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• W2149292647 abstract "Ovaries were surgically removed from female goats (Toggenburg, Nubian and Saanen breeds). Oocytes were collected by follicular aspiration or after ovaries were minced, then matured in mTCM-199 with 100 μg LH + 0.5 μg FSH + 1.0 μg estradiol 17-β/ml for 27 h prior to in vitro fertilization (17). Although more oocytes were made available by mincing than by aspiration, higher proportions of aspirated oocytes were fertilized and developed to morulae. Proportions that fertilized and reached morulae were 82102 (80.4%) and 50102 (49.0%) versus 77126 (61.1%) and 27126 (21.4%) for oocytes obtained by aspiration and after ovarian mincing, respectively (P<0.05). Proportions of inseminated ova undergoing cleavage and continuing development to the morula stage differed significantly (P<0.05) among 5 co-culture treatment groups, with higher proportions of cleavage (2327, 85.2%) and morulae (1427, 51.9%) obtained by co-culture on caprine cumulus cells (cCC). Some oocytes reached the blastocyst stage (454, 7.4%) following oocyte collection by aspiration and culture on caprine oviduct epithelial cells (cOEC). After 4- and 8-cell stage embryos obtained by aspiration and culture on cCC were transferred pregnancy resulted. Twin male kids (developed from different embryos) were born on August 6, 1993, and have developed into normal bucks. Conditions reported here provided an adequate environment for support of oocyte maturation, fertilization and early embryonic development in vitro (IVMFC) with normal development after embryo transfer." @default.
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• W2149292647 date "1994-01-01" @default.
• W2149292647 modified "2023-09-29" @default.
• W2149292647 title "Term development of caprine embryos derived from immature oocytes in vitro" @default.
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• W2149292647 doi "https://doi.org/10.1016/0093-691x(94)90690-k" @default.
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