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- W2149164489 abstract "We constructed two aptamers, each of which contains a 7-nt-long loop complementary to the anticodon loop of a suppressor tRNA. One of these aptamers can form a stable bimolecular complex with the suppressor tRNA in vitro and protects the 7 nt in the suppressor's anticodon loop from RNase S1. An Escherichia coli strain, carrying an amber mutation in the lac Z gene, produces beta-galactosidase only if the suppressor is present; the aptamer's coexpression in the cell inhibits the activity of the suppressor tRNA. Moreover, in E. coli extract, the aptamer partially inhibits the read-through of the stop codon on the part of the suppressor tRNA. These results point to a novel strategy that need not be limited to the suppressor tRNA. By constructing appropriate inducible aptamers, it may well be possible to effectively control translation in vivo." @default.
- W2149164489 created "2016-06-24" @default.
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- W2149164489 date "2002-07-01" @default.
- W2149164489 modified "2023-09-25" @default.
- W2149164489 title "Trans-acting RNA inhibits tRNA suppressor activity in vivo" @default.
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- W2149164489 doi "https://doi.org/10.1017/s1355838202026031" @default.
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