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- W2149165061 abstract "An important question in protein folding is whether compact substructures or domains are autonomous units of folding and assembly. The protomer of the tetrameric D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima has a complex coenzyme-binding domain, in which residues 1-146 form a compact substructure with the last 31 residues (313-333). Here it is shown that the gene of a single-chain protein can be expressed in Escherichia coli after deleting the 163 codons corresponding to the interspersed catalytic domain (150-312). The purified gene product is a soluble, monomeric protein that binds both NAD+ and NADH strongly and possesses the same unfolding transition induced by guanidinium chloride as the native tetramer. The autonomous folding of the coenzyme-binding domain has interesting implications for the folding, assembly, function, and evolution of the native enzyme." @default.
- W2149165061 created "2016-06-24" @default.
- W2149165061 creator A5023088787 @default.
- W2149165061 creator A5027224579 @default.
- W2149165061 creator A5033878382 @default.
- W2149165061 creator A5069635640 @default.
- W2149165061 date "1994-03-01" @default.
- W2149165061 modified "2023-10-18" @default.
- W2149165061 title "Autonomous folding of the excised coenzyme-binding domain of D-glyceraldehyde 3-phosphate dehydrogenase from Thermotoga maritima" @default.
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- W2149165061 doi "https://doi.org/10.1002/pro.5560030305" @default.
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