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• W2148689258 abstract "The gene xylB(ADP1) from Acinetobacter baylyi ADP1 (gene annotation number ACIAD1578), coding for a putative aryl alcohol dehydrogenase, was heterologously expressed in Escherichia coli BL21(DE3). The respective aryl alcohol dehydrogenase was purified by fast protein liquid chromatography to apparent electrophoretic homogeneity. The predicted molecular weight of 39,500 per subunit was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. According to the native M(w) as determined by gel filtration, the enzyme forms dimers and therefore seems to be XylB related. The enzyme showed the highest activity at 40°C. For both the reduction and the oxidation reactions, the pH for optimum activity was 6.5. The enzyme was NADH dependent and able to reduce medium- to long-chain n-alkylaldehydes, methyl-branched aldehydes, and aromatic aldehydes, with benzaldehyde yielding the highest activity. The oxidation reaction with the corresponding alcohols showed only 2.2% of the reduction activity, with coniferyl alcohol yielding the highest activity. Maximum activities for the reduction and the oxidation reaction were 104.5 and 2.3 U mg(-1) of protein, respectively. The enzyme activity was affected by low concentrations of Ag(+) and Hg(2+) and high concentrations of Cu(2+), Zn(2+), and Fe(2+). The gene xylB(ADP1) seems to be expressed constitutively and an involvement in coniferyl alcohol degradation is suggested. However, the enzyme is most probably not involved in the degradation of benzyl alcohol, anisalcohol, salicyl alcohol, vanillyl alcohol, cinnamyl alcohol, or aliphatic and isoprenoid alcohols." @default.
• W2148689258 created "2016-06-24" @default.
• W2148689258 creator A5060240132 @default.
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• W2148689258 date "2012-12-15" @default.
• W2148689258 modified "2023-10-02" @default.
• W2148689258 title "Purification and Characterization of an NAD ⁺ -Dependent XylB-Like Aryl Alcohol Dehydrogenase Identified in Acinetobacter baylyi ADP1" @default.
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• W2148689258 doi "https://doi.org/10.1128/aem.02224-12" @default.
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