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• W2149096104 abstract "We have utilized in vitro selection technology to develop allosteric ribozyme sensors that are specific for the small molecule analytes caffeine or aspartame. Caffeine‐ or aspartame‐responsive ribozymes were converted into fluorescence‐based RiboReporter™ sensor systems that were able to detect caffeine or aspartame in solution over a concentration range from 0.5 to 5 mM. With read‐times as short as 5 min, these caffeine‐ or aspartame‐dependent ribozymes function as highly specific and facile molecular sensors. Interestingly, successful isolation of allosteric ribozymes for the analytes described here was enabled by a novel selection strategy that incorporated elements of both modular design and activity‐based selection methods typically used for generation of catalytic nucleic acids." @default.
• W2149096104 created "2016-06-24" @default.
• W2149096104 creator A5029562738 @default.
• W2149096104 date "2004-03-08" @default.
• W2149096104 modified "2023-10-15" @default.
• W2149096104 title "A novel strategy for selection of allosteric ribozymes yields RiboReporterTM sensors for caffeine and aspartame" @default.
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• W2149096104 doi "https://doi.org/10.1093/nar/gkh336" @default.
• W2149096104 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/390333" @default.
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