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- W2148286918 abstract "ABSTRACT Two protocols are presented that allow the selection of mutations mapping to micronuclear chromosome 5 in Tetrahymena thermophilia. One protocol involves crossing mutagenized diploid cells directly to a strain nullisomic for chromosome 5 and screening the monosomic progeny for a mutant phenotype. The second protocol first takes the mutagenized diploid cells through round I of genomic exclusion to create useful and reusable mutant heterokaryons, which are then assayed for the presence of mutations on chromosome 5 by crossing to the nullisomic 5 strain. Of 14 putative chromosome 5 mutations obtained by these two methods, seven are shown by genetic analysis to be recessive mutations on chromosome 5; one mapped elsewhere in the genome; six were infertile or failed to yield progeny in some of the diagnostic crosses and, thus, their genetic nature could not be determined with certainty." @default.
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- W2148286918 date "1984-03-01" @default.
- W2148286918 modified "2023-10-17" @default.
- W2148286918 title "CHROMOSOME-DESIGNATED MUTATION SELECTION IN TETRAHYMENA THERMOPHILA" @default.
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- W2148286918 doi "https://doi.org/10.1093/genetics/106.3.387" @default.
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