FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2148022182> ?p ?o ?g. }

• W2148022182 abstract "The methylotrophic yeast Pichia pastoris is a popular heterologous expression host for the recombinant production of a variety of prokaryotic and eukaryotic proteins. The rapid emergence of P. pastoris as a robust heterologous expression host was facilitated by the ease with which it can be manipulated and propagated, which is comparable to that of Escherichia coli and Saccharomyces cerevisiae. P. pastoris offers further advantages such as the tightly-regulated alcohol oxidase promoter that is particularly suitable for heterologous expression of foreign genes. While recombinant production of bacterial toxins and their derivatives is highly desirable, attempts at their heterologous expression using the traditional E. coli expression system can be problematic due to the formation of inclusion bodies that often severely limit the final yields of biologically active products. However, recent literature now suggests that P. pastoris may be an attractive alternative host for the heterologous production of bacterial toxins, such as those from the genera Bacillus, Clostridium, and Corynebacterium, as well as their more complex derivatives. Here, we review the recombinant production of bacterial toxins and their derivatives in P. pastoris with special emphasis on their potential clinical applications. Considering that de novo design and construction of synthetic toxin genes have often been necessary to achieve optimal heterologous expression in P. pastoris, we also present general guidelines to this end based on our experience with the P. pastoris expression of the Bacillus thuringiensis Cyt2Aa1 toxin." @default.
• W2148022182 created "2016-06-24" @default.
• W2148022182 creator A5028687753 @default.
• W2148022182 creator A5067566367 @default.
• W2148022182 date "2005-12-01" @default.
• W2148022182 modified "2023-10-16" @default.
• W2148022182 title "Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris" @default.
• W2148022182 cites W100624870 @default.
• W2148022182 cites W1550695177 @default.
• W2148022182 cites W1603411458 @default.
• W2148022182 cites W1878649451 @default.
• W2148022182 cites W1885175685 @default.
• W2148022182 cites W1938341712 @default.
• W2148022182 cites W1965020882 @default.
• W2148022182 cites W1969531899 @default.
• W2148022182 cites W1983181891 @default.
• W2148022182 cites W1987007767 @default.
• W2148022182 cites W1988078730 @default.
• W2148022182 cites W1991858546 @default.
• W2148022182 cites W1994114266 @default.
• W2148022182 cites W1994984684 @default.
• W2148022182 cites W1995726762 @default.
• W2148022182 cites W2003228141 @default.
• W2148022182 cites W2008765994 @default.
• W2148022182 cites W2012502190 @default.
• W2148022182 cites W2013602233 @default.
• W2148022182 cites W2015292449 @default.
• W2148022182 cites W2018293950 @default.
• W2148022182 cites W2039201478 @default.
• W2148022182 cites W2040836809 @default.
• W2148022182 cites W2041721711 @default.
• W2148022182 cites W2045321526 @default.
• W2148022182 cites W2055323189 @default.
• W2148022182 cites W2058426472 @default.
• W2148022182 cites W2060093926 @default.
• W2148022182 cites W2060797308 @default.
• W2148022182 cites W2062086371 @default.
• W2148022182 cites W2064577218 @default.
• W2148022182 cites W2068109582 @default.
• W2148022182 cites W2071081390 @default.
• W2148022182 cites W2082581989 @default.
• W2148022182 cites W2095668363 @default.
• W2148022182 cites W2100024090 @default.
• W2148022182 cites W2104621536 @default.
• W2148022182 cites W2107847732 @default.
• W2148022182 cites W2108786034 @default.
• W2148022182 cites W2109058814 @default.
• W2148022182 cites W2120357935 @default.
• W2148022182 cites W2126444066 @default.
• W2148022182 cites W2137517206 @default.
• W2148022182 cites W2144213129 @default.
• W2148022182 cites W2146690870 @default.
• W2148022182 cites W2148358473 @default.
• W2148022182 cites W2152368084 @default.
• W2148022182 cites W2163264253 @default.
• W2148022182 cites W2172115284 @default.
• W2148022182 cites W2469487942 @default.
• W2148022182 cites W3098021590 @default.
• W2148022182 cites W4213151022 @default.
• W2148022182 cites W4236495084 @default.
• W2148022182 cites W4237588029 @default.
• W2148022182 cites W4245939111 @default.
• W2148022182 cites W4247412353 @default.
• W2148022182 doi "https://doi.org/10.1186/1475-2859-4-33" @default.
• W2148022182 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1325036" @default.
• W2148022182 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16336647" @default.
• W2148022182 hasPublicationYear "2005" @default.
• W2148022182 type Work @default.
• W2148022182 sameAs 2148022182 @default.
• W2148022182 citedByCount "42" @default.
• W2148022182 countsByYear W21480221822012 @default.
• W2148022182 countsByYear W21480221822013 @default.
• W2148022182 countsByYear W21480221822014 @default.
• W2148022182 countsByYear W21480221822015 @default.
• W2148022182 countsByYear W21480221822016 @default.
• W2148022182 countsByYear W21480221822017 @default.
• W2148022182 countsByYear W21480221822018 @default.
• W2148022182 countsByYear W21480221822019 @default.
• W2148022182 countsByYear W21480221822021 @default.
• W2148022182 countsByYear W21480221822023 @default.
• W2148022182 crossrefType "journal-article" @default.
• W2148022182 hasAuthorship W2148022182A5028687753 @default.
• W2148022182 hasAuthorship W2148022182A5067566367 @default.
• W2148022182 hasBestOaLocation W21480221821 @default.
• W2148022182 hasConcept C104317684 @default.
• W2148022182 hasConcept C108305142 @default.
• W2148022182 hasConcept C2777576037 @default.
• W2148022182 hasConcept C2779222958 @default.
• W2148022182 hasConcept C2779703480 @default.
• W2148022182 hasConcept C2780115692 @default.
• W2148022182 hasConcept C2781372317 @default.
• W2148022182 hasConcept C40767141 @default.
• W2148022182 hasConcept C547475151 @default.
• W2148022182 hasConcept C55493867 @default.
• W2148022182 hasConcept C86803240 @default.
• W2148022182 hasConcept C89423630 @default.
• W2148022182 hasConceptScore W2148022182C104317684 @default.
• W2148022182 hasConceptScore W2148022182C108305142 @default.
• W2148022182 hasConceptScore W2148022182C2777576037 @default.
• W2148022182 hasConceptScore W2148022182C2779222958 @default.

• next