FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2148291723> ?p ?o ?g. }

• W2148291723 endingPage "95" @default.
• W2148291723 startingPage "86" @default.
• W2148291723 abstract "CD20 has been proposed as a novel suicide gene system for the treatment of graft-versus-host disease (GVHD), a fatal complication of allogeneic bone marrow transplantation: indeed expression of the human non-immunogenic exogenous CD20 protein allows positive immunoselection of transduced cells as well as their killing in vitro with rituximab. Lentiviral vectors are promising tools in the field of gene therapy. We therefore searched for a lentivector giving good efficiency of transduction of human T lymphocytes activated by the sole addition of interleukin (IL)-2 and high expression levels of the CD20 transgene.The T cell line CEM and peripheral T lymphocytes activated by phytohemagglutinin (PHA) and/or IL-2 were transduced with two different vectors carrying the CD20 transgene driven by either the phosphoglycerate kinase (PGK) or elongation factor 1alpha (EF1alpha) promoter, and using different multiplicities of infection (MOIs).Both the PGK- and EF1alpha-CD20 vectors allowed efficient transduction of the CEM cell line and PHA-activated T cells, reaching 99 and 90% in the different targets, respectively. However EF1alpha-CD20 led to much higher expression levels of the transgene (mean fluorescence intensity 588-618 compared to 53 for PGK-CD20). Furthermore lymphocytes activated with IL-2 alone could be efficiently transduced with EF1alpha-CD20, reaching 10-25% positivity for CD20 (mean fluorescence intensity 409-424), allowing adequate immunoselection and strong complement-mediated lysis.EF1alpha-CD20 may represent a good candidate vector for gene therapy with the CD20 suicide system in the setting of allogeneic bone marrow transplants." @default.
• W2148291723 created "2016-06-24" @default.
• W2148291723 creator A5002997724 @default.
• W2148291723 creator A5018208681 @default.
• W2148291723 creator A5036565811 @default.
• W2148291723 creator A5051058036 @default.
• W2148291723 date "2004-01-01" @default.
• W2148291723 modified "2023-10-18" @default.
• W2148291723 title "Elongation factor 1 (EF1alpha) promoter in a lentiviral backbone improves expression of the CD20 suicide gene in primary T lymphocytes allowing efficient rituximab-mediated lysis." @default.
• W2148291723 cites W1566969252 @default.
• W2148291723 cites W1647478418 @default.
• W2148291723 cites W1707184873 @default.
• W2148291723 cites W18341126 @default.
• W2148291723 cites W1915689252 @default.
• W2148291723 cites W1966279605 @default.
• W2148291723 cites W1967338869 @default.
• W2148291723 cites W1971384581 @default.
• W2148291723 cites W1975678319 @default.
• W2148291723 cites W1977308392 @default.
• W2148291723 cites W197816702 @default.
• W2148291723 cites W1978657243 @default.
• W2148291723 cites W1981480494 @default.
• W2148291723 cites W1982206175 @default.
• W2148291723 cites W1983339176 @default.
• W2148291723 cites W1984923085 @default.
• W2148291723 cites W1986115228 @default.
• W2148291723 cites W1987746867 @default.
• W2148291723 cites W1988121435 @default.
• W2148291723 cites W1988614032 @default.
• W2148291723 cites W1994279077 @default.
• W2148291723 cites W1998609613 @default.
• W2148291723 cites W2002857052 @default.
• W2148291723 cites W2004373851 @default.
• W2148291723 cites W2009586982 @default.
• W2148291723 cites W2009761723 @default.
• W2148291723 cites W2024875691 @default.
• W2148291723 cites W2025859796 @default.
• W2148291723 cites W2030504351 @default.
• W2148291723 cites W2037105618 @default.
• W2148291723 cites W2037669641 @default.
• W2148291723 cites W2038405360 @default.
• W2148291723 cites W2041438140 @default.
• W2148291723 cites W2047028501 @default.
• W2148291723 cites W2055393026 @default.
• W2148291723 cites W2059601682 @default.
• W2148291723 cites W2069524809 @default.
• W2148291723 cites W2075288748 @default.
• W2148291723 cites W2082635925 @default.
• W2148291723 cites W2089049593 @default.
• W2148291723 cites W2090010312 @default.
• W2148291723 cites W2091432200 @default.
• W2148291723 cites W2091610940 @default.
• W2148291723 cites W2091987058 @default.
• W2148291723 cites W2097606337 @default.
• W2148291723 cites W2112041130 @default.
• W2148291723 cites W2117486036 @default.
• W2148291723 cites W2147068163 @default.
• W2148291723 cites W2159861616 @default.
• W2148291723 cites W2160587796 @default.
• W2148291723 cites W2167523976 @default.
• W2148291723 cites W2168190597 @default.
• W2148291723 cites W2171653261 @default.
• W2148291723 cites W2175611237 @default.
• W2148291723 cites W2180651989 @default.
• W2148291723 cites W2180938329 @default.
• W2148291723 cites W2323613689 @default.
• W2148291723 cites W2327526254 @default.
• W2148291723 cites W2328574330 @default.
• W2148291723 cites W2330203959 @default.
• W2148291723 cites W2330290895 @default.
• W2148291723 cites W2335074884 @default.
• W2148291723 cites W2418889500 @default.
• W2148291723 cites W28588605 @default.
• W2148291723 cites W33733012 @default.
• W2148291723 cites W60759546 @default.
• W2148291723 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14754610" @default.
• W2148291723 hasPublicationYear "2004" @default.
• W2148291723 type Work @default.
• W2148291723 sameAs 2148291723 @default.
• W2148291723 citedByCount "4" @default.
• W2148291723 countsByYear W21482917232018 @default.
• W2148291723 countsByYear W21482917232019 @default.
• W2148291723 crossrefType "journal-article" @default.
• W2148291723 hasAuthorship W2148291723A5002997724 @default.
• W2148291723 hasAuthorship W2148291723A5018208681 @default.
• W2148291723 hasAuthorship W2148291723A5036565811 @default.
• W2148291723 hasAuthorship W2148291723A5051058036 @default.
• W2148291723 hasConcept C102230213 @default.
• W2148291723 hasConcept C104317684 @default.
• W2148291723 hasConcept C111599444 @default.
• W2148291723 hasConcept C15152581 @default.
• W2148291723 hasConcept C153911025 @default.
• W2148291723 hasConcept C159047783 @default.
• W2148291723 hasConcept C159654299 @default.
• W2148291723 hasConcept C18031839 @default.
• W2148291723 hasConcept C203014093 @default.
• W2148291723 hasConcept C32470452 @default.
• W2148291723 hasConcept C34981463 @default.

• next