Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149325862> ?p ?o ?g. }
- W2149325862 abstract "It is widely believed that integral outer membrane (OM) proteins in bacteria are able to diffuse laterally in the OM. However, stable, immobile proteins have been identified in the OM of Escherichia coli. In explaining the observations, a hypothesized interaction of the immobilized OM proteins with the underlying peptidoglycan (PG) cell wall played a prominent role.OmpA is an abundant outer membrane protein in E. coli containing a PG-binding domain. We use FRAP to investigate whether OmpA is able to diffuse laterally over long-range (> ~100 nm) distances in the OM. First, we show that OmpA, containing a PG binding domain, does not exhibit long-range lateral diffusion in the OM. Then, to test whether PG interaction was required for this immobilization, we genetically removed the PG binding domain and repeated the FRAP experiment. To our surprise, this did not increase the mobility of the protein in the OM.OmpA exhibits an absence of long-range (> ~100 nm) diffusion in the OM that is not caused by its PG binding domain. Therefore, other mechanisms are needed to explain this observation, such as the presence of physical barriers in the OM, or strong interactions with other elements in the cell envelope." @default.
- W2149325862 created "2016-06-24" @default.
- W2149325862 creator A5011932505 @default.
- W2149325862 creator A5079496608 @default.
- W2149325862 creator A5084091962 @default.
- W2149325862 date "2013-03-23" @default.
- W2149325862 modified "2023-10-17" @default.
- W2149325862 title "Absence of long-range diffusion of OmpA in E. coliis not caused by its peptidoglycan binding domain" @default.
- W2149325862 cites W1490701934 @default.
- W2149325862 cites W1531386379 @default.
- W2149325862 cites W1532818455 @default.
- W2149325862 cites W1560081729 @default.
- W2149325862 cites W1671789228 @default.
- W2149325862 cites W1781531090 @default.
- W2149325862 cites W1865108114 @default.
- W2149325862 cites W1981483220 @default.
- W2149325862 cites W1992003720 @default.
- W2149325862 cites W2009918551 @default.
- W2149325862 cites W2024526152 @default.
- W2149325862 cites W2025431751 @default.
- W2149325862 cites W2029158435 @default.
- W2149325862 cites W2040549857 @default.
- W2149325862 cites W2041654055 @default.
- W2149325862 cites W2051416943 @default.
- W2149325862 cites W2059784037 @default.
- W2149325862 cites W2061332018 @default.
- W2149325862 cites W2061628951 @default.
- W2149325862 cites W2096650678 @default.
- W2149325862 cites W2107217578 @default.
- W2149325862 cites W2111375449 @default.
- W2149325862 cites W2113098502 @default.
- W2149325862 cites W2115513456 @default.
- W2149325862 cites W2115734610 @default.
- W2149325862 cites W2118546914 @default.
- W2149325862 cites W2120061656 @default.
- W2149325862 cites W2121839643 @default.
- W2149325862 cites W2132929413 @default.
- W2149325862 cites W2148641266 @default.
- W2149325862 cites W2150010294 @default.
- W2149325862 cites W2150712367 @default.
- W2149325862 cites W2158550334 @default.
- W2149325862 cites W2160398772 @default.
- W2149325862 cites W2169242133 @default.
- W2149325862 cites W2171539493 @default.
- W2149325862 cites W41179907 @default.
- W2149325862 doi "https://doi.org/10.1186/1471-2180-13-66" @default.
- W2149325862 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3637615" @default.
- W2149325862 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23522061" @default.
- W2149325862 hasPublicationYear "2013" @default.
- W2149325862 type Work @default.
- W2149325862 sameAs 2149325862 @default.
- W2149325862 citedByCount "19" @default.
- W2149325862 countsByYear W21493258622014 @default.
- W2149325862 countsByYear W21493258622015 @default.
- W2149325862 countsByYear W21493258622016 @default.
- W2149325862 countsByYear W21493258622017 @default.
- W2149325862 countsByYear W21493258622019 @default.
- W2149325862 countsByYear W21493258622020 @default.
- W2149325862 countsByYear W21493258622021 @default.
- W2149325862 countsByYear W21493258622022 @default.
- W2149325862 countsByYear W21493258622023 @default.
- W2149325862 crossrefType "journal-article" @default.
- W2149325862 hasAuthorship W2149325862A5011932505 @default.
- W2149325862 hasAuthorship W2149325862A5079496608 @default.
- W2149325862 hasAuthorship W2149325862A5084091962 @default.
- W2149325862 hasBestOaLocation W21493258621 @default.
- W2149325862 hasConcept C104317684 @default.
- W2149325862 hasConcept C107824862 @default.
- W2149325862 hasConcept C125235067 @default.
- W2149325862 hasConcept C12554922 @default.
- W2149325862 hasConcept C146587185 @default.
- W2149325862 hasConcept C25166345 @default.
- W2149325862 hasConcept C2776634448 @default.
- W2149325862 hasConcept C2778106830 @default.
- W2149325862 hasConcept C41625074 @default.
- W2149325862 hasConcept C46371251 @default.
- W2149325862 hasConcept C51639874 @default.
- W2149325862 hasConcept C547475151 @default.
- W2149325862 hasConcept C553089730 @default.
- W2149325862 hasConcept C55493867 @default.
- W2149325862 hasConcept C86803240 @default.
- W2149325862 hasConceptScore W2149325862C104317684 @default.
- W2149325862 hasConceptScore W2149325862C107824862 @default.
- W2149325862 hasConceptScore W2149325862C125235067 @default.
- W2149325862 hasConceptScore W2149325862C12554922 @default.
- W2149325862 hasConceptScore W2149325862C146587185 @default.
- W2149325862 hasConceptScore W2149325862C25166345 @default.
- W2149325862 hasConceptScore W2149325862C2776634448 @default.
- W2149325862 hasConceptScore W2149325862C2778106830 @default.
- W2149325862 hasConceptScore W2149325862C41625074 @default.
- W2149325862 hasConceptScore W2149325862C46371251 @default.
- W2149325862 hasConceptScore W2149325862C51639874 @default.
- W2149325862 hasConceptScore W2149325862C547475151 @default.
- W2149325862 hasConceptScore W2149325862C553089730 @default.
- W2149325862 hasConceptScore W2149325862C55493867 @default.
- W2149325862 hasConceptScore W2149325862C86803240 @default.
- W2149325862 hasIssue "1" @default.
- W2149325862 hasLocation W21493258621 @default.
- W2149325862 hasLocation W21493258622 @default.
- W2149325862 hasLocation W21493258623 @default.