FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2148966913> ?p ?o ?g. }

• W2148966913 endingPage "1748" @default.
• W2148966913 startingPage "1741" @default.
• W2148966913 abstract "We explored matrix-assisted laser desorption/ionization (MALDI) tandem time-of-flight (TOF/TOF) mass spectrometry for the analysis of N-glycosylated peptides, using horseradish peroxidase (HRP) as a test case. Two different types of cleavage were observed in the TOF/TOF fragmentation spectra: Firstly, cleavages of peptide bonds yielded fragments with the attached N-glycans staying intact, thus revealing information on peptide sequence and glycan attachment site. Secondly, fragmentation of the glycan moiety was characterized by cleavage of glycosidic bonds as well as a (0,2)X-ring fragmentation of the innermost N-acetylglucosamine of the chitobiose core. Loss of the complete N-glycan moiety occurred by cleavage of both the N-glycosidic bond and the side-chain amide group of the N-glycosylated asparagine, yielding a characteristic peak doublet with a mass difference of 17 Da, which revealed the individual masses of the N-glycan and the peptide moiety. Analysis of a HRP tryptic digest at the sub-picomole level allowed the characterization of various N-glycosylated peptides including those with internal disulfide linkages, a glycopeptide linked via a disulfide bond to another peptide, and a 5 kDa glycopeptide carrying two N-glycans. The potential of our approach was illustrated by the detection of the following novel features of HRP glycosylation: (i) The conjugation of a xylosylated trimannosyl N-glycan without core-fucosylation to site Asn316, showing for the first time unambiguously the occupation of this site; and (ii) A disaccharide N-acetylhexosamine1deoxyhexose1 attached to N-glycosylation sites Asn285 and Asn298, which might represent a Fuc(alpha1-3)GlcNAc- moiety arising from the processing of N-glycans by a horse-radish endoglycosidase during biosynthesis of HRP." @default.
• W2148966913 created "2016-06-24" @default.
• W2148966913 creator A5039947825 @default.
• W2148966913 creator A5055418983 @default.
• W2148966913 creator A5065397150 @default.
• W2148966913 date "2004-07-02" @default.
• W2148966913 modified "2023-10-09" @default.
• W2148966913 title "Glycopeptide analysis by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry reveals novel features of horseradish peroxidase glycosylation" @default.
• W2148966913 cites W1571588501 @default.
• W2148966913 cites W1964850706 @default.
• W2148966913 cites W1976165460 @default.
• W2148966913 cites W1977303047 @default.
• W2148966913 cites W1978078729 @default.
• W2148966913 cites W1980017824 @default.
• W2148966913 cites W1989465860 @default.
• W2148966913 cites W1992930552 @default.
• W2148966913 cites W1999159080 @default.
• W2148966913 cites W2004843282 @default.
• W2148966913 cites W2006342093 @default.
• W2148966913 cites W2006822851 @default.
• W2148966913 cites W2010712255 @default.
• W2148966913 cites W2011609623 @default.
• W2148966913 cites W2014194130 @default.
• W2148966913 cites W2015884002 @default.
• W2148966913 cites W2032145551 @default.
• W2148966913 cites W2033087747 @default.
• W2148966913 cites W2034178003 @default.
• W2148966913 cites W2044086315 @default.
• W2148966913 cites W2045817846 @default.
• W2148966913 cites W2046674035 @default.
• W2148966913 cites W2047566749 @default.
• W2148966913 cites W2051734899 @default.
• W2148966913 cites W2052099654 @default.
• W2148966913 cites W2055944866 @default.
• W2148966913 cites W2064140463 @default.
• W2148966913 cites W2065113620 @default.
• W2148966913 cites W2067199156 @default.
• W2148966913 cites W2069145584 @default.
• W2148966913 cites W2072131757 @default.
• W2148966913 cites W2074947572 @default.
• W2148966913 cites W2092560671 @default.
• W2148966913 cites W2142968365 @default.
• W2148966913 cites W2150197625 @default.
• W2148966913 cites W2154591143 @default.
• W2148966913 cites W2401534377 @default.
• W2148966913 cites W2953019130 @default.
• W2148966913 doi "https://doi.org/10.1002/rcm.1546" @default.
• W2148966913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15282773" @default.
• W2148966913 hasPublicationYear "2004" @default.
• W2148966913 type Work @default.
• W2148966913 sameAs 2148966913 @default.
• W2148966913 citedByCount "90" @default.
• W2148966913 countsByYear W21489669132012 @default.
• W2148966913 countsByYear W21489669132013 @default.
• W2148966913 countsByYear W21489669132014 @default.
• W2148966913 countsByYear W21489669132015 @default.
• W2148966913 countsByYear W21489669132016 @default.
• W2148966913 countsByYear W21489669132017 @default.
• W2148966913 countsByYear W21489669132018 @default.
• W2148966913 countsByYear W21489669132019 @default.
• W2148966913 countsByYear W21489669132020 @default.
• W2148966913 countsByYear W21489669132021 @default.
• W2148966913 countsByYear W21489669132022 @default.
• W2148966913 countsByYear W21489669132023 @default.
• W2148966913 crossrefType "journal-article" @default.
• W2148966913 hasAuthorship W2148966913A5039947825 @default.
• W2148966913 hasAuthorship W2148966913A5055418983 @default.
• W2148966913 hasAuthorship W2148966913A5065397150 @default.
• W2148966913 hasConcept C108625454 @default.
• W2148966913 hasConcept C137610392 @default.
• W2148966913 hasConcept C150394285 @default.
• W2148966913 hasConcept C150487720 @default.
• W2148966913 hasConcept C162356407 @default.
• W2148966913 hasConcept C162711632 @default.
• W2148966913 hasConcept C178790620 @default.
• W2148966913 hasConcept C181199279 @default.
• W2148966913 hasConcept C185592680 @default.
• W2148966913 hasConcept C206212055 @default.
• W2148966913 hasConcept C2777313579 @default.
• W2148966913 hasConcept C2777676563 @default.
• W2148966913 hasConcept C2779212266 @default.
• W2148966913 hasConcept C2779281246 @default.
• W2148966913 hasConcept C31684184 @default.
• W2148966913 hasConcept C31827203 @default.
• W2148966913 hasConcept C43617362 @default.
• W2148966913 hasConcept C501593827 @default.
• W2148966913 hasConcept C55493867 @default.
• W2148966913 hasConcept C71240020 @default.
• W2148966913 hasConcept C75280812 @default.
• W2148966913 hasConceptScore W2148966913C108625454 @default.
• W2148966913 hasConceptScore W2148966913C137610392 @default.
• W2148966913 hasConceptScore W2148966913C150394285 @default.
• W2148966913 hasConceptScore W2148966913C150487720 @default.
• W2148966913 hasConceptScore W2148966913C162356407 @default.
• W2148966913 hasConceptScore W2148966913C162711632 @default.
• W2148966913 hasConceptScore W2148966913C178790620 @default.
• W2148966913 hasConceptScore W2148966913C181199279 @default.
• W2148966913 hasConceptScore W2148966913C185592680 @default.

• next