FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149297795> ?p ?o ?g. }

• W2149297795 abstract "Virulence of Candida is linked with its ability to form biofilms. Once established, biofilm infections are nearly impossible to eradicate. Biofilm cells live immersed in a self-produced matrix, a blend of extracellular biopolymers, many of which are uncharacterized. In this study, we provide a comprehensive analysis of the matrix manufactured by Candida albicans both in vitro and in a clinical niche animal model. We further explore the function of matrix components, including the impact on drug resistance. We uncovered components from each of the macromolecular classes (55% protein, 25% carbohydrate, 15% lipid, and 5% nucleic acid) in the C. albicans biofilm matrix. Three individual polysaccharides were identified and were suggested to interact physically. Surprisingly, a previously identified polysaccharide of functional importance, β-1,3-glucan, comprised only a small portion of the total matrix carbohydrate. Newly described, more abundant polysaccharides included α-1,2 branched α-1,6-mannans (87%) associated with unbranched β-1,6-glucans (13%) in an apparent mannan-glucan complex (MGCx). Functional matrix proteomic analysis revealed 458 distinct activities. The matrix lipids consisted of neutral glycerolipids (89.1%), polar glycerolipids (10.4%), and sphingolipids (0.5%). Examination of matrix nucleic acid identified DNA, primarily noncoding sequences. Several of the in vitro matrix components, including proteins and each of the polysaccharides, were also present in the matrix of a clinically relevant in vivo biofilm. Nuclear magnetic resonance (NMR) analysis demonstrated interaction of aggregate matrix with the antifungal fluconazole, consistent with a role in drug impedance and contribution of multiple matrix components. Importance: This report is the first to decipher the complex and unique macromolecular composition of the Candida biofilm matrix, demonstrate the clinical relevance of matrix components, and show that multiple matrix components are needed for protection from antifungal drugs. The availability of these biochemical analyses provides a unique resource for further functional investigation of the biofilm matrix, a defining trait of this lifestyle." @default.
• W2149297795 created "2016-06-24" @default.
• W2149297795 creator A5001681130 @default.
• W2149297795 creator A5003491838 @default.
• W2149297795 creator A5008082935 @default.
• W2149297795 creator A5011309930 @default.
• W2149297795 creator A5013506365 @default.
• W2149297795 creator A5019564581 @default.
• W2149297795 creator A5028256879 @default.
• W2149297795 creator A5032606873 @default.
• W2149297795 creator A5042042380 @default.
• W2149297795 creator A5065069084 @default.
• W2149297795 creator A5070122257 @default.
• W2149297795 creator A5070541271 @default.
• W2149297795 creator A5073545620 @default.
• W2149297795 creator A5088276536 @default.
• W2149297795 date "2014-08-29" @default.
• W2149297795 modified "2023-10-05" @default.
• W2149297795 title "Novel Entries in a Fungal Biofilm Matrix Encyclopedia" @default.
• W2149297795 cites W1479770313 @default.
• W2149297795 cites W1901946155 @default.
• W2149297795 cites W1963848474 @default.
• W2149297795 cites W1966552407 @default.
• W2149297795 cites W1967239401 @default.
• W2149297795 cites W1981593008 @default.
• W2149297795 cites W1981609001 @default.
• W2149297795 cites W1982895042 @default.
• W2149297795 cites W1986888461 @default.
• W2149297795 cites W1988402320 @default.
• W2149297795 cites W1991275052 @default.
• W2149297795 cites W1991556610 @default.
• W2149297795 cites W1996937282 @default.
• W2149297795 cites W1998767819 @default.
• W2149297795 cites W1999751604 @default.
• W2149297795 cites W2002221873 @default.
• W2149297795 cites W2003829155 @default.
• W2149297795 cites W2015215410 @default.
• W2149297795 cites W2015593638 @default.
• W2149297795 cites W2020607017 @default.
• W2149297795 cites W2023993099 @default.
• W2149297795 cites W2032059162 @default.
• W2149297795 cites W2036777897 @default.
• W2149297795 cites W2039886457 @default.
• W2149297795 cites W2040004606 @default.
• W2149297795 cites W2040014098 @default.
• W2149297795 cites W2048793078 @default.
• W2149297795 cites W2050694730 @default.
• W2149297795 cites W2055376153 @default.
• W2149297795 cites W2056230398 @default.
• W2149297795 cites W2059111198 @default.
• W2149297795 cites W2059410976 @default.
• W2149297795 cites W2065635384 @default.
• W2149297795 cites W2066976786 @default.
• W2149297795 cites W2070300719 @default.
• W2149297795 cites W2076755375 @default.
• W2149297795 cites W2078144484 @default.
• W2149297795 cites W2079553804 @default.
• W2149297795 cites W2082196711 @default.
• W2149297795 cites W2083250007 @default.
• W2149297795 cites W2085216601 @default.
• W2149297795 cites W2085387269 @default.
• W2149297795 cites W2093430945 @default.
• W2149297795 cites W2098597000 @default.
• W2149297795 cites W2102438460 @default.
• W2149297795 cites W2106769310 @default.
• W2149297795 cites W2107531307 @default.
• W2149297795 cites W2109434556 @default.
• W2149297795 cites W2112078820 @default.
• W2149297795 cites W2113391818 @default.
• W2149297795 cites W2115933619 @default.
• W2149297795 cites W2116394016 @default.
• W2149297795 cites W2120978086 @default.
• W2149297795 cites W2122089681 @default.
• W2149297795 cites W2122115838 @default.
• W2149297795 cites W2124008198 @default.
• W2149297795 cites W2124380402 @default.
• W2149297795 cites W2124954220 @default.
• W2149297795 cites W2125375689 @default.
• W2149297795 cites W2126398585 @default.
• W2149297795 cites W2127725984 @default.
• W2149297795 cites W2129629034 @default.
• W2149297795 cites W2130763979 @default.
• W2149297795 cites W2131727615 @default.
• W2149297795 cites W2134023938 @default.
• W2149297795 cites W2136195640 @default.
• W2149297795 cites W2137940909 @default.
• W2149297795 cites W2138134673 @default.
• W2149297795 cites W2141563676 @default.
• W2149297795 cites W2144440398 @default.
• W2149297795 cites W2146787746 @default.
• W2149297795 cites W2149218811 @default.
• W2149297795 cites W2149606831 @default.
• W2149297795 cites W2150953954 @default.
• W2149297795 cites W2152042119 @default.
• W2149297795 cites W2154143400 @default.
• W2149297795 cites W2155894000 @default.
• W2149297795 cites W2157946772 @default.
• W2149297795 cites W2159297496 @default.
• W2149297795 cites W2163811015 @default.
• W2149297795 cites W2165925548 @default.

• next