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- W2149041246 abstract "The proteomic composition of the Arabidopsis (Arabidopsis thaliana) Golgi apparatus is currently reasonably well documented; however, little is known about the relative abundances between different proteins within this compartment. Accurate quantitative information of Golgi resident proteins is of great importance: it facilitates a better understanding of the biochemical processes that take place within this organelle, especially those of different polysaccharide synthesis pathways. Golgi resident proteins are challenging to quantify because the abundance of this organelle is relatively low within the cell. In this study, an organelle fractionation approach targeting the Golgi apparatus was combined with a label-free quantitative mass spectrometry (data-independent acquisition method using ion mobility separation known as LC-IMS-MSE [or HDMSE]) to simultaneously localize proteins to the Golgi apparatus and assess their relative quantity. In total, 102 Golgi-localized proteins were quantified. These data show that organelle fractionation in conjunction with label-free quantitative mass spectrometry is a powerful and relatively simple tool to access protein organelle localization and their relative abundances. The findings presented open a unique view on the organization of the plant Golgi apparatus, leading toward unique hypotheses centered on the biochemical processes of this organelle." @default.
- W2149041246 created "2016-06-24" @default.
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- W2149041246 creator A5048436688 @default.
- W2149041246 creator A5064560423 @default.
- W2149041246 creator A5073574820 @default.
- W2149041246 date "2014-08-13" @default.
- W2149041246 modified "2023-10-03" @default.
- W2149041246 title "Label-Free Protein Quantification for Plant Golgi Protein Localization and Abundance" @default.
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- W2149041246 doi "https://doi.org/10.1104/pp.114.245589" @default.
- W2149041246 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4213074" @default.
- W2149041246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25122472" @default.
- W2149041246 hasPublicationYear "2014" @default.
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