FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149341739> ?p ?o ?g. }

• W2149341739 endingPage "R133" @default.
• W2149341739 startingPage "R133" @default.
• W2149341739 abstract "Autologous chondrocyte transplantation (ACT) is a routine technique to regenerate focal cartilage lesions. However, patients with osteoarthritis (OA) are lacking an appropriate long-lasting treatment alternative, partly since it is not known if chondrocytes from OA patients have the same chondrogenic differentiation potential as chondrocytes from donors not affected by OA. Articular chondrocytes from patients with OA undergoing total knee replacement (Mankin Score > 3, Ahlbäck Score > 2) and from patients undergoing ACT, here referred to as normal donors (ND), were isolated applying protocols used for ACT. Their chondrogenic differentiation potential was evaluated both in high-density pellet and scaffold (Hyaff-11) cultures by histological proteoglycan assessment (Bern Score) and immunohistochemistry for collagen types I and II. Chondrocytes cultured in monolayer and scaffolds were subjected to gene expression profiling using genome-wide oligonucleotide microarrays. Expression data were verified by using real-time PCR. Chondrocytes from ND and OA donors demonstrated accumulation of comparable amounts of cartilage matrix components, including sulphated proteoglycans and collagen types I and II. The mRNA expression of cartilage markers (ACAN, COL2A1, COMP, CRTL1, SOX9) and genes involved in matrix synthesis (BGN, CILP2, COL9A2, COL11A1, TIMP4) was highly induced in 3D cultures of chondrocytes from both donor groups. Genes associated with hypertrophic or OA cartilage (ALPL, COL1A1, COL3A1, COL10A1, MMP13, POSTN, PTH1R, RUNX2) were not significantly regulated between the two groups of donors. The expression of 661 genes, including COMP, FN1, and SOX9, was differentially regulated between OA and ND chondrocytes cultured in monolayer. During scaffold culture, the differences diminished between the OA and ND chondrocytes, and only 184 genes were differentially regulated. Only few genes were differentially expressed between OA and ND chondrocytes in Hyaff-11 culture. The risk of differentiation into hypertrophic cartilage does not seem to be increased for OA chondrocytes. Our findings suggest that the chondrogenic capacity is not significantly affected by OA, and OA chondrocytes fulfill the requirements for matrix-associated ACT." @default.
• W2149341739 created "2016-06-24" @default.
• W2149341739 creator A5007869667 @default.
• W2149341739 creator A5028238778 @default.
• W2149341739 creator A5038061458 @default.
• W2149341739 creator A5038842105 @default.
• W2149341739 creator A5041215843 @default.
• W2149341739 date "2009-01-01" @default.
• W2149341739 modified "2023-10-18" @default.
• W2149341739 title "Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation" @default.
• W2149341739 cites W1554947160 @default.
• W2149341739 cites W1561858686 @default.
• W2149341739 cites W1827975139 @default.
• W2149341739 cites W192836234 @default.
• W2149341739 cites W1965172266 @default.
• W2149341739 cites W1965970260 @default.
• W2149341739 cites W1966356232 @default.
• W2149341739 cites W1970839075 @default.
• W2149341739 cites W1981935743 @default.
• W2149341739 cites W1985276061 @default.
• W2149341739 cites W1986409534 @default.
• W2149341739 cites W1988592246 @default.
• W2149341739 cites W2000571117 @default.
• W2149341739 cites W2002963723 @default.
• W2149341739 cites W2007227967 @default.
• W2149341739 cites W2009264513 @default.
• W2149341739 cites W2011693143 @default.
• W2149341739 cites W2015354603 @default.
• W2149341739 cites W2022130870 @default.
• W2149341739 cites W2023156173 @default.
• W2149341739 cites W2027580325 @default.
• W2149341739 cites W2027600661 @default.
• W2149341739 cites W2029721393 @default.
• W2149341739 cites W2030568617 @default.
• W2149341739 cites W2037755817 @default.
• W2149341739 cites W2038243239 @default.
• W2149341739 cites W2038866653 @default.
• W2149341739 cites W2040325888 @default.
• W2149341739 cites W2050911192 @default.
• W2149341739 cites W2060838279 @default.
• W2149341739 cites W2066687881 @default.
• W2149341739 cites W2067131758 @default.
• W2149341739 cites W2078744277 @default.
• W2149341739 cites W2079102658 @default.
• W2149341739 cites W2081418368 @default.
• W2149341739 cites W2093369644 @default.
• W2149341739 cites W2097399478 @default.
• W2149341739 cites W2098810887 @default.
• W2149341739 cites W2099669252 @default.
• W2149341739 cites W2108244474 @default.
• W2149341739 cites W2108491719 @default.
• W2149341739 cites W2113457553 @default.
• W2149341739 cites W2125361012 @default.
• W2149341739 cites W2127324240 @default.
• W2149341739 cites W2141591906 @default.
• W2149341739 cites W2146992068 @default.
• W2149341739 cites W2152217106 @default.
• W2149341739 cites W2159369930 @default.
• W2149341739 cites W2168890961 @default.
• W2149341739 cites W2170984819 @default.
• W2149341739 cites W2401104106 @default.
• W2149341739 cites W2405122236 @default.
• W2149341739 cites W3022355476 @default.
• W2149341739 doi "https://doi.org/10.1186/ar2800" @default.
• W2149341739 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2787268" @default.
• W2149341739 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19723327" @default.
• W2149341739 hasPublicationYear "2009" @default.
• W2149341739 type Work @default.
• W2149341739 sameAs 2149341739 @default.
• W2149341739 citedByCount "119" @default.
• W2149341739 countsByYear W21493417392012 @default.
• W2149341739 countsByYear W21493417392013 @default.
• W2149341739 countsByYear W21493417392014 @default.
• W2149341739 countsByYear W21493417392015 @default.
• W2149341739 countsByYear W21493417392016 @default.
• W2149341739 countsByYear W21493417392017 @default.
• W2149341739 countsByYear W21493417392018 @default.
• W2149341739 countsByYear W21493417392019 @default.
• W2149341739 countsByYear W21493417392020 @default.
• W2149341739 countsByYear W21493417392021 @default.
• W2149341739 countsByYear W21493417392022 @default.
• W2149341739 countsByYear W21493417392023 @default.
• W2149341739 crossrefType "journal-article" @default.
• W2149341739 hasAuthorship W2149341739A5007869667 @default.
• W2149341739 hasAuthorship W2149341739A5028238778 @default.
• W2149341739 hasAuthorship W2149341739A5038061458 @default.
• W2149341739 hasAuthorship W2149341739A5038842105 @default.
• W2149341739 hasAuthorship W2149341739A5041215843 @default.
• W2149341739 hasBestOaLocation W21493417391 @default.
• W2149341739 hasConcept C104317684 @default.
• W2149341739 hasConcept C105702510 @default.
• W2149341739 hasConcept C126322002 @default.
• W2149341739 hasConcept C142724271 @default.
• W2149341739 hasConcept C150194340 @default.
• W2149341739 hasConcept C185592680 @default.
• W2149341739 hasConcept C197534660 @default.
• W2149341739 hasConcept C204787440 @default.
• W2149341739 hasConcept C2776164576 @default.

• next