FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2148974089> ?p ?o ?g. }

• W2148974089 endingPage "407" @default.
• W2148974089 startingPage "407" @default.
• W2148974089 abstract "MicroRNAs (miRNAs) have critical functions in various biological processes. MiRNA profiling is an important tool for the identification of differentially expressed miRNAs in normal cellular and disease processes. A technical challenge remains for high-throughput miRNA expression analysis as the number of miRNAs continues to increase with in silico prediction and experimental verification. Our study critically evaluated the performance of a novel miRNA expression profiling approach, quantitative RT-PCR array (qPCR-array), compared to miRNA detection with oligonucleotide microchip (microarray). High reproducibility with qPCR-array was demonstrated by comparing replicate results from the same RNA sample. Pre-amplification of the miRNA cDNA improved sensitivity of the qPCR-array and increased the number of detectable miRNAs. Furthermore, the relative expression levels of miRNAs were maintained after pre-amplification. When the performance of qPCR-array and microarrays were compared using different aliquots of the same RNA, a low correlation between the two methods (r = -0.443) indicated considerable variability between the two assay platforms. Higher variation between replicates was observed in miRNAs with low expression in both assays. Finally, a higher false positive rate of differential miRNA expression was observed using the microarray compared to the qPCR-array. Our studies demonstrated high reproducibility of TaqMan qPCR-array. Comparison between different reverse transcription reactions and qPCR-arrays performed on different days indicated that reverse transcription reactions did not introduce significant variation in the results. The use of cDNA pre-amplification increased the sensitivity of miRNA detection. Although there was variability associated with pre-amplification in low abundance miRNAs, the latter did not involve any systemic bias in the estimation of miRNA expression. Comparison between microarray and qPCR-array indicated superior sensitivity and specificity of qPCR-array." @default.
• W2148974089 created "2016-06-24" @default.
• W2148974089 creator A5014974894 @default.
• W2148974089 creator A5024582072 @default.
• W2148974089 creator A5058832369 @default.
• W2148974089 creator A5066940107 @default.
• W2148974089 date "2009-01-01" @default.
• W2148974089 modified "2023-10-10" @default.
• W2148974089 title "Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis" @default.
• W2148974089 cites W144423133 @default.
• W2148974089 cites W1484414601 @default.
• W2148974089 cites W1521385428 @default.
• W2148974089 cites W180484006 @default.
• W2148974089 cites W1965306643 @default.
• W2148974089 cites W1970847599 @default.
• W2148974089 cites W1997261348 @default.
• W2148974089 cites W2014946489 @default.
• W2148974089 cites W2017082263 @default.
• W2148974089 cites W2040008202 @default.
• W2148974089 cites W2042004955 @default.
• W2148974089 cites W2060905573 @default.
• W2148974089 cites W2064214040 @default.
• W2148974089 cites W2070571588 @default.
• W2148974089 cites W2079597727 @default.
• W2148974089 cites W2080327351 @default.
• W2148974089 cites W2091389016 @default.
• W2148974089 cites W2108244474 @default.
• W2148974089 cites W2108963354 @default.
• W2148974089 cites W2111655556 @default.
• W2148974089 cites W2112785261 @default.
• W2148974089 cites W2120785986 @default.
• W2148974089 cites W2120865735 @default.
• W2148974089 cites W2123214754 @default.
• W2148974089 cites W2130979840 @default.
• W2148974089 cites W2131118100 @default.
• W2148974089 cites W2132561013 @default.
• W2148974089 cites W2133211243 @default.
• W2148974089 cites W2133478935 @default.
• W2148974089 cites W2134093135 @default.
• W2148974089 cites W2138954682 @default.
• W2148974089 cites W2140741841 @default.
• W2148974089 cites W2144372599 @default.
• W2148974089 cites W2151922790 @default.
• W2148974089 cites W2156322485 @default.
• W2148974089 cites W2161222316 @default.
• W2148974089 cites W2166179294 @default.
• W2148974089 cites W2166249898 @default.
• W2148974089 cites W2167557255 @default.
• W2148974089 cites W2169658834 @default.
• W2148974089 cites W410897752 @default.
• W2148974089 cites W2142808522 @default.
• W2148974089 doi "https://doi.org/10.1186/1471-2164-10-407" @default.
• W2148974089 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2753550" @default.
• W2148974089 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19715577" @default.
• W2148974089 hasPublicationYear "2009" @default.
• W2148974089 type Work @default.
• W2148974089 sameAs 2148974089 @default.
• W2148974089 citedByCount "281" @default.
• W2148974089 countsByYear W21489740892012 @default.
• W2148974089 countsByYear W21489740892013 @default.
• W2148974089 countsByYear W21489740892014 @default.
• W2148974089 countsByYear W21489740892015 @default.
• W2148974089 countsByYear W21489740892016 @default.
• W2148974089 countsByYear W21489740892017 @default.
• W2148974089 countsByYear W21489740892018 @default.
• W2148974089 countsByYear W21489740892019 @default.
• W2148974089 countsByYear W21489740892020 @default.
• W2148974089 countsByYear W21489740892021 @default.
• W2148974089 countsByYear W21489740892022 @default.
• W2148974089 countsByYear W21489740892023 @default.
• W2148974089 crossrefType "journal-article" @default.
• W2148974089 hasAuthorship W2148974089A5014974894 @default.
• W2148974089 hasAuthorship W2148974089A5024582072 @default.
• W2148974089 hasAuthorship W2148974089A5058832369 @default.
• W2148974089 hasAuthorship W2148974089A5066940107 @default.
• W2148974089 hasBestOaLocation W21489740891 @default.
• W2148974089 hasConcept C104317684 @default.
• W2148974089 hasConcept C105795698 @default.
• W2148974089 hasConcept C143660080 @default.
• W2148974089 hasConcept C145059251 @default.
• W2148974089 hasConcept C150194340 @default.
• W2148974089 hasConcept C153911025 @default.
• W2148974089 hasConcept C18431079 @default.
• W2148974089 hasConcept C185592680 @default.
• W2148974089 hasConcept C186836561 @default.
• W2148974089 hasConcept C187882448 @default.
• W2148974089 hasConcept C2775905019 @default.
• W2148974089 hasConcept C2781162219 @default.
• W2148974089 hasConcept C2781188995 @default.
• W2148974089 hasConcept C33923547 @default.
• W2148974089 hasConcept C43617362 @default.
• W2148974089 hasConcept C48023723 @default.
• W2148974089 hasConcept C54355233 @default.
• W2148974089 hasConcept C70721500 @default.
• W2148974089 hasConcept C86803240 @default.
• W2148974089 hasConcept C95371953 @default.
• W2148974089 hasConcept C9893847 @default.
• W2148974089 hasConceptScore W2148974089C104317684 @default.

• next