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• W2147918084 abstract "Recombination was studied in λ DNA half molecules employing the helper infectivity assay for free DNA. The assay permits examination of the effects of both gene mutation and physical modification for the λ genome on site-specific (Int) and general recombination (Red, Rec). Each half molecule bears a single cohesive end, in contrast to whole molecules which bear two, and is therefore unable to circularize. Recombination was measured between helper DNA (whole molecules) and infecting DNA (whole or half molecules). Whole DNA molecules take part, independently, in both Int and Red promoted recombination. Left half molecules do not participate in recombination. Right half molecules display Red promoted recombination but are totally defective in productive site-specific recombination. The defect in right half molecules responsible for this behavior could not be attributed to physical damage at the int gene or att site during their preparation. The defect could be remedied by providing general recombination functions, so that a novel coupling of the Int and Rec systems led to productive site-specific recombination in right half molecules. The results indicate that λ DNA requires both cohesive ends in order to engage in productive site-specific recombination. This is interpreted to show a requirement for circularity in λ growth." @default.
• W2147918084 created "2016-06-24" @default.
• W2147918084 creator A5080274243 @default.
• W2147918084 date "1972-04-01" @default.
• W2147918084 modified "2023-09-26" @default.
• W2147918084 title "Recombination in bacteriophage lambda DNA half molecules" @default.
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• W2147918084 doi "https://doi.org/10.1016/s0022-2836(72)80004-4" @default.
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