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- W2149018829 abstract "Summary Heat shock protein of 70 kDa (HSP70) was revealed in the cells of Amoeba proteus strain Da by immunoblotting with the use of polyand monoclonal antiHSP70 antibodies. This protein can bind ATP and GTP according to ATPagarose chromatography data. The untreated cells contained surprisingly high level of HSP70. The one hour heating of the amoeba culture at 37 °C did not cause the induction of HSP70, but resulted in pronounced decrease of its level in 4 hrs after the heat shock. This phenomenon might be connected with the utilization of HSP70 reserves in stress� conditions to protect sensitive protein structures of the cell. After using the monoclonal antiHSP70 antibodies the increase in the HSP70 content was revealed in 4 hrs after one hour heating of amoeba cells at 32 °Cthe temperature close to the borders of temperature tolerant range (TTR) of Da amoeba strain. It looks like the temperatures causing the induction of HSPs depend upon the strain (species) TTR, presumably even indicating its natural borders." @default.
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- W2149018829 date "2001-01-01" @default.
- W2149018829 modified "2023-09-24" @default.
- W2149018829 title "Heat shock protein of 70 kDa in amoeba proteus" @default.
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