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• W2148583927 endingPage "3241" @default.
• W2148583927 startingPage "3237" @default.
• W2148583927 abstract "We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The feasibility and the increase in detection sensitivity of microscopic imaging of in situ hybridized, fluoresceinated DNA with an integrating solid state camera for rapid cosmid mapping is illustrated. Combination of fluoresceinated DNA with biotinated and digoxigeninated DNAs allowed easy performance of triple fluorescence in situ hybridization. The potential of these techniques for DNA mapping, cytogenetics and biological dosimetry is briefly discussed." @default.
• W2148583927 created "2016-06-24" @default.
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• W2148583927 date "1991-01-01" @default.
• W2148583927 modified "2023-10-14" @default.
• W2148583927 title "<i>In situ</i>hybridisation with fluoresceinated DNA" @default.
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• W2148583927 doi "https://doi.org/10.1093/nar/19.12.3237" @default.
• W2148583927 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/328316" @default.
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