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• W2149021845 abstract "ObjectiveTo evaluate the effect of magnetic-activated cell sorting in cryopreservation–thawing protocols on sperm motility and cryosurvival rate.DesignProspective-controlled study.SettingAndrology department at a university-based medical institution.Patient(s)Ten healthy volunteer sperm donors.Intervention(s)Sperm populations were separated using annexin-V magnetic-activated cell sorting before and after the cryopreservation–thawing process.Main Outcome Measure(s)Sperm motility and cryosurvival rate.Result(s)Annexin-negative sperm separated by magnetic-activated cell sorting had statistically significantly higher motility following cryopreservation–thawing than sperm that were not separated. Similarly, annexin-negative spermatozoa also had higher cryosurvival rate than sperm cryopreserved without magnetic-activated cell sorting and sperm that were annexin-positive.Conclusion(s)Superparamagnetic annexin V-conjugated microbeads can separate spermatozoa with externalized phosphatidylserine, which is considered one of the early features of late apoptosis. The separation of a distinctive population of nonapoptotic spermatozoa with intact membranes may optimize the cryopreservation–thawing outcome. Magnetic-activated cell sorting using annexin-V microbeads enhances sperm motility and cryosurvival rates following cryopreservation. To evaluate the effect of magnetic-activated cell sorting in cryopreservation–thawing protocols on sperm motility and cryosurvival rate. Prospective-controlled study. Andrology department at a university-based medical institution. Ten healthy volunteer sperm donors. Sperm populations were separated using annexin-V magnetic-activated cell sorting before and after the cryopreservation–thawing process. Sperm motility and cryosurvival rate. Annexin-negative sperm separated by magnetic-activated cell sorting had statistically significantly higher motility following cryopreservation–thawing than sperm that were not separated. Similarly, annexin-negative spermatozoa also had higher cryosurvival rate than sperm cryopreserved without magnetic-activated cell sorting and sperm that were annexin-positive. Superparamagnetic annexin V-conjugated microbeads can separate spermatozoa with externalized phosphatidylserine, which is considered one of the early features of late apoptosis. The separation of a distinctive population of nonapoptotic spermatozoa with intact membranes may optimize the cryopreservation–thawing outcome. Magnetic-activated cell sorting using annexin-V microbeads enhances sperm motility and cryosurvival rates following cryopreservation." @default.
• W2149021845 created "2016-06-24" @default.
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• W2149021845 date "2005-05-01" @default.
• W2149021845 modified "2023-09-23" @default.
• W2149021845 title "Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates" @default.
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• W2149021845 doi "https://doi.org/10.1016/j.fertnstert.2004.11.052" @default.
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