FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149149145> ?p ?o ?g. }

• W2149149145 endingPage "696" @default.
• W2149149145 startingPage "682" @default.
• W2149149145 abstract "The objective of this study was to isolate osteoprogenitor cells (OPC) from BM mesenchymal stromal cells (MSC) and test their capacity to proliferate and differentiate into osteoblasts.Human MSC were separated on a Percoll gradient and cultured in DMEM supplemented with 15% human serum, and characterized by flow cytometric analyzes for CD34, CD13, CD90, CD105 and CD117. To induce differentiation, cultured cells were exposed to 10(-7) m dexamethasone (dexa) and/or 10(-3) m sodium beta-glycerophosphate (beta-GlyP) and 1,25-dihydroxyvitamin D3 (calcitriol) or 9-cis-retinoic acid (9-RA).alkaline phosphatase (AP) activity was detected in cells irrespective of the dexa and/or beta-GlyP treatment. Antigenic phenotypes of MSC were CD34- (more than 99%) and CD13+ CD90+ CD105+ CD117+ (c. 50%). The treatment induced extracellular calcium deposition and gene and protein expression of osteonectin (ON) and bone sialoprotein (BSP): beta-GlyP induced an increase (c. 2.2-fold) of the ON gene and dexa augmented (c. 2.7-fold) the gene expression of BSP II. Gene expression of BSP I reached a maximum at 3 weeks of combined treatment. Osteocalcin gene expression was induced only after additional treatment with calcitriol or 9-RA. Ultrastructural analysis revealed the secretory phenotype of OPC.Under appropriate treatment, MSC can give rise to OPC that have the capacity to differentiate into osteoblasts characterized by the expression of osteogenic markers, osteoblastic properties and stromal BM cells phenotypes. These cells may represent a promising material to be utilized in orthopedic cellular therapy." @default.
• W2149149145 created "2016-06-24" @default.
• W2149149145 creator A5002220204 @default.
• W2149149145 creator A5009364404 @default.
• W2149149145 creator A5025731571 @default.
• W2149149145 creator A5028981837 @default.
• W2149149145 creator A5033828684 @default.
• W2149149145 creator A5038479199 @default.
• W2149149145 creator A5053769569 @default.
• W2149149145 creator A5085493978 @default.
• W2149149145 date "2007-01-01" @default.
• W2149149145 modified "2023-09-30" @default.
• W2149149145 title "Proliferation, differentiation and characterization of osteoblasts from human BM mesenchymal cells" @default.
• W2149149145 cites W1965953554 @default.
• W2149149145 cites W1969281312 @default.
• W2149149145 cites W1974766733 @default.
• W2149149145 cites W1982812308 @default.
• W2149149145 cites W1993640536 @default.
• W2149149145 cites W1999242737 @default.
• W2149149145 cites W2004833467 @default.
• W2149149145 cites W2005097199 @default.
• W2149149145 cites W2010143584 @default.
• W2149149145 cites W2035087930 @default.
• W2149149145 cites W2049769334 @default.
• W2149149145 cites W2053976497 @default.
• W2149149145 cites W2062076773 @default.
• W2149149145 cites W2067872631 @default.
• W2149149145 cites W2072047772 @default.
• W2149149145 cites W2074549856 @default.
• W2149149145 cites W2086819446 @default.
• W2149149145 cites W2091087456 @default.
• W2149149145 cites W2095257528 @default.
• W2149149145 cites W2126517599 @default.
• W2149149145 cites W2145816248 @default.
• W2149149145 cites W2155226427 @default.
• W2149149145 cites W2159999899 @default.
• W2149149145 cites W2791458743 @default.
• W2149149145 cites W4211245603 @default.
• W2149149145 cites W4298879297 @default.
• W2149149145 doi "https://doi.org/10.1080/14653240701561329" @default.
• W2149149145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17917883" @default.
• W2149149145 hasPublicationYear "2007" @default.
• W2149149145 type Work @default.
• W2149149145 sameAs 2149149145 @default.
• W2149149145 citedByCount "59" @default.
• W2149149145 countsByYear W21491491452012 @default.
• W2149149145 countsByYear W21491491452013 @default.
• W2149149145 countsByYear W21491491452014 @default.
• W2149149145 countsByYear W21491491452015 @default.
• W2149149145 countsByYear W21491491452016 @default.
• W2149149145 countsByYear W21491491452017 @default.
• W2149149145 countsByYear W21491491452018 @default.
• W2149149145 countsByYear W21491491452020 @default.
• W2149149145 countsByYear W21491491452021 @default.
• W2149149145 countsByYear W21491491452022 @default.
• W2149149145 crossrefType "journal-article" @default.
• W2149149145 hasAuthorship W2149149145A5002220204 @default.
• W2149149145 hasAuthorship W2149149145A5009364404 @default.
• W2149149145 hasAuthorship W2149149145A5025731571 @default.
• W2149149145 hasAuthorship W2149149145A5028981837 @default.
• W2149149145 hasAuthorship W2149149145A5033828684 @default.
• W2149149145 hasAuthorship W2149149145A5038479199 @default.
• W2149149145 hasAuthorship W2149149145A5053769569 @default.
• W2149149145 hasAuthorship W2149149145A5085493978 @default.
• W2149149145 hasConcept C10205521 @default.
• W2149149145 hasConcept C116716535 @default.
• W2149149145 hasConcept C124490489 @default.
• W2149149145 hasConcept C131075544 @default.
• W2149149145 hasConcept C134018914 @default.
• W2149149145 hasConcept C153911025 @default.
• W2149149145 hasConcept C160160445 @default.
• W2149149145 hasConcept C16930146 @default.
• W2149149145 hasConcept C181199279 @default.
• W2149149145 hasConcept C185592680 @default.
• W2149149145 hasConcept C198826908 @default.
• W2149149145 hasConcept C202751555 @default.
• W2149149145 hasConcept C2776079296 @default.
• W2149149145 hasConcept C2776432469 @default.
• W2149149145 hasConcept C2778260815 @default.
• W2149149145 hasConcept C2781460891 @default.
• W2149149145 hasConcept C28328180 @default.
• W2149149145 hasConcept C502942594 @default.
• W2149149145 hasConcept C55493867 @default.
• W2149149145 hasConcept C86803240 @default.
• W2149149145 hasConcept C95444343 @default.
• W2149149145 hasConceptScore W2149149145C10205521 @default.
• W2149149145 hasConceptScore W2149149145C116716535 @default.
• W2149149145 hasConceptScore W2149149145C124490489 @default.
• W2149149145 hasConceptScore W2149149145C131075544 @default.
• W2149149145 hasConceptScore W2149149145C134018914 @default.
• W2149149145 hasConceptScore W2149149145C153911025 @default.
• W2149149145 hasConceptScore W2149149145C160160445 @default.
• W2149149145 hasConceptScore W2149149145C16930146 @default.
• W2149149145 hasConceptScore W2149149145C181199279 @default.
• W2149149145 hasConceptScore W2149149145C185592680 @default.
• W2149149145 hasConceptScore W2149149145C198826908 @default.
• W2149149145 hasConceptScore W2149149145C202751555 @default.
• W2149149145 hasConceptScore W2149149145C2776079296 @default.

• next