FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149187876> ?p ?o ?g. }

• W2149187876 endingPage "519" @default.
• W2149187876 startingPage "509" @default.
• W2149187876 abstract "The nuclear import of proteins larger than Mr 40,000 depends on the presence of a nuclear localization signal (NLS) corresponding either to a short peptide sequence or to defined sugars. The sugar-dependent nuclear import was previously evidenced by using glycosylated proteins (neoglycoproteins) introduced into the cytosol of cells either by electroporation or on digitonin-permeabilization and was shown to be distinct from the peptide NLS-mediated pathway. In this work, we used a microinjection approach to compare the two nuclear import pathways in intact living cells. The intracellular localization of fluorescent NLS-BSA or Glc-BSA injected into the cytosol was analyzed by confocal microscopy. Novel differences between the two mechanisms were evidenced. First, Glc-BSA migrated less efficiently into the nucleus than NLS-BSA because of a cytosolic retention. Second, the import of neoglycoproteins was not affected by microinjection of antinuclear import factor importin/karyopherin beta antibodies, whereas the NLS-dependent transport was completely abolished. Third, the nuclear import activity of Glc-BSA was found to be cell cycle-dependent in thymidine and hydroxyurea-treated HeLa cells, with greatest efficiency during G1/S transition and S phases, whereas NLS-BSA was imported with the same efficiency during any stage of the cell cycle but the G2 phase. Fourth, we show that after mitosis, nonglycosylated BSA was excluded from the nucleus contrary to Glc-BSA. In both cases, the nuclear import signals (NLS or alpha-glucoside) were grafted onto BSA; such tools led to a clear-cut conclusion, which will reach a full physiological significance when they are confirmed in the case of endogenous (glyco)proteins." @default.
• W2149187876 created "2016-06-24" @default.
• W2149187876 creator A5057499544 @default.
• W2149187876 creator A5071251708 @default.
• W2149187876 creator A5082117704 @default.
• W2149187876 creator A5083306009 @default.
• W2149187876 date "2003-04-02" @default.
• W2149187876 modified "2023-09-25" @default.
• W2149187876 title "Sugar-dependent nuclear import of glycosylated proteins in living cells" @default.
• W2149187876 cites W1570986231 @default.
• W2149187876 cites W1572212600 @default.
• W2149187876 cites W1588589182 @default.
• W2149187876 cites W1603783081 @default.
• W2149187876 cites W163275813 @default.
• W2149187876 cites W1664376284 @default.
• W2149187876 cites W1691646448 @default.
• W2149187876 cites W1973052124 @default.
• W2149187876 cites W1973073454 @default.
• W2149187876 cites W1974343165 @default.
• W2149187876 cites W1974509084 @default.
• W2149187876 cites W1978610450 @default.
• W2149187876 cites W1978798894 @default.
• W2149187876 cites W1979906352 @default.
• W2149187876 cites W1981198355 @default.
• W2149187876 cites W1983850779 @default.
• W2149187876 cites W1984126072 @default.
• W2149187876 cites W1993496373 @default.
• W2149187876 cites W1995147267 @default.
• W2149187876 cites W1997044852 @default.
• W2149187876 cites W2002215739 @default.
• W2149187876 cites W2008580521 @default.
• W2149187876 cites W2014114236 @default.
• W2149187876 cites W2015646338 @default.
• W2149187876 cites W2016904689 @default.
• W2149187876 cites W2019894624 @default.
• W2149187876 cites W2023574559 @default.
• W2149187876 cites W2023680254 @default.
• W2149187876 cites W2024735180 @default.
• W2149187876 cites W2030187415 @default.
• W2149187876 cites W2030264105 @default.
• W2149187876 cites W2035927739 @default.
• W2149187876 cites W2036931498 @default.
• W2149187876 cites W2038313388 @default.
• W2149187876 cites W2039863802 @default.
• W2149187876 cites W2039958767 @default.
• W2149187876 cites W2044532151 @default.
• W2149187876 cites W2048679863 @default.
• W2149187876 cites W2053796273 @default.
• W2149187876 cites W2056026749 @default.
• W2149187876 cites W2056571665 @default.
• W2149187876 cites W2060088582 @default.
• W2149187876 cites W2063385900 @default.
• W2149187876 cites W2066987191 @default.
• W2149187876 cites W2067671009 @default.
• W2149187876 cites W2069355785 @default.
• W2149187876 cites W2071078544 @default.
• W2149187876 cites W2077543321 @default.
• W2149187876 cites W2082949426 @default.
• W2149187876 cites W2085677500 @default.
• W2149187876 cites W2091067750 @default.
• W2149187876 cites W2101752117 @default.
• W2149187876 cites W2128788229 @default.
• W2149187876 cites W2132672808 @default.
• W2149187876 cites W2134025840 @default.
• W2149187876 cites W2134263455 @default.
• W2149187876 cites W2139334062 @default.
• W2149187876 cites W2141529118 @default.
• W2149187876 cites W2143566830 @default.
• W2149187876 cites W2164019525 @default.
• W2149187876 cites W2164931904 @default.
• W2149187876 cites W2164933850 @default.
• W2149187876 cites W2344038510 @default.
• W2149187876 cites W2416336658 @default.
• W2149187876 cites W27872685 @default.
• W2149187876 doi "https://doi.org/10.1093/glycob/cwg064" @default.
• W2149187876 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12672698" @default.
• W2149187876 hasPublicationYear "2003" @default.
• W2149187876 type Work @default.
• W2149187876 sameAs 2149187876 @default.
• W2149187876 citedByCount "38" @default.
• W2149187876 countsByYear W21491878762012 @default.
• W2149187876 countsByYear W21491878762013 @default.
• W2149187876 countsByYear W21491878762014 @default.
• W2149187876 countsByYear W21491878762016 @default.
• W2149187876 countsByYear W21491878762017 @default.
• W2149187876 countsByYear W21491878762019 @default.
• W2149187876 countsByYear W21491878762020 @default.
• W2149187876 countsByYear W21491878762021 @default.
• W2149187876 countsByYear W21491878762022 @default.
• W2149187876 crossrefType "journal-article" @default.
• W2149187876 hasAuthorship W2149187876A5057499544 @default.
• W2149187876 hasAuthorship W2149187876A5071251708 @default.
• W2149187876 hasAuthorship W2149187876A5082117704 @default.
• W2149187876 hasAuthorship W2149187876A5083306009 @default.
• W2149187876 hasConcept C104317684 @default.
• W2149187876 hasConcept C133882971 @default.
• W2149187876 hasConcept C181199279 @default.
• W2149187876 hasConcept C185592680 @default.

• next