Matches in SemOpenAlex for { <https://semopenalex.org/work/W2147942782> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2147942782 endingPage "1164" @default.
- W2147942782 startingPage "1156" @default.
- W2147942782 abstract "The skeletal muscle sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA1a) mediates muscle relaxation by pumping Ca(2+) from the cytosol to the ER/SR lumen. In efforts aimed at understanding the structural basis for the conformational changes accompanying the reaction cycle catalyzed by SERCA1a, we have studied the ATP-binding domain of SERCA1a in both nucleotide-bound and -free forms by NMR. Limited proteolysis analyses guided us to express a 28 kDa stably folded fragment containing the nucleotide-binding domain of SERCA1a spanning residues Thr357-Leu600. ATP binding activity was demonstrated for this fragment by a FITC competition assay. A nearly complete backbone resonance assignment of this 28 kDa ATP-binding fragment, in both the AMP-PNP-bound and -free forms, was obtained by means of heteronuclear multidimensional NMR techniques. NMR titration experiments with AMP-PNP revealed a confined nucleotide-binding site which coincides with a cytoplasmic pocket region identified in the crystal structure of apo-SERCA1a. These results are consistent with previous site-directed mutagenesis studies of SERCA1a." @default.
- W2147942782 created "2016-06-24" @default.
- W2147942782 creator A5016424930 @default.
- W2147942782 creator A5058821876 @default.
- W2147942782 creator A5068839871 @default.
- W2147942782 creator A5076485810 @default.
- W2147942782 creator A5079457415 @default.
- W2147942782 date "2002-01-01" @default.
- W2147942782 modified "2023-09-27" @default.
- W2147942782 title "Characterization of the ATP-Binding Domain of the Sarco(endo)plasmic Reticulum Ca<sup>2+</sup>-ATPase: Probing Nucleotide Binding by Multidimensional NMR" @default.
- W2147942782 cites W1554368439 @default.
- W2147942782 cites W1593573243 @default.
- W2147942782 cites W1595204022 @default.
- W2147942782 cites W196653855 @default.
- W2147942782 cites W1977869047 @default.
- W2147942782 cites W2009740509 @default.
- W2147942782 cites W2013578935 @default.
- W2147942782 cites W2031292508 @default.
- W2147942782 cites W2034507844 @default.
- W2147942782 cites W2050424514 @default.
- W2147942782 cites W2055520562 @default.
- W2147942782 cites W2067020293 @default.
- W2147942782 cites W2076952391 @default.
- W2147942782 cites W2087726424 @default.
- W2147942782 cites W2137153304 @default.
- W2147942782 cites W2148521301 @default.
- W2147942782 cites W2149338396 @default.
- W2147942782 cites W2169821755 @default.
- W2147942782 cites W2176061808 @default.
- W2147942782 cites W2330094517 @default.
- W2147942782 cites W2417942693 @default.
- W2147942782 doi "https://doi.org/10.1021/bi015703n" @default.
- W2147942782 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11802714" @default.
- W2147942782 hasPublicationYear "2002" @default.
- W2147942782 type Work @default.
- W2147942782 sameAs 2147942782 @default.
- W2147942782 citedByCount "34" @default.
- W2147942782 countsByYear W21479427822012 @default.
- W2147942782 countsByYear W21479427822013 @default.
- W2147942782 countsByYear W21479427822015 @default.
- W2147942782 countsByYear W21479427822016 @default.
- W2147942782 countsByYear W21479427822018 @default.
- W2147942782 countsByYear W21479427822019 @default.
- W2147942782 countsByYear W21479427822020 @default.
- W2147942782 countsByYear W21479427822022 @default.
- W2147942782 crossrefType "journal-article" @default.
- W2147942782 hasAuthorship W2147942782A5016424930 @default.
- W2147942782 hasAuthorship W2147942782A5058821876 @default.
- W2147942782 hasAuthorship W2147942782A5068839871 @default.
- W2147942782 hasAuthorship W2147942782A5076485810 @default.
- W2147942782 hasAuthorship W2147942782A5079457415 @default.
- W2147942782 hasConcept C104317684 @default.
- W2147942782 hasConcept C107824862 @default.
- W2147942782 hasConcept C181199279 @default.
- W2147942782 hasConcept C185592680 @default.
- W2147942782 hasConcept C23265538 @default.
- W2147942782 hasConcept C512185932 @default.
- W2147942782 hasConcept C55493867 @default.
- W2147942782 hasConcept C66974803 @default.
- W2147942782 hasConcept C71240020 @default.
- W2147942782 hasConcept C8010536 @default.
- W2147942782 hasConcept C92381491 @default.
- W2147942782 hasConceptScore W2147942782C104317684 @default.
- W2147942782 hasConceptScore W2147942782C107824862 @default.
- W2147942782 hasConceptScore W2147942782C181199279 @default.
- W2147942782 hasConceptScore W2147942782C185592680 @default.
- W2147942782 hasConceptScore W2147942782C23265538 @default.
- W2147942782 hasConceptScore W2147942782C512185932 @default.
- W2147942782 hasConceptScore W2147942782C55493867 @default.
- W2147942782 hasConceptScore W2147942782C66974803 @default.
- W2147942782 hasConceptScore W2147942782C71240020 @default.
- W2147942782 hasConceptScore W2147942782C8010536 @default.
- W2147942782 hasConceptScore W2147942782C92381491 @default.
- W2147942782 hasIssue "4" @default.
- W2147942782 hasLocation W21479427821 @default.
- W2147942782 hasLocation W21479427822 @default.
- W2147942782 hasOpenAccess W2147942782 @default.
- W2147942782 hasPrimaryLocation W21479427821 @default.
- W2147942782 hasRelatedWork W1595435363 @default.
- W2147942782 hasRelatedWork W1990459484 @default.
- W2147942782 hasRelatedWork W1997521944 @default.
- W2147942782 hasRelatedWork W2005925287 @default.
- W2147942782 hasRelatedWork W2041613088 @default.
- W2147942782 hasRelatedWork W2048261270 @default.
- W2147942782 hasRelatedWork W2057512434 @default.
- W2147942782 hasRelatedWork W2065570520 @default.
- W2147942782 hasRelatedWork W2417278432 @default.
- W2147942782 hasRelatedWork W37300835 @default.
- W2147942782 hasVolume "41" @default.
- W2147942782 isParatext "false" @default.
- W2147942782 isRetracted "false" @default.
- W2147942782 magId "2147942782" @default.
- W2147942782 workType "article" @default.