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• W2148519189 endingPage "1377" @default.
• W2148519189 startingPage "1369" @default.
• W2148519189 abstract "Polymerase chain reactions (PCR), thermally activated chemical reactions which are widely used for nucleic acid amplification, have recently received much attention in microelectromechanical systems and micro total analysis systems because a wide variety of DNA/RNA molecules can be enriched by PCR for further analyses. In the present work, a droplet-based micro oscillating-flow PCR chip was designed and fabricated by the silicon microfabrication technique. Three different temperature zones, which were stable at denaturation, extension and annealing temperatures and isolated from each other by a thin-wall linkage, were integrated with a single, simple and straight microchannel to form the chip's basic functional structure. The PCR mixture was injected into the chip as a single droplet and flowed through the three temperature zones in the main microchannel in an oscillating manner to achieve the temperature maintenance and transitions. The chip's thermal performance was theoretically analyzed and numerically simulated. The results indicated that the time needed for the temperature of the droplet to change to the target value is less than 1 s, and the root mean square error of temperature is less than 0.2 °C. A droplet of 1 µl PCR mixture with standard HPV (Human Papilloma Virus)-DNA sample inside was amplified by the present chip and the results were analyzed by slab gel electrophoresis with separation of DNA markers in parallel. The electrophoresis results demonstrated that the micro oscillating-flow PCR chip successfully amplified the HPV-DNA, with a processing time of about 15 min which is significantly reduced compared to that for the conventional PCR instrument." @default.
• W2148519189 created "2016-06-24" @default.
• W2148519189 creator A5049801163 @default.
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• W2148519189 date "2005-05-25" @default.
• W2148519189 modified "2023-09-28" @default.
• W2148519189 title "Droplet-based micro oscillating-flow PCR chip" @default.
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• W2148519189 doi "https://doi.org/10.1088/0960-1317/15/8/001" @default.
• W2148519189 hasPublicationYear "2005" @default.
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