FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149277409> ?p ?o ?g. }

• W2149277409 abstract "The protein kinase C (PKC) is a family of serine/threonine kinases that consists of 12 different isoforms. Since PKC isoform expressions are known to be specific for different cell types and postnatal developmental stages, we aimed to determine immunolocalizations and protein expression levels of different PKC isoforms in pre-pubertal, pubertal and adult mouse ovaries.Ovaries were obtained from postnatal day 1 (PND1) and PND7 of pre-pubertal, PND21 of pubertal and PND60 of adult mice. Immunolocalizations of PKCα, PKCδ and PKCε isoforms were determined and immunostainings in different cellular components of all follicular stages were evaluated by H-Score. PKCα, PKCδ and PKCε protein expression levels were determined by Western blot. The bands were quantified via ImageJ software. The data obtained from H-Score and ImageJ evaluations were analyzed by ANOVA statistical test.PKCα immunostainings were more intense in oocytes when compared to granulosa and theca cells at different follicular stages of all groups. The Western blot analysis revealed that PKCα expression was significantly higher in PND60 adult ovaries. Conversely, PKCδ immunostainings were more intense in granulosa cells. According to the Western blot analysis, PKCδ protein expression was also higher in PND60 and significantly lower in PND1 ovaries. PKCε immunostaining was more apparent in oocytes. PKCε protein expression was significantly higher in adult PND60 and pubertal PND21 ovaries when compared to pre-pubertal PND7 and PND1 ovaries. Interestingly, PKCε immunostaining was significantly higher in primordial follicles, though PKCα and PKCδ immunostainings were more apparent in larger follicles. PKCα immunostainings of corpora lutea (CL) were significantly higher when compared to follicles in PND60 ovaries.This study demonstrates that PKCα, PKCδ and PKCε isoforms are differentially expressed in particular cellular components of pre-pubertal, pubertal and adult mouse ovarian follicles. Therefore, we suggest that each PKC isoform has unique functions that are controlled by gonadotropin dependent mechanisms during follicular growth, oocyte maturation, ovulation and luteinization." @default.
• W2149277409 created "2016-06-24" @default.
• W2149277409 creator A5016507162 @default.
• W2149277409 creator A5042698061 @default.
• W2149277409 creator A5063406168 @default.
• W2149277409 date "2014-12-01" @default.
• W2149277409 modified "2023-09-26" @default.
• W2149277409 title "Protein kinase C isoforms α, δ and ε are differentially expressed in mouse ovaries at different stages of postnatal development" @default.
• W2149277409 cites W1482208423 @default.
• W2149277409 cites W148785554 @default.
• W2149277409 cites W1775749144 @default.
• W2149277409 cites W1964716747 @default.
• W2149277409 cites W1966221092 @default.
• W2149277409 cites W1976129687 @default.
• W2149277409 cites W1977239487 @default.
• W2149277409 cites W1979374003 @default.
• W2149277409 cites W1981676152 @default.
• W2149277409 cites W2002079988 @default.
• W2149277409 cites W2013022584 @default.
• W2149277409 cites W2016460881 @default.
• W2149277409 cites W2023913804 @default.
• W2149277409 cites W2030754137 @default.
• W2149277409 cites W2034133369 @default.
• W2149277409 cites W2041595247 @default.
• W2149277409 cites W2043052985 @default.
• W2149277409 cites W2048131740 @default.
• W2149277409 cites W2058177903 @default.
• W2149277409 cites W2058374230 @default.
• W2149277409 cites W2078322680 @default.
• W2149277409 cites W2080701638 @default.
• W2149277409 cites W2086524338 @default.
• W2149277409 cites W2090351541 @default.
• W2149277409 cites W2095849729 @default.
• W2149277409 cites W2096323238 @default.
• W2149277409 cites W2099987623 @default.
• W2149277409 cites W2101398935 @default.
• W2149277409 cites W2105486478 @default.
• W2149277409 cites W2113651151 @default.
• W2149277409 cites W2114372808 @default.
• W2149277409 cites W2120810059 @default.
• W2149277409 cites W2121204029 @default.
• W2149277409 cites W2133552234 @default.
• W2149277409 cites W2137586637 @default.
• W2149277409 cites W2138208797 @default.
• W2149277409 cites W2147608564 @default.
• W2149277409 cites W2158975304 @default.
• W2149277409 cites W2234669619 @default.
• W2149277409 cites W4251951766 @default.
• W2149277409 doi "https://doi.org/10.1186/s13048-014-0117-z" @default.
• W2149277409 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4271327" @default.
• W2149277409 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25491605" @default.
• W2149277409 hasPublicationYear "2014" @default.
• W2149277409 type Work @default.
• W2149277409 sameAs 2149277409 @default.
• W2149277409 citedByCount "5" @default.
• W2149277409 countsByYear W21492774092018 @default.
• W2149277409 countsByYear W21492774092019 @default.
• W2149277409 countsByYear W21492774092022 @default.
• W2149277409 countsByYear W21492774092023 @default.
• W2149277409 crossrefType "journal-article" @default.
• W2149277409 hasAuthorship W2149277409A5016507162 @default.
• W2149277409 hasAuthorship W2149277409A5042698061 @default.
• W2149277409 hasAuthorship W2149277409A5063406168 @default.
• W2149277409 hasBestOaLocation W21492774091 @default.
• W2149277409 hasConcept C104317684 @default.
• W2149277409 hasConcept C126322002 @default.
• W2149277409 hasConcept C134018914 @default.
• W2149277409 hasConcept C16685009 @default.
• W2149277409 hasConcept C184235292 @default.
• W2149277409 hasConcept C187785154 @default.
• W2149277409 hasConcept C195794163 @default.
• W2149277409 hasConcept C204232928 @default.
• W2149277409 hasConcept C2776415932 @default.
• W2149277409 hasConcept C4224716 @default.
• W2149277409 hasConcept C53345823 @default.
• W2149277409 hasConcept C55493867 @default.
• W2149277409 hasConcept C71924100 @default.
• W2149277409 hasConcept C86803240 @default.
• W2149277409 hasConcept C95444343 @default.
• W2149277409 hasConceptScore W2149277409C104317684 @default.
• W2149277409 hasConceptScore W2149277409C126322002 @default.
• W2149277409 hasConceptScore W2149277409C134018914 @default.
• W2149277409 hasConceptScore W2149277409C16685009 @default.
• W2149277409 hasConceptScore W2149277409C184235292 @default.
• W2149277409 hasConceptScore W2149277409C187785154 @default.
• W2149277409 hasConceptScore W2149277409C195794163 @default.
• W2149277409 hasConceptScore W2149277409C204232928 @default.
• W2149277409 hasConceptScore W2149277409C2776415932 @default.
• W2149277409 hasConceptScore W2149277409C4224716 @default.
• W2149277409 hasConceptScore W2149277409C53345823 @default.
• W2149277409 hasConceptScore W2149277409C55493867 @default.
• W2149277409 hasConceptScore W2149277409C71924100 @default.
• W2149277409 hasConceptScore W2149277409C86803240 @default.
• W2149277409 hasConceptScore W2149277409C95444343 @default.
• W2149277409 hasIssue "1" @default.
• W2149277409 hasLocation W21492774091 @default.
• W2149277409 hasLocation W21492774092 @default.
• W2149277409 hasLocation W21492774093 @default.
• W2149277409 hasLocation W21492774094 @default.
• W2149277409 hasOpenAccess W2149277409 @default.

• next