedWork W2033935988 @default.
- W2147634355 hasRelatedWork W2054787098 @default.
- W2147634355 hasRelatedWork W2063845125 @default.
- W2147634355 hasRelatedWork W2147634355 @default.
- W2147634355 hasRelatedWork W2410856592 @default.
- W2147634355 hasRelatedWork W2775212963 @default.
- W2147634355 hasRelatedWork W973827038 @default.
- W2147634355 hasVolume "2" @default.
- W2147634355 isParatext "false" @default.
- W2147634355 isRetracted "false" @default.
- W2147634355 magId "2147634355" @default.
- W2147634355 workType "article" @default.