Matches in SemOpenAlex for { <https://semopenalex.org/work/W214936872> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W214936872 abstract "cGMP binding proteins in toad ROS were identified by direct photoaffinity labeling (PAL) with /sup 32/P-cGMP and quantified by retention of complexes on nitrocellulose filters. By PAL, high affinity sites were present on the ..cap alpha.. and ..beta.. subunits of the cGMP-specific phosphodiesterase (PDE) which have MW/sub app/ of 94 and 90 kDa. A doublet was deduced from its photolabeling properties to represent PDE/sub ..gamma../ photocrosslinked with PDE/sub ..cap alpha../ or PDE/sub ..beta../, respectively. cGMP prebound to these high affinity sites was released by light-activated G-protein or its ..cap alpha.. subunit complexed with GTP..gamma..S; this inhibition of cGMP binding to PDE did not result from decreased cGMP availability due to enhanced hydrolysis. A low affinity cGMP binding component identified by PAL is tightly associated with ROS membranes. Apparent ATP/light-dependent stimulation of cGMP binding was shown to result from light activated cGMP hydrolysis in conjunction with ATP-promoted conversion of GMP to GDP/GTP and increased GDP/GTP binding. These findings coincide with a model for light-related regulation of cGMP binding and metabolism predicted from intact and cellfree kinetic measurements: in the dark state the cGMP hydrolic rate is constrained by the availability of cGMP because of its binding to high affinity sites onmore » PDE. Light activated G-protein releases cGMP from these sites and allows for its redistribution to lower affinity sites represented by PDE catalytic site(s) and possible cGMP-dependent membrane cation channels.« less" @default.
- W214936872 created "2016-06-24" @default.
- W214936872 creator A5003661152 @default.
- W214936872 creator A5017566641 @default.
- W214936872 creator A5036817167 @default.
- W214936872 creator A5058259419 @default.
- W214936872 creator A5058684573 @default.
- W214936872 creator A5059131762 @default.
- W214936872 date "1987-05-01" @default.
- W214936872 modified "2023-09-26" @default.
- W214936872 title "Activated G-protein releases cGMP from high affinity binding sites on PDE from toad rod outer segments (ROS)" @default.
- W214936872 hasPublicationYear "1987" @default.
- W214936872 type Work @default.
- W214936872 sameAs 214936872 @default.
- W214936872 citedByCount "0" @default.
- W214936872 crossrefType "journal-article" @default.
- W214936872 hasAuthorship W214936872A5003661152 @default.
- W214936872 hasAuthorship W214936872A5017566641 @default.
- W214936872 hasAuthorship W214936872A5036817167 @default.
- W214936872 hasAuthorship W214936872A5058259419 @default.
- W214936872 hasAuthorship W214936872A5058684573 @default.
- W214936872 hasAuthorship W214936872A5059131762 @default.
- W214936872 hasConcept C107824862 @default.
- W214936872 hasConcept C118716 @default.
- W214936872 hasConcept C12554922 @default.
- W214936872 hasConcept C134018914 @default.
- W214936872 hasConcept C181199279 @default.
- W214936872 hasConcept C185592680 @default.
- W214936872 hasConcept C2778166480 @default.
- W214936872 hasConcept C55493867 @default.
- W214936872 hasConcept C58141971 @default.
- W214936872 hasConcept C62826618 @default.
- W214936872 hasConcept C79418042 @default.
- W214936872 hasConcept C86803240 @default.
- W214936872 hasConceptScore W214936872C107824862 @default.
- W214936872 hasConceptScore W214936872C118716 @default.
- W214936872 hasConceptScore W214936872C12554922 @default.
- W214936872 hasConceptScore W214936872C134018914 @default.
- W214936872 hasConceptScore W214936872C181199279 @default.
- W214936872 hasConceptScore W214936872C185592680 @default.
- W214936872 hasConceptScore W214936872C2778166480 @default.
- W214936872 hasConceptScore W214936872C55493867 @default.
- W214936872 hasConceptScore W214936872C58141971 @default.
- W214936872 hasConceptScore W214936872C62826618 @default.
- W214936872 hasConceptScore W214936872C79418042 @default.
- W214936872 hasConceptScore W214936872C86803240 @default.
- W214936872 hasLocation W2149368721 @default.
- W214936872 hasOpenAccess W214936872 @default.
- W214936872 hasPrimaryLocation W2149368721 @default.
- W214936872 hasRelatedWork W1485225530 @default.
- W214936872 hasRelatedWork W1528621273 @default.
- W214936872 hasRelatedWork W1549375032 @default.
- W214936872 hasRelatedWork W1570624812 @default.
- W214936872 hasRelatedWork W1571104445 @default.
- W214936872 hasRelatedWork W1639941291 @default.
- W214936872 hasRelatedWork W1926371675 @default.
- W214936872 hasRelatedWork W1965205594 @default.
- W214936872 hasRelatedWork W1967443949 @default.
- W214936872 hasRelatedWork W1988565280 @default.
- W214936872 hasRelatedWork W2000753313 @default.
- W214936872 hasRelatedWork W2005320838 @default.
- W214936872 hasRelatedWork W2006640518 @default.
- W214936872 hasRelatedWork W2019107900 @default.
- W214936872 hasRelatedWork W2058848181 @default.
- W214936872 hasRelatedWork W2069017869 @default.
- W214936872 hasRelatedWork W2395172023 @default.
- W214936872 hasRelatedWork W2413718792 @default.
- W214936872 hasRelatedWork W2436283272 @default.
- W214936872 hasRelatedWork W3133597113 @default.
- W214936872 isParatext "false" @default.
- W214936872 isRetracted "false" @default.
- W214936872 magId "214936872" @default.
- W214936872 workType "article" @default.