FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149291385> ?p ?o ?g. }

• W2149291385 endingPage "3575" @default.
• W2149291385 startingPage "3563" @default.
• W2149291385 abstract "While there has been considerable progress in designing protein–protein interactions, the design of proteins that bind polar surfaces is an unmet challenge. We describe the computational design of a protein that binds the acidic active site of hen egg lysozyme and inhibits the enzyme. The design process starts with two polar amino acids that fit deep into the enzyme active site, identifies a protein scaffold that supports these residues and is complementary in shape to the lysozyme active-site region, and finally optimizes the surrounding contact surface for high-affinity binding. Following affinity maturation, a protein designed using this method bound lysozyme with low nanomolar affinity, and a combination of NMR studies, crystallography, and knockout mutagenesis confirmed the designed binding surface and orientation. Saturation mutagenesis with selection and deep sequencing demonstrated that specific designed interactions extending well beyond the centrally grafted polar residues are critical for high-affinity binding." @default.
• W2149291385 created "2016-06-24" @default.
• W2149291385 creator A5004904484 @default.
• W2149291385 creator A5007184616 @default.
• W2149291385 creator A5010061026 @default.
• W2149291385 creator A5031768744 @default.
• W2149291385 creator A5032900194 @default.
• W2149291385 creator A5041462222 @default.
• W2149291385 creator A5041481754 @default.
• W2149291385 creator A5044462901 @default.
• W2149291385 creator A5044542421 @default.
• W2149291385 creator A5053192926 @default.
• W2149291385 creator A5076705302 @default.
• W2149291385 creator A5088663494 @default.
• W2149291385 date "2013-09-01" @default.
• W2149291385 modified "2023-10-06" @default.
• W2149291385 title "Computational Design of a Protein-Based Enzyme Inhibitor" @default.
• W2149291385 cites W159622809 @default.
• W2149291385 cites W1681479328 @default.
• W2149291385 cites W1969644422 @default.
• W2149291385 cites W1987560617 @default.
• W2149291385 cites W1990789777 @default.
• W2149291385 cites W1995017064 @default.
• W2149291385 cites W1996112440 @default.
• W2149291385 cites W2007804411 @default.
• W2149291385 cites W2010753961 @default.
• W2149291385 cites W2015526636 @default.
• W2149291385 cites W2018295245 @default.
• W2149291385 cites W2021256658 @default.
• W2149291385 cites W2029562819 @default.
• W2149291385 cites W2049275721 @default.
• W2149291385 cites W2051710717 @default.
• W2149291385 cites W2053134976 @default.
• W2149291385 cites W2053937911 @default.
• W2149291385 cites W2054392131 @default.
• W2149291385 cites W2058527555 @default.
• W2149291385 cites W2065654184 @default.
• W2149291385 cites W2073338313 @default.
• W2149291385 cites W2083445427 @default.
• W2149291385 cites W2083945806 @default.
• W2149291385 cites W2084493621 @default.
• W2149291385 cites W2093666200 @default.
• W2149291385 cites W2097493124 @default.
• W2149291385 cites W2111387452 @default.
• W2149291385 cites W2112130467 @default.
• W2149291385 cites W2122092772 @default.
• W2149291385 cites W2123850444 @default.
• W2149291385 cites W2129202771 @default.
• W2149291385 cites W2136206140 @default.
• W2149291385 cites W2138905138 @default.
• W2149291385 cites W2146773519 @default.
• W2149291385 cites W2148895983 @default.
• W2149291385 cites W2151460035 @default.
• W2149291385 cites W2151887741 @default.
• W2149291385 cites W2152902370 @default.
• W2149291385 cites W2158550010 @default.
• W2149291385 cites W2201713963 @default.
• W2149291385 doi "https://doi.org/10.1016/j.jmb.2013.06.035" @default.
• W2149291385 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3818146" @default.
• W2149291385 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23827138" @default.
• W2149291385 hasPublicationYear "2013" @default.
• W2149291385 type Work @default.
• W2149291385 sameAs 2149291385 @default.
• W2149291385 citedByCount "87" @default.
• W2149291385 countsByYear W21492913852013 @default.
• W2149291385 countsByYear W21492913852014 @default.
• W2149291385 countsByYear W21492913852015 @default.
• W2149291385 countsByYear W21492913852016 @default.
• W2149291385 countsByYear W21492913852017 @default.
• W2149291385 countsByYear W21492913852018 @default.
• W2149291385 countsByYear W21492913852019 @default.
• W2149291385 countsByYear W21492913852020 @default.
• W2149291385 countsByYear W21492913852021 @default.
• W2149291385 countsByYear W21492913852022 @default.
• W2149291385 countsByYear W21492913852023 @default.
• W2149291385 crossrefType "journal-article" @default.
• W2149291385 hasAuthorship W2149291385A5004904484 @default.
• W2149291385 hasAuthorship W2149291385A5007184616 @default.
• W2149291385 hasAuthorship W2149291385A5010061026 @default.
• W2149291385 hasAuthorship W2149291385A5031768744 @default.
• W2149291385 hasAuthorship W2149291385A5032900194 @default.
• W2149291385 hasAuthorship W2149291385A5041462222 @default.
• W2149291385 hasAuthorship W2149291385A5041481754 @default.
• W2149291385 hasAuthorship W2149291385A5044462901 @default.
• W2149291385 hasAuthorship W2149291385A5044542421 @default.
• W2149291385 hasAuthorship W2149291385A5053192926 @default.
• W2149291385 hasAuthorship W2149291385A5076705302 @default.
• W2149291385 hasAuthorship W2149291385A5088663494 @default.
• W2149291385 hasBestOaLocation W21492913852 @default.
• W2149291385 hasConcept C103408237 @default.
• W2149291385 hasConcept C104317684 @default.
• W2149291385 hasConcept C107824862 @default.
• W2149291385 hasConcept C109129352 @default.
• W2149291385 hasConcept C143065580 @default.
• W2149291385 hasConcept C147816474 @default.
• W2149291385 hasConcept C152769699 @default.
• W2149291385 hasConcept C16318435 @default.
• W2149291385 hasConcept C181199279 @default.

• next