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• W2149354404 abstract "A simple PCR method was developed for the detection of Marek's disease (MD) and reticuloendotheliosis (RE) in formalin-fixed paraffin-embedded (FFPE) tissues, and for the detection of MD in tissues only preserved in 10% neutral buffered formalin. MD virus (MDV) and RE virus proviral DNA were detected in FFPE tissues stored for over 20 yr. MDV was also detected in tissues only preserved in formalin for up to 6 mo. The data indicate that PCR of formalin-fixed and FFPE tissues is a simple and valuable tool that can be used to identify MD and RE infection. The method described in this paper is a good alternative to any biologic or immunohistochemical assay to confirm the detection of MD and RE, as it does not require shipping frozen tissues to the diagnostic laboratory.Nota de Investigación—Aplicación de la reacción en cadena de la polimerasa para la detección de los virus de la enfermedad de Marek y de la reticuloendoteliosis en tejidos tumorales fijados en formalina e incluidos en parafina.Se desarrolló un método simple de PCR para la detección de la enfermedad de Marek (MD) y de la reticuloendoteliosis (ER) en tejidos fijados con formalina e incluidos en parafina (FFPE) y para la detección del virus de Marek únicamente en los tejidos conservados en formalina al 10% neutra y amortiguada. Se detectaron los virus de Marek y el ADN proviral del virus de la reticuloendoteliosis en tejidos fijados e incluidos en parafina que estaban almacenados durante más de 20 años. El virus de Marek también se detectó en los tejidos que estaban únicamente conservados en formol por hasta seis meses. Los datos indican que el método de PCR utilizando tejidos fijados con formalina e incluidos en parafina es una herramienta simple y valiosa que se puede utilizar para identificar al virus de la enfermedad de Marek y de la reticuloendoteliosis. El método descrito en este documento es una buena alternativa para cualquier ensayo biológico o inmunohistoquímico para confirmar la detección de estos dos virus, ya que no requiere del envío de tejidos congelados al laboratorio de diagnóstico." @default.
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• W2149354404 date "2013-12-01" @default.
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• W2149354404 title "Use of Polymerase Chain Reaction in Detection of Marek's Disease and Reticuloendotheliosis Viruses in Formalin-Fixed, Paraffin-Embedded Tumorous Tissues" @default.
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• W2149354404 doi "https://doi.org/10.1637/10542-032713-resnote.1" @default.
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