Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149248265> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2149248265 abstract "Medulloblastoma is the most common central nervous system tumor in childhood. Despite high survival rates (over 50% for most protocols) the treatment for this tumor usually results in severe sequels. Still, in patients younger than 3 years-old radiotherapy cannot be used turning the prognosis even worse. Polo-like kinase 1 (PLK1), a serine-threonine kinase that regulates mitosis progression, has been repeatedly associated with increased cell proliferation and poorer prognosis in several types of cancer. The aim of this study was to evaluate the effects of the inhibition of PLK1 with BI 2536 on mitotic index clonogenic capacity and cell proliferation, on the UW402 and UW473 pediatric medulloblastoma cell lines. Equal numbers of cells were treated with 50, 100 and 150 nM of BI 2536 for 24, 48 and 72 hours. To calculate the mitotic index cells after treatment dropped onto glass slides, allowed to air dry and stained with Giemsa, 1000 cells were counted. To calculated the clonogenic capacity cells were treated, let to grow for 7 days and then the colonies were fixed with methanol and stained with Giemsa. Only colonies with > 50 cells were counted. To analyze the proliferation cells after treatment were let with culture medium containing 10 μL of XTT dye (XTT II; Roche Molecular Biochemicals, Indianapolis, IN), plates were incubated for 2 hours at 37°C and the formazan product was measured at 450 nm by using an iMark microplate reader (Bio-Rad Laboratories). Each experiment was performed in triplicate wells and repeated in three sets of tests. The tests were analyzed by oneway ANOVA followed by Home-Sidak test. Values of p≤0.05 were considered statistically significant. The Inhibition of PLK1 by this drug showed an efficient decrease (p Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2011 Nov 12-16; San Francisco, CA. Philadelphia (PA): AACR; Mol Cancer Ther 2011;10(11 Suppl):Abstract nr C195." @default.
- W2149248265 created "2016-06-24" @default.
- W2149248265 creator A5013080591 @default.
- W2149248265 creator A5027221850 @default.
- W2149248265 creator A5037226407 @default.
- W2149248265 creator A5053517791 @default.
- W2149248265 creator A5071063454 @default.
- W2149248265 creator A5087411930 @default.
- W2149248265 creator A5089350518 @default.
- W2149248265 date "2011-11-12" @default.
- W2149248265 modified "2023-09-27" @default.
- W2149248265 title "Abstract C195: Inhibition of polo-like kinase 1 (PLK1) by BI 2536: Effects on mitotic index, clonogenic capacity, and proliferation in medulloblastoma cell lines." @default.
- W2149248265 doi "https://doi.org/10.1158/1535-7163.targ-11-c195" @default.
- W2149248265 hasPublicationYear "2011" @default.
- W2149248265 type Work @default.
- W2149248265 sameAs 2149248265 @default.
- W2149248265 citedByCount "0" @default.
- W2149248265 crossrefType "proceedings-article" @default.
- W2149248265 hasAuthorship W2149248265A5013080591 @default.
- W2149248265 hasAuthorship W2149248265A5027221850 @default.
- W2149248265 hasAuthorship W2149248265A5037226407 @default.
- W2149248265 hasAuthorship W2149248265A5053517791 @default.
- W2149248265 hasAuthorship W2149248265A5071063454 @default.
- W2149248265 hasAuthorship W2149248265A5087411930 @default.
- W2149248265 hasAuthorship W2149248265A5089350518 @default.
- W2149248265 hasConcept C117262875 @default.
- W2149248265 hasConcept C142724271 @default.
- W2149248265 hasConcept C146276462 @default.
- W2149248265 hasConcept C1491633281 @default.
- W2149248265 hasConcept C153911025 @default.
- W2149248265 hasConcept C154488023 @default.
- W2149248265 hasConcept C184235292 @default.
- W2149248265 hasConcept C185592680 @default.
- W2149248265 hasConcept C2780789225 @default.
- W2149248265 hasConcept C29537977 @default.
- W2149248265 hasConcept C502942594 @default.
- W2149248265 hasConcept C54355233 @default.
- W2149248265 hasConcept C55493867 @default.
- W2149248265 hasConcept C62112901 @default.
- W2149248265 hasConcept C66640590 @default.
- W2149248265 hasConcept C71924100 @default.
- W2149248265 hasConcept C81885089 @default.
- W2149248265 hasConcept C86803240 @default.
- W2149248265 hasConcept C93304396 @default.
- W2149248265 hasConcept C95444343 @default.
- W2149248265 hasConceptScore W2149248265C117262875 @default.
- W2149248265 hasConceptScore W2149248265C142724271 @default.
- W2149248265 hasConceptScore W2149248265C146276462 @default.
- W2149248265 hasConceptScore W2149248265C1491633281 @default.
- W2149248265 hasConceptScore W2149248265C153911025 @default.
- W2149248265 hasConceptScore W2149248265C154488023 @default.
- W2149248265 hasConceptScore W2149248265C184235292 @default.
- W2149248265 hasConceptScore W2149248265C185592680 @default.
- W2149248265 hasConceptScore W2149248265C2780789225 @default.
- W2149248265 hasConceptScore W2149248265C29537977 @default.
- W2149248265 hasConceptScore W2149248265C502942594 @default.
- W2149248265 hasConceptScore W2149248265C54355233 @default.
- W2149248265 hasConceptScore W2149248265C55493867 @default.
- W2149248265 hasConceptScore W2149248265C62112901 @default.
- W2149248265 hasConceptScore W2149248265C66640590 @default.
- W2149248265 hasConceptScore W2149248265C71924100 @default.
- W2149248265 hasConceptScore W2149248265C81885089 @default.
- W2149248265 hasConceptScore W2149248265C86803240 @default.
- W2149248265 hasConceptScore W2149248265C93304396 @default.
- W2149248265 hasConceptScore W2149248265C95444343 @default.
- W2149248265 hasLocation W21492482651 @default.
- W2149248265 hasOpenAccess W2149248265 @default.
- W2149248265 hasPrimaryLocation W21492482651 @default.
- W2149248265 hasRelatedWork W1907773671 @default.
- W2149248265 hasRelatedWork W2016544926 @default.
- W2149248265 hasRelatedWork W2021188836 @default.
- W2149248265 hasRelatedWork W2076036063 @default.
- W2149248265 hasRelatedWork W2088377303 @default.
- W2149248265 hasRelatedWork W2322465787 @default.
- W2149248265 hasRelatedWork W2333695010 @default.
- W2149248265 hasRelatedWork W2340301086 @default.
- W2149248265 hasRelatedWork W2354990243 @default.
- W2149248265 hasRelatedWork W2363951514 @default.
- W2149248265 hasRelatedWork W2387637841 @default.
- W2149248265 hasRelatedWork W2537623897 @default.
- W2149248265 hasRelatedWork W2571064480 @default.
- W2149248265 hasRelatedWork W301154954 @default.
- W2149248265 hasRelatedWork W3030251567 @default.
- W2149248265 hasRelatedWork W3081551390 @default.
- W2149248265 hasRelatedWork W3107316156 @default.
- W2149248265 hasRelatedWork W3127781201 @default.
- W2149248265 hasRelatedWork W3128819217 @default.
- W2149248265 hasRelatedWork W3142647289 @default.
- W2149248265 isParatext "false" @default.
- W2149248265 isRetracted "false" @default.
- W2149248265 magId "2149248265" @default.
- W2149248265 workType "article" @default.