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- W2148402717 abstract "Long interspersed element-1 (LINE-1 or L1) retrotransposons account for nearly 17% of human genomic DNA and represent a major evolutionary force that has reshaped the structure and function of the human genome. However, questions remain concerning both the frequency and the developmental timing of L1 retrotransposition in vivo and whether the mobility of these retroelements commonly results in insertional and post-insertional mechanisms of genomic injury. Cells exhibiting high rates of L1 retrotransposition might be especially at risk for such injury. We assessed L1 mRNA expression and L1 retrotransposition in two biologically relevant cell types, human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), as well as in control parental human dermal fibroblasts (HDFs). Full-length L1 mRNA and the L1 open reading frame 1-encoded protein (ORF1p) were readily detected in hESCs and iPSCs, but not in HDFs. Sequencing analysis proved the expression of human-specific L1 element mRNAs in iPSCs. Bisulfite sequencing revealed that the increased L1 expression observed in iPSCs correlates with an overall decrease in CpG methylation in the L1 promoter region. Finally, retrotransposition of an engineered human L1 element was ∼10-fold more efficient in iPSCs than in parental HDFs. These findings indicate that somatic cell reprogramming is associated with marked increases in L1 expression and perhaps increases in endogenous L1 retrotransposition, which could potentially impact the genomic integrity of the resultant iPSCs." @default.
- W2148402717 created "2016-06-24" @default.
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- W2148402717 date "2011-10-11" @default.
- W2148402717 modified "2023-10-15" @default.
- W2148402717 title "Reprogramming somatic cells into iPS cells activates LINE-1 retroelement mobility" @default.
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- W2148402717 cites W1944771638 @default.
- W2148402717 cites W1978596306 @default.
- W2148402717 cites W1985575613 @default.
- W2148402717 cites W1987332588 @default.
- W2148402717 cites W1998154426 @default.
- W2148402717 cites W1999355295 @default.
- W2148402717 cites W1999759851 @default.
- W2148402717 cites W1999884745 @default.
- W2148402717 cites W2002118607 @default.
- W2148402717 cites W2002190096 @default.
- W2148402717 cites W2005764402 @default.
- W2148402717 cites W2007589425 @default.
- W2148402717 cites W2012537642 @default.
- W2148402717 cites W2014439672 @default.
- W2148402717 cites W2015415562 @default.
- W2148402717 cites W2019229253 @default.
- W2148402717 cites W2019469673 @default.
- W2148402717 cites W2023865653 @default.
- W2148402717 cites W2035120673 @default.
- W2148402717 cites W2036300762 @default.
- W2148402717 cites W2036994672 @default.
- W2148402717 cites W2037509327 @default.
- W2148402717 cites W2046415390 @default.
- W2148402717 cites W2048560318 @default.
- W2148402717 cites W2051541780 @default.
- W2148402717 cites W2054066197 @default.
- W2148402717 cites W2054143743 @default.
- W2148402717 cites W2055705164 @default.
- W2148402717 cites W2059378220 @default.
- W2148402717 cites W2064629259 @default.
- W2148402717 cites W2070807615 @default.
- W2148402717 cites W2075224242 @default.
- W2148402717 cites W2077053535 @default.
- W2148402717 cites W2085758832 @default.
- W2148402717 cites W2086663462 @default.
- W2148402717 cites W2086739233 @default.
- W2148402717 cites W2086943348 @default.
- W2148402717 cites W2089471275 @default.
- W2148402717 cites W2091279754 @default.
- W2148402717 cites W2093606206 @default.
- W2148402717 cites W2096910509 @default.
- W2148402717 cites W2097851896 @default.
- W2148402717 cites W2098666676 @default.
- W2148402717 cites W2099468911 @default.
- W2148402717 cites W2102773855 @default.
- W2148402717 cites W2104430230 @default.
- W2148402717 cites W2113973815 @default.
- W2148402717 cites W2118734051 @default.
- W2148402717 cites W2119742125 @default.
- W2148402717 cites W2120034162 @default.
- W2148402717 cites W2123883764 @default.
- W2148402717 cites W2126432370 @default.
- W2148402717 cites W2131212168 @default.
- W2148402717 cites W2136460560 @default.
- W2148402717 cites W2138977668 @default.
- W2148402717 cites W2142046859 @default.
- W2148402717 cites W2145932909 @default.
- W2148402717 cites W2146892790 @default.
- W2148402717 cites W2150649207 @default.
- W2148402717 cites W2153593912 @default.
- W2148402717 cites W2155264063 @default.
- W2148402717 cites W2157890550 @default.
- W2148402717 cites W2160548451 @default.
- W2148402717 cites W2162146849 @default.
- W2148402717 cites W2165618724 @default.
- W2148402717 cites W2168676279 @default.
- W2148402717 cites W2168909179 @default.
- W2148402717 cites W2171537206 @default.
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- W2148402717 doi "https://doi.org/10.1093/hmg/ddr455" @default.
- W2148402717 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3235014" @default.
- W2148402717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21989055" @default.
- W2148402717 hasPublicationYear "2011" @default.
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