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- W2149081656 abstract "An electrode-based system capable of monitoring ionized Ca2+ concentrations ([Ca2+]) < 1 μM was used to examine the regulation of extramitochondrial [Ca2+] by rat liver mitochondria. At the point of steady state balance between Ca2+ uptake and release, [Ca2+] ranged between 0.5 and 1.0 μM in a KCl/Hepes/succinate medium. When 1 mM Mg2+ was included in this basal medium, the range of steady state [Ca2+] values was 1–2 μM. Further additions (3 mM MgATP and 2 mM Pi) lowered extramitochondrial [Ca2+] to 0.4–0.8 μM. Thus under experimental conditions simulating the control of cytosolic [Ca2+], liver mitochondria buffered extramitochondrial [Ca2+] at constant values within the range of [Ca2+] estimated for liver cytosol; and cytosolic levels of Mg2+ and ATP significantly affected those steady state [Ca2+] values in directions consistent with previously reported effects of those modulators on mitochondrial Ca2+ uptake and release." @default.
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- W2149081656 date "1980-07-01" @default.
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- W2149081656 title "Steady state regulation of extramitochondrial Ca2+ by rat liver mitochondria. Effects of Mg2+ and ATP" @default.
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- W2149081656 doi "https://doi.org/10.1016/0005-2728(80)90155-3" @default.
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