FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2148764180> ?p ?o ?g. }

• W2148764180 endingPage "1527" @default.
• W2148764180 startingPage "1520" @default.
• W2148764180 abstract "Nitric oxide (NO), synthesized by the inducible nitric oxide synthase (iNOS), is known to inhibit metabolic oxygen consumption because of interference with mitochondrial respiratory activity. This study examined whether activation of iNOS (a) directly in tumor cells or (b) in bystander macrophages may improve radioresponse through sparing of oxygen.EMT-6 tumor cells and RAW 264.7 macrophages were exposed to bacterial lipopolysaccharide plus interferon-gamma, and examined for iNOS expression by reverse transcription polymerase chain reaction, Western blotting and enzymatic activity. Tumor cells alone, or combined with macrophages were subjected to metabolic hypoxia and analyzed for radiosensitivity by clonogenic assay, and for oxygen consumption by electron paramagnetic resonance and a Clark-type electrode.Both tumor cells and macrophages displayed a coherent picture of iNOS induction at transcriptional/translational levels and NO/nitrite production, whereas macrophages showed also co-induction of the inducible heme oxygenase-1, which is associated with carbon monoxide (CO) and bilirubin production. Activation of iNOS in tumor cells resulted in a profound oxygen sparing and a 2.3-fold radiosensitization. Bystander NO-producing, but not CO-producing, macrophages were able to block oxygen consumption by 1.9-fold and to radiosensitize tumor cells by 2.2-fold. Both effects could be neutralized by aminoguanidine, a metabolic iNOS inhibitor. An improved radioresponse was clearly observed at macrophages to tumor cells ratios ranging between 1:16 to 1:1.Our study is the first, as far as we are aware, to provide evidence that iNOS may induce radiosensitization through oxygen sparing, and illuminates NO-producing macrophages as a novel determinant of tumor cell radioresponse within the hypoxic tumor microenvironment." @default.
• W2148764180 created "2016-06-24" @default.
• W2148764180 creator A5000316108 @default.
• W2148764180 creator A5002756893 @default.
• W2148764180 creator A5003934435 @default.
• W2148764180 creator A5010279808 @default.
• W2148764180 creator A5023765183 @default.
• W2148764180 creator A5024554584 @default.
• W2148764180 creator A5040579751 @default.
• W2148764180 creator A5040879275 @default.
• W2148764180 creator A5072112944 @default.
• W2148764180 creator A5074877036 @default.
• W2148764180 creator A5080670960 @default.
• W2148764180 creator A5089177738 @default.
• W2148764180 date "2010-04-01" @default.
• W2148764180 modified "2023-09-27" @default.
• W2148764180 title "Activated Macrophages as a Novel Determinant of Tumor Cell Radioresponse: The Role of Nitric Oxide–Mediated Inhibition of Cellular Respiration and Oxygen Sparing" @default.
• W2148764180 cites W1967573722 @default.
• W2148764180 cites W1969296030 @default.
• W2148764180 cites W1969592847 @default.
• W2148764180 cites W1984803858 @default.
• W2148764180 cites W1990665368 @default.
• W2148764180 cites W1994273386 @default.
• W2148764180 cites W1996830477 @default.
• W2148764180 cites W2009455086 @default.
• W2148764180 cites W2037020879 @default.
• W2148764180 cites W2059799711 @default.
• W2148764180 cites W2074711725 @default.
• W2148764180 cites W2089774193 @default.
• W2148764180 cites W2131464730 @default.
• W2148764180 cites W2135783367 @default.
• W2148764180 cites W2142256588 @default.
• W2148764180 cites W2153330586 @default.
• W2148764180 cites W2160267495 @default.
• W2148764180 cites W2163715194 @default.
• W2148764180 cites W2166597180 @default.
• W2148764180 cites W2170273562 @default.
• W2148764180 doi "https://doi.org/10.1016/j.ijrobp.2009.10.047" @default.
• W2148764180 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20338478" @default.
• W2148764180 hasPublicationYear "2010" @default.
• W2148764180 type Work @default.
• W2148764180 sameAs 2148764180 @default.
• W2148764180 citedByCount "29" @default.
• W2148764180 countsByYear W21487641802012 @default.
• W2148764180 countsByYear W21487641802013 @default.
• W2148764180 countsByYear W21487641802014 @default.
• W2148764180 countsByYear W21487641802015 @default.
• W2148764180 countsByYear W21487641802016 @default.
• W2148764180 countsByYear W21487641802017 @default.
• W2148764180 countsByYear W21487641802018 @default.
• W2148764180 countsByYear W21487641802019 @default.
• W2148764180 countsByYear W21487641802020 @default.
• W2148764180 countsByYear W21487641802022 @default.
• W2148764180 countsByYear W21487641802023 @default.
• W2148764180 crossrefType "journal-article" @default.
• W2148764180 hasAuthorship W2148764180A5000316108 @default.
• W2148764180 hasAuthorship W2148764180A5002756893 @default.
• W2148764180 hasAuthorship W2148764180A5003934435 @default.
• W2148764180 hasAuthorship W2148764180A5010279808 @default.
• W2148764180 hasAuthorship W2148764180A5023765183 @default.
• W2148764180 hasAuthorship W2148764180A5024554584 @default.
• W2148764180 hasAuthorship W2148764180A5040579751 @default.
• W2148764180 hasAuthorship W2148764180A5040879275 @default.
• W2148764180 hasAuthorship W2148764180A5072112944 @default.
• W2148764180 hasAuthorship W2148764180A5074877036 @default.
• W2148764180 hasAuthorship W2148764180A5080670960 @default.
• W2148764180 hasAuthorship W2148764180A5089177738 @default.
• W2148764180 hasConcept C126322002 @default.
• W2148764180 hasConcept C153911025 @default.
• W2148764180 hasConcept C178790620 @default.
• W2148764180 hasConcept C181199279 @default.
• W2148764180 hasConcept C185592680 @default.
• W2148764180 hasConcept C19106626 @default.
• W2148764180 hasConcept C203014093 @default.
• W2148764180 hasConcept C206684579 @default.
• W2148764180 hasConcept C2776217839 @default.
• W2148764180 hasConcept C2777622882 @default.
• W2148764180 hasConcept C2778754761 @default.
• W2148764180 hasConcept C2779219270 @default.
• W2148764180 hasConcept C502942594 @default.
• W2148764180 hasConcept C509974204 @default.
• W2148764180 hasConcept C519581460 @default.
• W2148764180 hasConcept C540031477 @default.
• W2148764180 hasConcept C55493867 @default.
• W2148764180 hasConcept C71924100 @default.
• W2148764180 hasConcept C86803240 @default.
• W2148764180 hasConceptScore W2148764180C126322002 @default.
• W2148764180 hasConceptScore W2148764180C153911025 @default.
• W2148764180 hasConceptScore W2148764180C178790620 @default.
• W2148764180 hasConceptScore W2148764180C181199279 @default.
• W2148764180 hasConceptScore W2148764180C185592680 @default.
• W2148764180 hasConceptScore W2148764180C19106626 @default.
• W2148764180 hasConceptScore W2148764180C203014093 @default.
• W2148764180 hasConceptScore W2148764180C206684579 @default.
• W2148764180 hasConceptScore W2148764180C2776217839 @default.
• W2148764180 hasConceptScore W2148764180C2777622882 @default.
• W2148764180 hasConceptScore W2148764180C2778754761 @default.
• W2148764180 hasConceptScore W2148764180C2779219270 @default.

• next