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- W2148510010 abstract "Mice were injected with a range of bromodeoxyuridine (BrdU) concentrations from 0.01 mg to 10 mg, and their jaws were fixed in buffered formalin or modified Carnoy. After EDTA or formic acid decalcification, a range of DNA denaturation schedules was assessed and immunohistological detection of BrdU-containing nuclei was performed using the Sera Lab anti-BrdU antibody MAS 250b. For Carnoy-fixed tissue, denaturation in 1 N HCl for 8 min at 60 degrees C was capable of adequately detecting an injected dose of 0.05 mg but not a dose of 0.01 mg BrdU, whereas pepsin/HCl treatment gave only weak staining after injection of 1 mg BrdU. In comparison, formalin fixation required pre-treatment with 0.2-0.4% pepsin/HCl at 37 degrees C for comparable staining intensity, but could still not adequately detect a dose of 0.1 mg BrdU. There was little detectable difference in staining between EDTA- and formic acid-decalcified tissues after injection of 10 mg BrdU." @default.
- W2148510010 created "2016-06-24" @default.
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- W2148510010 date "1990-04-01" @default.
- W2148510010 modified "2023-10-15" @default.
- W2148510010 title "Immunohistological optimization of detection of bromodeoxyuridine-labeled cells in decalcified tissue." @default.
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- W2148510010 doi "https://doi.org/10.1177/38.4.2108204" @default.
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