FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149045044> ?p ?o ?g. }

• W2149045044 endingPage "1171" @default.
• W2149045044 startingPage "1163" @default.
• W2149045044 abstract "Abstract MicroRNAs (miRNAs) play important roles in global gene regulation. Researchers in recombinant protein production have proposed miRNAs as biomarkers and cell engineering targets. However, miRNA expression remains understudied in Chinese Hamster Ovary cells, one of the most commonly used host cell systems for therapeutic protein production. To profile highly conserved miRNA expression, we used the miRCURY™ miRNA array for screening miRNAs in CHO cells. The selection criteria for further miRNA profiling included positive hybridization signals and experimentally validated predicted regulatory targets. On the basis of screening, we selected 16 miRNAs for quantitative RT‐PCR profiling. We profiled miR expression in parental CHO DG44 and CHO K1 cell lines as well as four recombinant DG44‐derived CHO lines producing a recombinant human IgG. We observed that miR‐221 and miR‐222 were significantly downregulated in all IgG‐producing cell lines when compared with parental DG44, whereas miR‐125b was significantly downregulated in one IgG‐producing line. In another IgG‐producing line, miR‐19a was significantly upregulated. miRNA expression was also profiled in two of these lines that were amplified by stepwise increase of methotrexate. In both amplified cell lines, let‐7b and miR‐221 were significantly downregulated. In parental CHO K1, let‐7b, miR‐15b, and miR‐17 were significantly downregulated when compared with DG44. The results reported here are the first steps toward profiling highly conserved miRNAs and studying the clonal difference in miRNA expression in CHO cells and may shed light on using miRNAs in cell engineering. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011" @default.
• W2149045044 created "2016-06-24" @default.
• W2149045044 creator A5008572393 @default.
• W2149045044 creator A5011019803 @default.
• W2149045044 creator A5026146066 @default.
• W2149045044 creator A5030787462 @default.
• W2149045044 creator A5049275905 @default.
• W2149045044 creator A5056567265 @default.
• W2149045044 date "2011-06-20" @default.
• W2149045044 modified "2023-10-10" @default.
• W2149045044 title "Profiling highly conserved microrna expression in recombinant IgG-producing and parental Chinese hamster ovary cells" @default.
• W2149045044 cites W144423133 @default.
• W2149045044 cites W1964731342 @default.
• W2149045044 cites W1975437957 @default.
• W2149045044 cites W1976492411 @default.
• W2149045044 cites W1977920233 @default.
• W2149045044 cites W1981979083 @default.
• W2149045044 cites W1982535740 @default.
• W2149045044 cites W1984935494 @default.
• W2149045044 cites W1985825541 @default.
• W2149045044 cites W1987851658 @default.
• W2149045044 cites W2003633088 @default.
• W2149045044 cites W2005101609 @default.
• W2149045044 cites W2006906242 @default.
• W2149045044 cites W2010848701 @default.
• W2149045044 cites W2015018301 @default.
• W2149045044 cites W2017199818 @default.
• W2149045044 cites W2019148834 @default.
• W2149045044 cites W2019371398 @default.
• W2149045044 cites W2023495875 @default.
• W2149045044 cites W2026468262 @default.
• W2149045044 cites W2026570544 @default.
• W2149045044 cites W2027097496 @default.
• W2149045044 cites W2028994878 @default.
• W2149045044 cites W2030946712 @default.
• W2149045044 cites W2033595939 @default.
• W2149045044 cites W2037631027 @default.
• W2149045044 cites W2060459012 @default.
• W2149045044 cites W2066639891 @default.
• W2149045044 cites W2070571588 @default.
• W2149045044 cites W2074664629 @default.
• W2149045044 cites W2074701970 @default.
• W2149045044 cites W2082657674 @default.
• W2149045044 cites W2084169171 @default.
• W2149045044 cites W2084400739 @default.
• W2149045044 cites W2089039928 @default.
• W2149045044 cites W2089320547 @default.
• W2149045044 cites W2097713515 @default.
• W2149045044 cites W2102144501 @default.
• W2149045044 cites W2108836814 @default.
• W2149045044 cites W2110525565 @default.
• W2149045044 cites W2120686615 @default.
• W2149045044 cites W2123079914 @default.
• W2149045044 cites W2123301882 @default.
• W2149045044 cites W2124268899 @default.
• W2149045044 cites W2125929767 @default.
• W2149045044 cites W2129355962 @default.
• W2149045044 cites W2132194692 @default.
• W2149045044 cites W2138664009 @default.
• W2149045044 cites W2139203062 @default.
• W2149045044 cites W2141128504 @default.
• W2149045044 cites W2149385948 @default.
• W2149045044 cites W2149879897 @default.
• W2149045044 cites W2151868278 @default.
• W2149045044 cites W2151922790 @default.
• W2149045044 cites W2154918321 @default.
• W2149045044 cites W2157754963 @default.
• W2149045044 cites W2160913143 @default.
• W2149045044 cites W2161222316 @default.
• W2149045044 cites W2162224376 @default.
• W2149045044 cites W2162694012 @default.
• W2149045044 cites W2169401756 @default.
• W2149045044 doi "https://doi.org/10.1002/btpr.556" @default.
• W2149045044 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21692195" @default.
• W2149045044 hasPublicationYear "2011" @default.
• W2149045044 type Work @default.
• W2149045044 sameAs 2149045044 @default.
• W2149045044 citedByCount "31" @default.
• W2149045044 countsByYear W21490450442012 @default.
• W2149045044 countsByYear W21490450442013 @default.
• W2149045044 countsByYear W21490450442014 @default.
• W2149045044 countsByYear W21490450442015 @default.
• W2149045044 countsByYear W21490450442016 @default.
• W2149045044 countsByYear W21490450442017 @default.
• W2149045044 countsByYear W21490450442018 @default.
• W2149045044 countsByYear W21490450442019 @default.
• W2149045044 countsByYear W21490450442021 @default.
• W2149045044 countsByYear W21490450442022 @default.
• W2149045044 crossrefType "journal-article" @default.
• W2149045044 hasAuthorship W2149045044A5008572393 @default.
• W2149045044 hasAuthorship W2149045044A5011019803 @default.
• W2149045044 hasAuthorship W2149045044A5026146066 @default.
• W2149045044 hasAuthorship W2149045044A5030787462 @default.
• W2149045044 hasAuthorship W2149045044A5049275905 @default.
• W2149045044 hasAuthorship W2149045044A5056567265 @default.
• W2149045044 hasConcept C104317684 @default.
• W2149045044 hasConcept C145059251 @default.
• W2149045044 hasConcept C150194340 @default.

• next